Avon-Heathcote Estuary associated circular virus 25

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBT8|A0A0C5IBT8_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 25 OX=1618249 PE=4 SV=1
MM1 pKa = 7.25ARR3 pKa = 11.84EE4 pKa = 3.8NRR6 pKa = 11.84FRR8 pKa = 11.84LQCRR12 pKa = 11.84RR13 pKa = 11.84LFLTYY18 pKa = 7.92PHH20 pKa = 6.75CTASIQEE27 pKa = 4.42VYY29 pKa = 11.2DD30 pKa = 4.68LIHH33 pKa = 6.51SKK35 pKa = 11.04KK36 pKa = 9.28EE37 pKa = 3.5IKK39 pKa = 9.62FARR42 pKa = 11.84ICIEE46 pKa = 3.85PHH48 pKa = 6.42SDD50 pKa = 3.29GTPHH54 pKa = 5.88IHH56 pKa = 6.34AAVMFAKK63 pKa = 10.14KK64 pKa = 10.77VNICNARR71 pKa = 11.84LFDD74 pKa = 5.69INDD77 pKa = 3.31HH78 pKa = 6.08HH79 pKa = 8.36CNMEE83 pKa = 4.77SIKK86 pKa = 10.61NWPATLNYY94 pKa = 9.39CKK96 pKa = 10.53KK97 pKa = 10.2GDD99 pKa = 3.42NWEE102 pKa = 5.1DD103 pKa = 3.43FDD105 pKa = 6.37NDD107 pKa = 4.47DD108 pKa = 6.22DD109 pKa = 5.43PDD111 pKa = 4.33QPMQDD116 pKa = 3.31NLFEE120 pKa = 4.46LAEE123 pKa = 4.28TMPSNDD129 pKa = 2.35FWEE132 pKa = 4.35YY133 pKa = 11.06CRR135 pKa = 11.84LKK137 pKa = 10.52KK138 pKa = 10.95VPFAYY143 pKa = 10.2ASNAMRR149 pKa = 11.84KK150 pKa = 8.2KK151 pKa = 10.64KK152 pKa = 10.93SMFTITDD159 pKa = 3.44STPYY163 pKa = 10.75GIIRR167 pKa = 11.84EE168 pKa = 4.13DD169 pKa = 3.13LGAYY173 pKa = 7.83TITEE177 pKa = 4.4DD178 pKa = 4.86SPATLASTVLVGPSGIGKK196 pKa = 7.24TSWAIRR202 pKa = 11.84EE203 pKa = 4.23SEE205 pKa = 4.29KK206 pKa = 10.73PSLLVTHH213 pKa = 6.75MDD215 pKa = 3.95ALRR218 pKa = 11.84KK219 pKa = 10.13LNGSHH224 pKa = 7.06KK225 pKa = 10.75SIIFDD230 pKa = 3.66DD231 pKa = 3.8MSFTHH236 pKa = 7.1IPRR239 pKa = 11.84EE240 pKa = 4.27AQIHH244 pKa = 5.26LVDD247 pKa = 4.17RR248 pKa = 11.84FQPRR252 pKa = 11.84QVHH255 pKa = 4.64VRR257 pKa = 11.84YY258 pKa = 8.23GTADD262 pKa = 3.13IPAGIQKK269 pKa = 9.79IFTANQYY276 pKa = 10.75PFVADD281 pKa = 3.47PAIDD285 pKa = 3.03RR286 pKa = 11.84RR287 pKa = 11.84VHH289 pKa = 6.12NKK291 pKa = 9.56NLYY294 pKa = 9.31

Molecular weight: 33.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBT8|A0A0C5IBT8_9CIRC Putative capsid protein OS=Avon-Heathcote Estuary associated circular virus 25 OX=1618249 PE=4 SV=1
MM1 pKa = 7.69SYY3 pKa = 10.58NGRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84VARR11 pKa = 11.84SSRR14 pKa = 11.84YY15 pKa = 7.48NARR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 10.19SPYY23 pKa = 10.15SPYY26 pKa = 10.91SLRR29 pKa = 11.84SAVRR33 pKa = 11.84VGRR36 pKa = 11.84YY37 pKa = 8.57LGNYY41 pKa = 8.01ARR43 pKa = 11.84KK44 pKa = 10.01SFNNWRR50 pKa = 11.84SKK52 pKa = 9.87TSAKK56 pKa = 9.47KK57 pKa = 8.88QRR59 pKa = 11.84RR60 pKa = 11.84NIGNKK65 pKa = 9.47IISASNGIGYY75 pKa = 10.1SYY77 pKa = 10.68FRR79 pKa = 11.84EE80 pKa = 4.0NRR82 pKa = 11.84KK83 pKa = 9.88CYY85 pKa = 9.69PNVRR89 pKa = 11.84NMLKK93 pKa = 10.51AGTLSVYY100 pKa = 10.38KK101 pKa = 10.88YY102 pKa = 10.94VDD104 pKa = 3.42TKK106 pKa = 10.89RR107 pKa = 11.84LEE109 pKa = 3.94WDD111 pKa = 3.19AGSQGVLNVATTFGVQQIQNVMNNIGASANGDD143 pKa = 3.28DD144 pKa = 4.09TTRR147 pKa = 11.84MVVKK151 pKa = 10.48GVTGSVNISNMSNANVLITLYY172 pKa = 10.89DD173 pKa = 3.5VVARR177 pKa = 11.84TDD179 pKa = 3.8SNTSSAWANPLNAWGNGLEE198 pKa = 4.22DD199 pKa = 3.9SEE201 pKa = 5.04EE202 pKa = 4.51GGSVAAGRR210 pKa = 11.84NNIGATPFQSVMFCRR225 pKa = 11.84KK226 pKa = 8.25YY227 pKa = 10.89RR228 pKa = 11.84VMKK231 pKa = 8.15VTKK234 pKa = 9.56IYY236 pKa = 9.7MEE238 pKa = 4.55AGRR241 pKa = 11.84SHH243 pKa = 5.78IHH245 pKa = 5.39YY246 pKa = 9.96IKK248 pKa = 10.83RR249 pKa = 11.84NMNSVLNNSIMEE261 pKa = 4.32TNEE264 pKa = 3.48TNLANKK270 pKa = 7.55THH272 pKa = 6.23YY273 pKa = 10.16CFAVARR279 pKa = 11.84GFPVNDD285 pKa = 3.19ATTDD289 pKa = 3.42TNIALGSGALDD300 pKa = 3.39FVFTEE305 pKa = 5.61TYY307 pKa = 8.55QQVWPQYY314 pKa = 9.03ALKK317 pKa = 10.1RR318 pKa = 11.84YY319 pKa = 9.18YY320 pKa = 11.31YY321 pKa = 10.61NDD323 pKa = 3.18QQQAITSEE331 pKa = 3.97KK332 pKa = 10.63LMNAEE337 pKa = 4.01TGEE340 pKa = 4.03GDD342 pKa = 3.57VYY344 pKa = 11.54EE345 pKa = 4.43EE346 pKa = 4.31VV347 pKa = 3.4

Molecular weight: 38.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

641

294

347

320.5

36.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.112 ± 0.157

1.56 ± 0.444

5.46 ± 1.279

4.368 ± 0.213

3.744 ± 0.735

5.616 ± 1.199

2.496 ± 1.043

5.928 ± 1.026

5.616 ± 0.458

5.304 ± 0.443

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.032

8.268 ± 1.53

3.744 ± 1.104

3.276 ± 0.116

7.02 ± 0.486

7.332 ± 0.839

6.24 ± 0.248

6.24 ± 1.169

1.404 ± 0.024

5.148 ± 0.946
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski