Hydrogenophaga sp. A37

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Hydrogenophaga; unclassified Hydrogenophaga

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5133 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V3RXN7|A0A1V3RXN7_9BURK LysR family transcriptional regulator OS=Hydrogenophaga sp. A37 OX=1945864 GN=B0E41_17180 PE=3 SV=1
MM1 pKa = 7.32SAVADD6 pKa = 4.33NIQTDD11 pKa = 3.84MPAPLVFTDD20 pKa = 3.72SAAAKK25 pKa = 9.57VAEE28 pKa = 4.23LVAEE32 pKa = 4.46EE33 pKa = 4.73GNPDD37 pKa = 2.98LKK39 pKa = 11.13LRR41 pKa = 11.84VFVQGGGCSGFQYY54 pKa = 10.9GFTFDD59 pKa = 3.8EE60 pKa = 4.88VVNEE64 pKa = 5.15DD65 pKa = 3.97DD66 pKa = 3.41TTMTKK71 pKa = 10.66NGVSLLIDD79 pKa = 3.54AMSYY83 pKa = 10.32QYY85 pKa = 11.5LVGAEE90 pKa = 3.79IDD92 pKa = 3.77YY93 pKa = 11.37KK94 pKa = 11.13EE95 pKa = 4.6DD96 pKa = 3.47LEE98 pKa = 4.54GAQFVIKK105 pKa = 10.48NPNASTTCGCGSSFSVV121 pKa = 3.54

Molecular weight: 12.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V3SHR2|A0A1V3SHR2_9BURK Uncharacterized protein OS=Hydrogenophaga sp. A37 OX=1945864 GN=B0E41_02135 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 9.15IKK11 pKa = 10.36RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.98GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5133

0

5133

1643886

24

4690

320.3

34.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.43 ± 0.043

0.959 ± 0.011

5.228 ± 0.029

5.175 ± 0.028

3.532 ± 0.021

8.342 ± 0.031

2.458 ± 0.018

4.117 ± 0.021

3.232 ± 0.035

10.911 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.559 ± 0.014

2.653 ± 0.022

5.348 ± 0.027

4.171 ± 0.025

6.623 ± 0.036

5.421 ± 0.025

5.359 ± 0.034

7.792 ± 0.029

1.593 ± 0.016

2.096 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski