Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus; Equid alphaherpesvirus 1

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P28990|ICP0_EHV1B E3 ubiquitin-protein ligase ICP0 OS=Equine herpesvirus 1 (strain Ab4p) OX=31520 GN=63 PE=1 SV=1
MM1 pKa = 8.19DD2 pKa = 4.32SPRR5 pKa = 11.84GISTATGDD13 pKa = 3.61AHH15 pKa = 7.96AEE17 pKa = 4.08AAVSPAAEE25 pKa = 3.94IQIKK29 pKa = 8.96TEE31 pKa = 4.09APDD34 pKa = 3.29VDD36 pKa = 4.13GPEE39 pKa = 4.08ATTEE43 pKa = 4.3CLDD46 pKa = 3.36HH47 pKa = 7.29TYY49 pKa = 8.85TQQTSGGDD57 pKa = 3.37GLDD60 pKa = 5.27AIDD63 pKa = 4.47TDD65 pKa = 4.64DD66 pKa = 4.45LLEE69 pKa = 4.23MVLTSEE75 pKa = 4.37NTEE78 pKa = 4.15SEE80 pKa = 3.98PGIPFALRR88 pKa = 11.84GNFICCRR95 pKa = 11.84DD96 pKa = 3.96DD97 pKa = 3.15NCRR100 pKa = 11.84ACRR103 pKa = 11.84EE104 pKa = 4.19LPFRR108 pKa = 11.84PSVIGFSRR116 pKa = 11.84DD117 pKa = 3.27PHH119 pKa = 7.61VSMALDD125 pKa = 3.42MTSGNWAYY133 pKa = 10.72VPRR136 pKa = 11.84VFPDD140 pKa = 3.36TPTAPWMANYY150 pKa = 9.09CIPDD154 pKa = 3.85LDD156 pKa = 3.84EE157 pKa = 5.0HH158 pKa = 7.86ADD160 pKa = 3.39

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P28955|LTP_EHV1B Large tegument protein deneddylase OS=Equine herpesvirus 1 (strain Ab4p) OX=31520 GN=24 PE=3 SV=1
MM1 pKa = 8.14DD2 pKa = 4.88SYY4 pKa = 11.57NYY6 pKa = 10.25RR7 pKa = 11.84DD8 pKa = 3.63FAVGGGLLQRR18 pKa = 11.84IRR20 pKa = 11.84LVVSGSLHH28 pKa = 6.28CGEE31 pKa = 5.5SDD33 pKa = 3.87ATLNDD38 pKa = 4.12PKK40 pKa = 10.44HH41 pKa = 6.21LPARR45 pKa = 11.84CVFQFSGPDD54 pKa = 3.47NNSVTFPIEE63 pKa = 3.78YY64 pKa = 10.35VLRR67 pKa = 11.84LMKK70 pKa = 10.45NWARR74 pKa = 11.84SQCDD78 pKa = 3.28PYY80 pKa = 11.06IRR82 pKa = 11.84IQNTGVSVLFQGFFFAPPNAPMASITSEE110 pKa = 4.06HH111 pKa = 6.54NNVILKK117 pKa = 8.3STHH120 pKa = 4.24TTGLALSGIEE130 pKa = 3.82RR131 pKa = 11.84VKK133 pKa = 10.89RR134 pKa = 11.84GGGLDD139 pKa = 3.83LRR141 pKa = 11.84PLQAMMQISCFTRR154 pKa = 11.84MPVVQLSFRR163 pKa = 11.84FMGPEE168 pKa = 3.87DD169 pKa = 3.47ASRR172 pKa = 11.84TQRR175 pKa = 11.84LLEE178 pKa = 4.12RR179 pKa = 11.84ATSFGAMEE187 pKa = 4.24LHH189 pKa = 6.4QKK191 pKa = 8.65RR192 pKa = 11.84TVDD195 pKa = 3.12SCDD198 pKa = 3.07RR199 pKa = 11.84SNGIVSPRR207 pKa = 11.84EE208 pKa = 3.51HH209 pKa = 6.6RR210 pKa = 11.84EE211 pKa = 3.39CRR213 pKa = 11.84EE214 pKa = 3.77RR215 pKa = 11.84QKK217 pKa = 10.84RR218 pKa = 11.84RR219 pKa = 11.84PTPKK223 pKa = 9.11RR224 pKa = 11.84CASEE228 pKa = 4.46VFASLASISSAFASEE243 pKa = 4.21RR244 pKa = 11.84VKK246 pKa = 10.71RR247 pKa = 11.84RR248 pKa = 11.84PVRR251 pKa = 11.84IAAAILAFVFVAVILAIATKK271 pKa = 10.57GRR273 pKa = 11.84LFF275 pKa = 3.8

Molecular weight: 30.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

1

76

40266

74

3421

529.8

58.0

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.986 ± 0.405

1.895 ± 0.161

5.29 ± 0.145

5.412 ± 0.136

3.663 ± 0.178

6.34 ± 0.195

2.148 ± 0.115

4.128 ± 0.137

3.204 ± 0.191

9.291 ± 0.225

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.148 ± 0.106

3.497 ± 0.137

6.561 ± 0.292

3.276 ± 0.126

6.83 ± 0.217

8.454 ± 0.283

6.713 ± 0.46

7.147 ± 0.202

0.998 ± 0.049

3.02 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski