Winogradskyella sp. KYW1333

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella; unclassified Winogradskyella

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2480 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A368MDQ9|A0A368MDQ9_9FLAO Low molecular weight phosphotyrosine protein phosphatase OS=Winogradskyella sp. KYW1333 OX=2282123 GN=DUZ96_09670 PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.16FYY4 pKa = 10.97QLTLYY9 pKa = 10.44LLSINLSVAQIAFDD23 pKa = 4.19NEE25 pKa = 4.09AEE27 pKa = 4.28TLGVEE32 pKa = 4.66IICGNTLFGNGVSFFDD48 pKa = 4.22YY49 pKa = 11.27DD50 pKa = 3.47NDD52 pKa = 3.52GWDD55 pKa = 4.19DD56 pKa = 3.95LTVATADD63 pKa = 3.79DD64 pKa = 4.36DD65 pKa = 4.01PVRR68 pKa = 11.84FYY70 pKa = 11.87KK71 pKa = 10.66NINGNFVEE79 pKa = 4.33QTLNIANNNWRR90 pKa = 11.84NKK92 pKa = 7.23QVNWVDD98 pKa = 3.06IDD100 pKa = 3.84NDD102 pKa = 3.57GDD104 pKa = 3.59NDD106 pKa = 4.35LFVTSDD112 pKa = 3.06TSINRR117 pKa = 11.84LYY119 pKa = 11.02EE120 pKa = 3.84NLGNMILQDD129 pKa = 3.35ITSTSGLLNEE139 pKa = 4.3VLVTYY144 pKa = 9.26GASWGDD150 pKa = 3.61YY151 pKa = 10.85NNDD154 pKa = 2.74GFLDD158 pKa = 3.77VFLSNRR164 pKa = 11.84DD165 pKa = 3.4TTIPNILYY173 pKa = 10.41KK174 pKa = 11.14NNGNNTFSLVNSEE187 pKa = 4.61SGLITTGTLSFCSAFIDD204 pKa = 3.95YY205 pKa = 10.98NKK207 pKa = 10.95DD208 pKa = 3.05GFQDD212 pKa = 3.3IYY214 pKa = 10.95VANDD218 pKa = 2.87KK219 pKa = 11.1VEE221 pKa = 4.22YY222 pKa = 9.54PNIMYY227 pKa = 10.48KK228 pKa = 10.85NNGDD232 pKa = 3.65GTFTEE237 pKa = 4.82VGFEE241 pKa = 4.03TGTDD245 pKa = 3.13ITIDD249 pKa = 3.47AMSVTIGDD257 pKa = 4.05YY258 pKa = 11.34NSDD261 pKa = 3.52SWLDD265 pKa = 3.28IYY267 pKa = 9.62VTNDD271 pKa = 2.85VVGNVLLKK279 pKa = 11.16NNGNGTFSDD288 pKa = 3.8EE289 pKa = 4.35TLSSNTAMNSVAWGSVFLDD308 pKa = 3.71AEE310 pKa = 4.23NDD312 pKa = 3.57GDD314 pKa = 4.22LDD316 pKa = 3.79IYY318 pKa = 11.37VSAEE322 pKa = 3.55HH323 pKa = 7.24DD324 pKa = 3.94GTVSGYY330 pKa = 10.69LSSALFTNNNDD341 pKa = 3.09EE342 pKa = 4.65YY343 pKa = 10.71ILEE346 pKa = 4.39NEE348 pKa = 5.39AIPDD352 pKa = 4.08DD353 pKa = 4.1YY354 pKa = 11.55AISYY358 pKa = 10.51SNAIGDD364 pKa = 4.2IDD366 pKa = 4.12NDD368 pKa = 4.46GYY370 pKa = 10.44PEE372 pKa = 3.9ILVNNINHH380 pKa = 6.97DD381 pKa = 5.11NIFLWKK387 pKa = 10.51NNSNNTNNWLKK398 pKa = 10.99VKK400 pKa = 11.03LEE402 pKa = 3.99GTISNRR408 pKa = 11.84NGVGSMIEE416 pKa = 3.55ISVNGEE422 pKa = 3.36KK423 pKa = 10.22QYY425 pKa = 11.04RR426 pKa = 11.84YY427 pKa = 7.6TLIGEE432 pKa = 5.23GYY434 pKa = 10.25LSQNSASEE442 pKa = 3.84IFGIGDD448 pKa = 3.53ATVIDD453 pKa = 4.18YY454 pKa = 11.05VKK456 pKa = 10.94VNWLSGTEE464 pKa = 4.0DD465 pKa = 2.44IFYY468 pKa = 10.87NVSPNQNLKK477 pKa = 9.89IVEE480 pKa = 4.24GSSLSIEE487 pKa = 4.2EE488 pKa = 4.76SNTSEE493 pKa = 3.95IKK495 pKa = 10.65LFPIPTTEE503 pKa = 3.55ILNIEE508 pKa = 4.01SSLPIEE514 pKa = 4.06EE515 pKa = 4.07VMVYY519 pKa = 10.93NLLGEE524 pKa = 4.44VNISANMNKK533 pKa = 9.71NEE535 pKa = 3.85ISLDD539 pKa = 3.74VTSLEE544 pKa = 3.78PGIYY548 pKa = 10.11FLRR551 pKa = 11.84LSLNSSQEE559 pKa = 3.56IIKK562 pKa = 9.88FIKK565 pKa = 9.96KK566 pKa = 9.87

Molecular weight: 62.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A368MIZ7|A0A368MIZ7_9FLAO Glycoside hydrolase family 16 protein OS=Winogradskyella sp. KYW1333 OX=2282123 GN=DUZ96_01740 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.18RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.6LSVSSEE48 pKa = 4.04SRR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.69KK53 pKa = 9.55

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2480

0

2480

859683

23

2737

346.6

39.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.99 ± 0.051

0.755 ± 0.014

5.989 ± 0.04

6.54 ± 0.044

5.277 ± 0.044

6.456 ± 0.046

1.712 ± 0.023

8.244 ± 0.039

7.479 ± 0.066

9.159 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.142 ± 0.025

6.552 ± 0.052

3.318 ± 0.031

3.185 ± 0.026

3.494 ± 0.033

6.663 ± 0.041

5.677 ± 0.047

6.203 ± 0.036

1.045 ± 0.019

4.119 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski