Mycobacterium marinum (strain ATCC BAA-535 / M)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium marinum

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5418 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B2HN11|B2HN11_MYCMM PE-PGRS family protein OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=MMAR_5555 PE=4 SV=1
MM1 pKa = 7.59SYY3 pKa = 11.01VIATPDD9 pKa = 3.37MLAAAASDD17 pKa = 3.52LTNIGSTLGAANSAALARR35 pKa = 11.84TTGLLAAAEE44 pKa = 4.79DD45 pKa = 4.3EE46 pKa = 4.57VSTAIASLFSASAQEE61 pKa = 4.05YY62 pKa = 10.01QMVSTRR68 pKa = 11.84AAHH71 pKa = 5.78LHH73 pKa = 5.9AEE75 pKa = 4.74FVHH78 pKa = 6.04SLTRR82 pKa = 11.84AGAAYY87 pKa = 9.83AAAEE91 pKa = 4.21AVNASPLQTLEE102 pKa = 3.95QNVLAAINAPSQALTARR119 pKa = 11.84PLFGDD124 pKa = 4.35GADD127 pKa = 3.98GAPGTGQNGGDD138 pKa = 3.93GGWLIGNGGNGGSGAPGGNGGAGGSAGLWGRR169 pKa = 11.84GGNGGAGGTGVAGGAGGNGGSGGANGLLGGGWGPFGGASGGAGGAGGAGGIGVAGGLGGDD229 pKa = 4.35GGAGGAGGAGGANSQLLSLWGSAGAGGAGGIGGAGGAGTDD269 pKa = 4.12GSAGGIGADD278 pKa = 3.99GGAGGHH284 pKa = 6.27GGVGGAGGANNAWLGGAAGSGGTGGSGGTGGLGGAGGSGIGYY326 pKa = 7.74STGGGAGGAGGAGGDD341 pKa = 3.83AGTGGAAGATAFLGSAGVAGSAGNGGHH368 pKa = 6.67GGNGGSGADD377 pKa = 3.66GDD379 pKa = 3.97IGVAGSGAGADD390 pKa = 3.98GGNGFAGGTGGDD402 pKa = 3.91GGTGGNTTGGSNGSGGNAGHH422 pKa = 7.08GGNAGDD428 pKa = 4.06GAAGNTGVGFATKK441 pKa = 10.2GGNGGNGGIGGNAGTAGLAGTGGTGGTTGAVGNAGHH477 pKa = 6.97GGNGGNGADD486 pKa = 4.09GDD488 pKa = 4.0IGVAGIGAGADD499 pKa = 3.56GGNGFAGGTGGDD511 pKa = 3.91GGTGGNTTGGTNGSGGNAGHH531 pKa = 7.13GGNAGNGAAGNTGVGFATKK550 pKa = 10.2GGNGGNGGIGGNAGTAGLAGTGGTGGTTGAVGNAGHH586 pKa = 6.97GGNGGNGADD595 pKa = 4.09GDD597 pKa = 4.0IGVAGIGAGADD608 pKa = 3.56GGNGFAGGTGGDD620 pKa = 3.91GGTGGNTTGGTNGSGGNAGHH640 pKa = 7.13GGNAGNGAAGNTGVGFATKK659 pKa = 10.2GGNGGNGGIGGNAGTAGLAGTGGTGGTTGAVGNAGHH695 pKa = 6.97GGNGGNGADD704 pKa = 4.09GDD706 pKa = 4.0IGVAGIGAGADD717 pKa = 3.56GGNGFAGGTGGDD729 pKa = 3.91GGTGGNTTGGTNGSGGNAGHH749 pKa = 7.13GGNAGNGAAGNTGVGFATKK768 pKa = 10.2GGNGGNGGIGGNAGTAGLAGTGGTGGTTGAVGNAGHH804 pKa = 6.97GGNGGNGADD813 pKa = 4.09GDD815 pKa = 4.0IGVAGIGAGADD826 pKa = 3.56GGNGFAGGTGGDD838 pKa = 3.91GGTGGNTTGGTNGSGGNAGHH858 pKa = 7.13GGNAGNGADD867 pKa = 4.16GNVGDD872 pKa = 4.82YY873 pKa = 10.21YY874 pKa = 11.59VSGGTGGMGGDD885 pKa = 3.83GGDD888 pKa = 3.46AGTAGLAGTGGSGGTVGAYY907 pKa = 9.57SAGGAAGNGGTGGAGAQGAPAQFGSTGFAGGDD939 pKa = 3.31GGAGGDD945 pKa = 3.8GGTGGGGNGGTNGNGGHH962 pKa = 6.85GGTGGAGGQGGVGYY976 pKa = 9.23TDD978 pKa = 4.87DD979 pKa = 4.24GSNNATTGGDD989 pKa = 3.65GGDD992 pKa = 3.94GGQGGQGGVPGATGTGGTVGMAGNGGAGGAGGQGGDD1028 pKa = 3.43TTTSGNSAGFGGQGGQGGQGGAGLSGLTGGDD1059 pKa = 4.1GYY1061 pKa = 11.39DD1062 pKa = 3.8GGQGGAGGTGGAGGDD1077 pKa = 3.84AVSGAYY1083 pKa = 9.57NGHH1086 pKa = 6.59GGAGGDD1092 pKa = 4.02GGAGGQGGTGHH1103 pKa = 7.56ADD1105 pKa = 3.3DD1106 pKa = 5.27GSNNAGAGGNGGMGGQGGQGGGAGAGAGAGSGAGSAGTGGDD1147 pKa = 3.6GGTGGDD1153 pKa = 3.78GGATTTIGHH1162 pKa = 5.6TAGAGGVGGQGGDD1175 pKa = 3.76GGDD1178 pKa = 3.88GLADD1182 pKa = 3.64AASSGGKK1189 pKa = 9.99GGAGGLGGNGGDD1201 pKa = 3.83AVSGAYY1207 pKa = 9.75NGQGGMGGQGGTGGQGGDD1225 pKa = 4.05AGPEE1229 pKa = 4.09GNGGNGGNGGDD1240 pKa = 4.07GGTAGAAGTGGLGGTAGTAGAGGTGGGGGQGGDD1273 pKa = 3.27TTTAGHH1279 pKa = 5.39TAGWGGNGGYY1289 pKa = 10.11GGQGGGGAAGPAGGSGSDD1307 pKa = 3.77GGQGGQGGAGGTGGDD1322 pKa = 3.67AVAGATNGSGGTGGYY1337 pKa = 9.65GGQGGQGGAGGTDD1350 pKa = 3.2DD1351 pKa = 5.4GGDD1354 pKa = 3.41NAGTGGAGGTGGTGGAAGATGSGGTVDD1381 pKa = 4.38LAFPGTAGHH1390 pKa = 6.65GGDD1393 pKa = 4.05GGAGGGGGATTIVGHH1408 pKa = 5.14TAGTGGSGGQGGEE1421 pKa = 3.99GGAAADD1427 pKa = 3.82TGVPGAAGSTGGFGGTGGAGGAGGDD1452 pKa = 3.73AVAGATNGSGGAGGQGGAGAKK1473 pKa = 9.92GGQGYY1478 pKa = 10.67DD1479 pKa = 3.9DD1480 pKa = 5.81DD1481 pKa = 4.72GANNAGVGGNGGEE1494 pKa = 4.41GGTGGSGGAAGTGGDD1509 pKa = 3.95GGTAGAAGTGGTGGDD1524 pKa = 3.73GGKK1527 pKa = 10.64GGATTISGHH1536 pKa = 5.23HH1537 pKa = 6.56AGYY1540 pKa = 10.29GGNAGGGGQGADD1552 pKa = 3.71GAAGPAGGNGYY1563 pKa = 10.62DD1564 pKa = 4.03GGQGGAGGTGGQGGDD1579 pKa = 3.37AVAGATNGYY1588 pKa = 9.06GGQGGIGGTGGQGGGGGADD1607 pKa = 3.93DD1608 pKa = 5.2GSDD1611 pKa = 3.21NAGAGGSGGAGGLGGQSGAASTGGIVGTGGTGFAGDD1647 pKa = 4.43GGDD1650 pKa = 4.24GGAGGQGGATAVSGHH1665 pKa = 5.23TAGAGGTGGTGGTGGDD1681 pKa = 4.51GIDD1684 pKa = 4.33GPADD1688 pKa = 3.73RR1689 pKa = 11.84PGGRR1693 pKa = 11.84GGQGGHH1699 pKa = 6.5GGDD1702 pKa = 3.75GGSGGSAVAGATNGSGGAGGAGGTGGQGGNGYY1734 pKa = 10.56DD1735 pKa = 4.97DD1736 pKa = 5.59DD1737 pKa = 6.57GSDD1740 pKa = 3.29AAGAGGAGGDD1750 pKa = 3.72GGVGGTAGAVGSGGSGGTAGTAGTGGDD1777 pKa = 3.95GGDD1780 pKa = 3.74GGKK1783 pKa = 10.65GGATTTIGSSAGSGGDD1799 pKa = 3.41GGNGGLGGAGAIGPAGADD1817 pKa = 3.45GSTGGQGGAGGQGGDD1832 pKa = 3.98GGDD1835 pKa = 3.56AVAGATNGLGGNGGDD1850 pKa = 4.29GGSGGQGGAGGVDD1863 pKa = 3.6DD1864 pKa = 5.46GGGAGTGGQGGEE1876 pKa = 4.31GGQAGDD1882 pKa = 4.15GGVSGSGGLFGGGGDD1897 pKa = 4.27GGHH1900 pKa = 6.93GGQGGLGGTGGDD1912 pKa = 3.59SALGTGGDD1920 pKa = 4.15GGQGGQGGTGGTGGYY1935 pKa = 10.1GAFEE1939 pKa = 4.57GGFGGDD1945 pKa = 3.77GGDD1948 pKa = 3.79GGTGGTGGVGQSGLPGTGGTIGIAGVPGSLPSGSTGGAGGDD1989 pKa = 3.94GGDD1992 pKa = 4.58GGDD1995 pKa = 4.59GGPGSPP2001 pKa = 4.79

Molecular weight: 160.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B2HNW0|B2HNW0_MYCMM PPE family protein PPE3 OS=Mycobacterium marinum (strain ATCC BAA-535 / M) OX=216594 GN=MMAR_0538 PE=3 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVTGRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.63GRR42 pKa = 11.84RR43 pKa = 11.84ALTAA47 pKa = 3.88

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5418

0

5418

1984709

32

9858

366.3

38.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.223 ± 0.053

0.821 ± 0.012

5.812 ± 0.029

4.773 ± 0.036

3.017 ± 0.022

10.606 ± 0.176

2.228 ± 0.035

4.277 ± 0.024

1.941 ± 0.024

9.696 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.98 ± 0.017

2.585 ± 0.041

5.758 ± 0.046

3.235 ± 0.018

6.713 ± 0.051

5.665 ± 0.027

5.992 ± 0.056

8.127 ± 0.044

1.457 ± 0.014

2.095 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski