Microbacterium phage Cressida

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mementomorivirus; unclassified Mementomorivirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514DI82|A0A514DI82_9CAUD HNH endonuclease OS=Microbacterium phage Cressida OX=2591216 GN=55 PE=4 SV=1
MM1 pKa = 7.58TDD3 pKa = 3.86YY4 pKa = 10.89IRR6 pKa = 11.84LAEE9 pKa = 4.29APQWSYY15 pKa = 12.04SSPTCTACTVDD26 pKa = 3.76LTHH29 pKa = 7.79DD30 pKa = 3.98GDD32 pKa = 3.52EE33 pKa = 4.42WMCPVCGTTWPSDD46 pKa = 3.37ATDD49 pKa = 4.18GDD51 pKa = 4.53DD52 pKa = 3.48GTLYY56 pKa = 10.65EE57 pKa = 4.3EE58 pKa = 4.64WSGEE62 pKa = 4.26SLGDD66 pKa = 3.94APTLDD71 pKa = 3.75EE72 pKa = 4.89SAASDD77 pKa = 2.9AGYY80 pKa = 10.72AFEE83 pKa = 3.94RR84 pKa = 11.84AEE86 pKa = 3.91RR87 pKa = 11.84KK88 pKa = 9.82RR89 pKa = 11.84FFLSIGWCEE98 pKa = 3.59HH99 pKa = 5.27GMPGEE104 pKa = 4.45CARR107 pKa = 11.84RR108 pKa = 11.84DD109 pKa = 3.85CPGGTKK115 pKa = 10.41GDD117 pKa = 4.05TPNPDD122 pKa = 2.8

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514DI49|A0A514DI49_9CAUD Uncharacterized protein OS=Microbacterium phage Cressida OX=2591216 GN=31 PE=4 SV=1
MM1 pKa = 7.61SSQFRR6 pKa = 11.84DD7 pKa = 3.35PAAAARR13 pKa = 11.84WVKK16 pKa = 10.09RR17 pKa = 11.84RR18 pKa = 11.84QEE20 pKa = 4.1TAPGTGKK27 pKa = 10.51SIRR30 pKa = 11.84RR31 pKa = 11.84GHH33 pKa = 6.08DD34 pKa = 2.75TWPPPIRR41 pKa = 11.84DD42 pKa = 3.7PRR44 pKa = 11.84EE45 pKa = 3.84EE46 pKa = 4.28EE47 pKa = 3.99PPHH50 pKa = 8.23AEE52 pKa = 3.92VEE54 pKa = 4.22KK55 pKa = 11.05SSWCGWSIRR64 pKa = 11.84ILHH67 pKa = 6.63GWMQWGPDD75 pKa = 3.69GMPFTHH81 pKa = 6.75IGTRR85 pKa = 11.84RR86 pKa = 11.84SAEE89 pKa = 3.44ARR91 pKa = 11.84ARR93 pKa = 11.84RR94 pKa = 11.84LLARR98 pKa = 11.84YY99 pKa = 5.44TRR101 pKa = 11.84KK102 pKa = 10.0YY103 pKa = 9.55EE104 pKa = 3.91RR105 pKa = 11.84EE106 pKa = 3.83RR107 pKa = 11.84QAKK110 pKa = 8.23IDD112 pKa = 3.38KK113 pKa = 10.44RR114 pKa = 11.84RR115 pKa = 11.84IEE117 pKa = 4.1LVSPEE122 pKa = 3.98EE123 pKa = 3.64FHH125 pKa = 7.39RR126 pKa = 11.84GVEE129 pKa = 4.18HH130 pKa = 7.4RR131 pKa = 11.84RR132 pKa = 11.84EE133 pKa = 3.92TAQALADD140 pKa = 3.96LRR142 pKa = 11.84DD143 pKa = 3.72PDD145 pKa = 4.35GAWEE149 pKa = 4.25RR150 pKa = 11.84EE151 pKa = 4.17FAALEE156 pKa = 4.02APRR159 pKa = 11.84RR160 pKa = 11.84RR161 pKa = 11.84QWWQRR166 pKa = 3.09

Molecular weight: 19.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17872

45

949

188.1

20.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.908 ± 0.364

0.968 ± 0.133

6.356 ± 0.317

6.966 ± 0.354

2.177 ± 0.127

8.824 ± 0.35

2.143 ± 0.205

4.7 ± 0.246

2.311 ± 0.185

7.045 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.117

2.496 ± 0.137

6.485 ± 0.283

2.921 ± 0.174

7.638 ± 0.471

5.013 ± 0.268

7.666 ± 0.27

6.804 ± 0.253

1.925 ± 0.152

2.328 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski