Peptostreptococcus sp. D1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptostreptococcaceae; Peptostreptococcus; unclassified Peptostreptococcus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2116 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2BDG5|A0A1I2BDG5_9FIRM Phosphoribosylglycinamide formyltransferase OS=Peptostreptococcus sp. D1 OX=72304 GN=purN PE=3 SV=1
MM1 pKa = 7.93EE2 pKa = 5.45YY3 pKa = 11.26NLFDD7 pKa = 5.14YY8 pKa = 9.79ITDD11 pKa = 3.78EE12 pKa = 4.32NDD14 pKa = 2.99NSIDD18 pKa = 3.31NYY20 pKa = 9.97IRR22 pKa = 11.84KK23 pKa = 7.33TVIEE27 pKa = 4.29KK28 pKa = 10.54DD29 pKa = 2.93ISITVLSCALNISDD43 pKa = 6.11KK44 pKa = 11.27EE45 pKa = 4.02MLDD48 pKa = 3.27ILISQDD54 pKa = 2.94ILEE57 pKa = 5.22KK58 pKa = 10.3LDD60 pKa = 3.3CMQYY64 pKa = 11.04GEE66 pKa = 4.54FLRR69 pKa = 11.84VLEE72 pKa = 4.52IYY74 pKa = 10.33EE75 pKa = 4.04LL76 pKa = 3.92

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2CIN7|A0A1I2CIN7_9FIRM YidC/Oxa1 family membrane protein insertase OS=Peptostreptococcus sp. D1 OX=72304 GN=SAMN02910278_01430 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 8.57KK9 pKa = 7.61RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.25RR14 pKa = 11.84EE15 pKa = 3.66HH16 pKa = 6.29GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 9.11TSNGRR28 pKa = 11.84NVLKK32 pKa = 10.51RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.9GRR39 pKa = 11.84NRR41 pKa = 11.84LTHH44 pKa = 6.18

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2116

0

2116

665628

39

3941

314.6

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.628 ± 0.056

1.101 ± 0.023

6.162 ± 0.04

7.426 ± 0.061

4.387 ± 0.039

6.431 ± 0.052

1.309 ± 0.021

9.529 ± 0.064

8.954 ± 0.053

8.539 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.027

6.066 ± 0.055

2.678 ± 0.03

2.27 ± 0.029

3.833 ± 0.036

6.863 ± 0.051

4.7 ± 0.047

6.647 ± 0.047

0.642 ± 0.018

4.122 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski