Aeromonas phage AsXd-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5H1G6|A0A2Z5H1G6_9CAUD Uncharacterized protein OS=Aeromonas phage AsXd-1 OX=2182324 PE=4 SV=1
MM1 pKa = 7.43NEE3 pKa = 3.75DD4 pKa = 4.87DD5 pKa = 5.48IYY7 pKa = 11.94ALLSPLAEE15 pKa = 3.94GRR17 pKa = 11.84VYY19 pKa = 10.54PYY21 pKa = 10.4VAPLGSDD28 pKa = 4.08GKK30 pKa = 10.04PSVSPPWIIFSIVDD44 pKa = 3.62DD45 pKa = 4.2VSADD49 pKa = 3.71VLCGQAEE56 pKa = 4.41SRR58 pKa = 11.84VSIQVDD64 pKa = 4.18VYY66 pKa = 10.09STTITEE72 pKa = 4.19SRR74 pKa = 11.84TLRR77 pKa = 11.84DD78 pKa = 3.12LALASLKK85 pKa = 10.07PLNPTEE91 pKa = 4.18VVKK94 pKa = 10.96IPGYY98 pKa = 9.77EE99 pKa = 3.66PDD101 pKa = 3.38YY102 pKa = 10.74RR103 pKa = 11.84LYY105 pKa = 10.87RR106 pKa = 11.84ATLDD110 pKa = 3.72FKK112 pKa = 11.21VIPP115 pKa = 4.36

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5H086|A0A2Z5H086_9CAUD Uncharacterized protein OS=Aeromonas phage AsXd-1 OX=2182324 PE=4 SV=1
MM1 pKa = 7.16TWQTLILNINAVACILITIRR21 pKa = 11.84LMFFRR26 pKa = 11.84KK27 pKa = 9.3RR28 pKa = 11.84SLRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84RR34 pKa = 11.84LMEE37 pKa = 3.72FLAYY41 pKa = 10.29GLILAPAFTAFRR53 pKa = 11.84IWHH56 pKa = 6.25GDD58 pKa = 3.57YY59 pKa = 11.1VQVDD63 pKa = 3.69YY64 pKa = 11.92GEE66 pKa = 4.79LVVNLVVCIAVWRR79 pKa = 11.84ARR81 pKa = 11.84GNIARR86 pKa = 11.84IAGEE90 pKa = 4.19STTT93 pKa = 4.05

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11937

36

1279

229.6

25.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.053 ± 0.551

1.039 ± 0.155

5.839 ± 0.238

6.023 ± 0.277

3.351 ± 0.206

7.891 ± 0.264

1.826 ± 0.257

5.596 ± 0.247

5.11 ± 0.286

7.841 ± 0.286

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.167

4.817 ± 0.216

3.753 ± 0.281

4.566 ± 0.357

6.074 ± 0.312

6.903 ± 0.263

5.671 ± 0.36

6.886 ± 0.279

1.55 ± 0.113

2.656 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski