Facklamia languida CCUG 37842

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Facklamia; Facklamia languida

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H3NGR0|H3NGR0_9LACT SSD domain-containing protein OS=Facklamia languida CCUG 37842 OX=883113 GN=HMPREF9708_00049 PE=4 SV=1
MM1 pKa = 7.56NKK3 pKa = 7.52MTFLKK8 pKa = 10.58KK9 pKa = 9.61LTLAALFANFLCPPLTHH26 pKa = 6.52AQEE29 pKa = 4.48DD30 pKa = 4.11QATEE34 pKa = 4.07DD35 pKa = 3.37TSVTNEE41 pKa = 3.85EE42 pKa = 4.06GLDD45 pKa = 4.0LIEE48 pKa = 5.65SEE50 pKa = 5.35ASLEE54 pKa = 4.11DD55 pKa = 3.82LMDD58 pKa = 6.55DD59 pKa = 5.08IIQANQSVQSVVQEE73 pKa = 4.02IEE75 pKa = 4.05LDD77 pKa = 3.77SKK79 pKa = 11.22VSQEE83 pKa = 4.22GEE85 pKa = 3.53EE86 pKa = 4.48DD87 pKa = 3.78LFNQKK92 pKa = 10.1AVMEE96 pKa = 5.06AIYY99 pKa = 10.72GEE101 pKa = 4.25EE102 pKa = 4.24GQVDD106 pKa = 4.05GAYY109 pKa = 9.47IYY111 pKa = 11.06SLYY114 pKa = 10.28DD115 pKa = 3.2QNGEE119 pKa = 3.97QMIRR123 pKa = 11.84EE124 pKa = 3.98QLMPTNEE131 pKa = 3.7KK132 pKa = 9.85TIYY135 pKa = 9.71TRR137 pKa = 11.84FNEE140 pKa = 4.33EE141 pKa = 3.44EE142 pKa = 4.15WEE144 pKa = 4.12KK145 pKa = 11.53DD146 pKa = 3.02STEE149 pKa = 4.07YY150 pKa = 10.7TDD152 pKa = 3.15QDD154 pKa = 3.96QYY156 pKa = 12.01FLTPNYY162 pKa = 9.94HH163 pKa = 6.8HH164 pKa = 7.34LLADD168 pKa = 3.95LLEE171 pKa = 4.54LEE173 pKa = 4.78SYY175 pKa = 8.02YY176 pKa = 10.85TLYY179 pKa = 11.04EE180 pKa = 4.29DD181 pKa = 3.84ANSYY185 pKa = 10.72ILITKK190 pKa = 9.82DD191 pKa = 2.71QDD193 pKa = 3.02ADD195 pKa = 4.0LFHH198 pKa = 6.86VLQPHH203 pKa = 7.03FDD205 pKa = 3.66LQLTGMEE212 pKa = 4.61PEE214 pKa = 4.69AFHH217 pKa = 6.67QGVFLSINKK226 pKa = 7.97DD227 pKa = 3.15TLLFEE232 pKa = 5.79DD233 pKa = 4.67LMFVMTYY240 pKa = 11.1DD241 pKa = 4.42DD242 pKa = 4.69GSSSLEE248 pKa = 3.85MSAVAIMSEE257 pKa = 3.87WDD259 pKa = 3.41KK260 pKa = 11.79LNASDD265 pKa = 4.11YY266 pKa = 11.57QMDD269 pKa = 3.51QSEE272 pKa = 4.5PGQEE276 pKa = 4.29EE277 pKa = 3.89IDD279 pKa = 3.77QEE281 pKa = 4.39EE282 pKa = 4.37VDD284 pKa = 3.8QEE286 pKa = 4.16EE287 pKa = 4.56SSLEE291 pKa = 3.93EE292 pKa = 3.96TDD294 pKa = 4.63QEE296 pKa = 4.46EE297 pKa = 4.51ADD299 pKa = 3.63QEE301 pKa = 4.2ASQEE305 pKa = 4.22EE306 pKa = 4.78TSQEE310 pKa = 3.78

Molecular weight: 35.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H3NIK5|H3NIK5_9LACT Alpha alpha-phosphotrehalase OS=Facklamia languida CCUG 37842 OX=883113 GN=HMPREF9708_00694 PE=4 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.96RR4 pKa = 11.84TYY6 pKa = 9.59QPKK9 pKa = 9.46KK10 pKa = 7.41RR11 pKa = 11.84HH12 pKa = 5.45RR13 pKa = 11.84SKK15 pKa = 10.57VHH17 pKa = 5.58GFRR20 pKa = 11.84ARR22 pKa = 11.84MSTKK26 pKa = 9.41NGRR29 pKa = 11.84RR30 pKa = 11.84VLSKK34 pKa = 10.63RR35 pKa = 11.84RR36 pKa = 11.84LKK38 pKa = 10.67GRR40 pKa = 11.84KK41 pKa = 8.83KK42 pKa = 10.59LSAA45 pKa = 3.95

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1527

0

1527

486735

31

3137

318.8

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.66 ± 0.07

0.599 ± 0.017

6.121 ± 0.048

6.872 ± 0.084

4.089 ± 0.049

6.45 ± 0.065

2.198 ± 0.035

7.211 ± 0.06

5.391 ± 0.058

10.342 ± 0.096

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.573 ± 0.026

3.95 ± 0.037

3.869 ± 0.042

5.698 ± 0.076

4.32 ± 0.051

5.823 ± 0.045

5.293 ± 0.072

6.782 ± 0.048

0.921 ± 0.023

3.837 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski