Babesia bigemina

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Piroplasmida; Babesiidae; Babesia

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4432 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A061D4N0|A0A061D4N0_BABBI RAP domain-containing protein OS=Babesia bigemina OX=5866 GN=BBBOND_0208250 PE=4 SV=1
MM1 pKa = 7.7HH2 pKa = 7.56KK3 pKa = 9.95PQAVNQYY10 pKa = 9.91EE11 pKa = 3.98NDD13 pKa = 3.73DD14 pKa = 4.56GYY16 pKa = 11.52DD17 pKa = 3.43EE18 pKa = 6.27GDD20 pKa = 5.23DD21 pKa = 5.41GDD23 pKa = 6.23DD24 pKa = 5.43DD25 pKa = 6.85DD26 pKa = 7.66DD27 pKa = 6.95DD28 pKa = 6.68DD29 pKa = 7.13GEE31 pKa = 5.11DD32 pKa = 3.47GHH34 pKa = 6.78LTAHH38 pKa = 6.96IPHH41 pKa = 6.84PVEE44 pKa = 4.89RR45 pKa = 11.84PDD47 pKa = 4.89AEE49 pKa = 4.22VDD51 pKa = 3.22HH52 pKa = 7.35DD53 pKa = 5.75ADD55 pKa = 5.14DD56 pKa = 6.17DD57 pKa = 4.64DD58 pKa = 6.73DD59 pKa = 4.77EE60 pKa = 7.32GYY62 pKa = 11.47GDD64 pKa = 4.34DD65 pKa = 5.1DD66 pKa = 4.27VRR68 pKa = 11.84GGNYY72 pKa = 9.75GHH74 pKa = 7.25LTAHH78 pKa = 6.68VICHH82 pKa = 5.12QRR84 pKa = 11.84NHH86 pKa = 5.95YY87 pKa = 10.31SVV89 pKa = 3.37

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A061DDH9|A0A061DDH9_BABBI Uncharacterized protein OS=Babesia bigemina OX=5866 GN=BBBOND_0301840 PE=4 SV=1
MM1 pKa = 7.52VYY3 pKa = 8.48THH5 pKa = 7.28FGRR8 pKa = 11.84ITKK11 pKa = 7.9QWCGSTHH18 pKa = 6.12LRR20 pKa = 11.84IGSRR24 pKa = 11.84GQPRR28 pKa = 11.84PNKK31 pKa = 9.5GFSVKK36 pKa = 10.46HH37 pKa = 5.79FDD39 pKa = 3.75TVGSALEE46 pKa = 4.05CCRR49 pKa = 11.84WWKK52 pKa = 9.63FLRR55 pKa = 11.84RR56 pKa = 11.84GTLARR61 pKa = 11.84ILNLQSSSAEE71 pKa = 3.56ASVPGSCQCALLMQLVVTSTHH92 pKa = 4.34IHH94 pKa = 5.0NVEE97 pKa = 3.99NGSAKK102 pKa = 10.81LNDD105 pKa = 3.55QQLSVLSSYY114 pKa = 10.95LGVSFQPRR122 pKa = 11.84KK123 pKa = 8.54APRR126 pKa = 11.84YY127 pKa = 9.26RR128 pKa = 11.84RR129 pKa = 11.84II130 pKa = 3.35

Molecular weight: 14.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4432

0

4432

2259908

51

4986

509.9

56.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.874 ± 0.033

2.182 ± 0.02

6.019 ± 0.03

6.262 ± 0.04

3.681 ± 0.021

6.038 ± 0.027

2.628 ± 0.016

4.998 ± 0.028

5.691 ± 0.049

9.269 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.019

4.227 ± 0.023

4.624 ± 0.034

3.666 ± 0.024

5.958 ± 0.033

7.906 ± 0.034

5.484 ± 0.023

6.847 ± 0.028

0.978 ± 0.01

3.218 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski