Microbacterium ginsengiterrae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A419XZV6|A0A419XZV6_9MICO Putative repeat protein (TIGR03943 family) OS=Microbacterium ginsengiterrae OX=546115 GN=C7323_0272 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84LPALRR7 pKa = 11.84LTGAAAVVLTTAAALVGCAPAPTAPEE33 pKa = 3.78LSGDD37 pKa = 4.09VFVHH41 pKa = 6.57DD42 pKa = 3.89PAYY45 pKa = 10.54AVTDD49 pKa = 4.04DD50 pKa = 3.77GSFVYY55 pKa = 9.45STGNGQIADD64 pKa = 3.73GNIQIRR70 pKa = 11.84RR71 pKa = 11.84SDD73 pKa = 4.59DD74 pKa = 3.58GADD77 pKa = 2.9WEE79 pKa = 4.72YY80 pKa = 11.48AGEE83 pKa = 4.23VWDD86 pKa = 5.45TKK88 pKa = 10.28PAWLGEE94 pKa = 4.06AVPGVDD100 pKa = 4.29NLWAPEE106 pKa = 4.15LYY108 pKa = 10.37EE109 pKa = 6.22HH110 pKa = 7.81DD111 pKa = 4.15GTWYY115 pKa = 10.61LYY117 pKa = 10.83YY118 pKa = 10.42SASTFGSNVSVIALATNEE136 pKa = 4.4TLDD139 pKa = 3.94PADD142 pKa = 4.59PAFEE146 pKa = 4.28WVDD149 pKa = 3.51QGPVIASEE157 pKa = 4.22STDD160 pKa = 3.81FNAIDD165 pKa = 3.72PGIVEE170 pKa = 4.89DD171 pKa = 5.29ADD173 pKa = 3.85GEE175 pKa = 4.12PWMAFGSFSSGLQIVPLQWPSGLRR199 pKa = 11.84ADD201 pKa = 3.85EE202 pKa = 4.69SEE204 pKa = 5.15PITIADD210 pKa = 3.53RR211 pKa = 11.84GLRR214 pKa = 11.84EE215 pKa = 3.99NAIEE219 pKa = 4.03APYY222 pKa = 10.12IVPHH226 pKa = 7.13DD227 pKa = 3.5GAFYY231 pKa = 10.88LFASRR236 pKa = 11.84GFCCRR241 pKa = 11.84GVDD244 pKa = 3.46STYY247 pKa = 10.7EE248 pKa = 4.12IIVGRR253 pKa = 11.84ADD255 pKa = 3.72SVTGPYY261 pKa = 10.4LDD263 pKa = 5.24ADD265 pKa = 4.27DD266 pKa = 5.22GALLEE271 pKa = 5.19DD272 pKa = 4.42GGTVVLTSEE281 pKa = 5.0GSRR284 pKa = 11.84VGPGGQSVSGDD295 pKa = 3.05VLAFHH300 pKa = 7.5FYY302 pKa = 10.89DD303 pKa = 4.61ADD305 pKa = 3.95ADD307 pKa = 4.62GIPTLGLQPIVWRR320 pKa = 11.84DD321 pKa = 3.14GWPTVVEE328 pKa = 4.27

Molecular weight: 34.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A419Y2I3|A0A419Y2I3_9MICO DNA-binding transcriptional MerR regulator OS=Microbacterium ginsengiterrae OX=546115 GN=C7323_1226 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3754

0

3754

1274925

30

2824

339.6

36.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.649 ± 0.057

0.467 ± 0.009

6.435 ± 0.037

5.755 ± 0.033

3.207 ± 0.024

8.834 ± 0.034

1.929 ± 0.017

4.933 ± 0.029

1.764 ± 0.028

10.074 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.795 ± 0.016

1.958 ± 0.021

5.262 ± 0.029

2.803 ± 0.02

7.153 ± 0.046

5.612 ± 0.028

6.038 ± 0.041

8.725 ± 0.035

1.591 ± 0.017

2.014 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski