Wenzhou tombus-like virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KH75|A0A1L3KH75_9VIRU Tombus_P33 domain-containing protein OS=Wenzhou tombus-like virus 5 OX=1923675 PE=4 SV=1
MM1 pKa = 7.42YY2 pKa = 9.45YY3 pKa = 10.43CKK5 pKa = 10.72VGDD8 pKa = 4.0TFLPAPEE15 pKa = 4.94PDD17 pKa = 3.69VNHH20 pKa = 5.61VFAGLRR26 pKa = 11.84EE27 pKa = 4.04FRR29 pKa = 11.84SKK31 pKa = 10.46LVHH34 pKa = 6.43RR35 pKa = 11.84MVSTPYY41 pKa = 9.93TIEE44 pKa = 4.14EE45 pKa = 4.11VVDD48 pKa = 3.72TYY50 pKa = 11.23KK51 pKa = 10.8GRR53 pKa = 11.84KK54 pKa = 6.26RR55 pKa = 11.84TIYY58 pKa = 9.56QQASEE63 pKa = 4.42SLNNTGLKK71 pKa = 9.74RR72 pKa = 11.84GHH74 pKa = 7.14AISDD78 pKa = 3.78CFVKK82 pKa = 10.71VEE84 pKa = 4.55KK85 pKa = 10.96GNTSKK90 pKa = 10.98APRR93 pKa = 11.84CIQPRR98 pKa = 11.84RR99 pKa = 11.84PEE101 pKa = 3.95YY102 pKa = 10.84NLVLGKK108 pKa = 9.93YY109 pKa = 8.75IKK111 pKa = 10.39KK112 pKa = 9.67IEE114 pKa = 3.69HH115 pKa = 5.29RR116 pKa = 11.84MYY118 pKa = 10.77RR119 pKa = 11.84RR120 pKa = 11.84IAKK123 pKa = 9.75IYY125 pKa = 9.84GDD127 pKa = 4.37GPTVMKK133 pKa = 10.49GYY135 pKa = 8.07TVQDD139 pKa = 3.23VARR142 pKa = 11.84IMRR145 pKa = 11.84GKK147 pKa = 9.03WDD149 pKa = 3.7SFSDD153 pKa = 4.03PVAVGLDD160 pKa = 3.27AVKK163 pKa = 10.78FDD165 pKa = 3.85MHH167 pKa = 7.39VSAAMLEE174 pKa = 4.47WEE176 pKa = 4.03HH177 pKa = 7.6SIYY180 pKa = 10.68KK181 pKa = 10.44AVTTDD186 pKa = 2.97TRR188 pKa = 11.84EE189 pKa = 4.07LNRR192 pKa = 11.84LLRR195 pKa = 11.84WQMFNKK201 pKa = 9.91GRR203 pKa = 11.84GRR205 pKa = 11.84CVDD208 pKa = 3.19GKK210 pKa = 9.48LTYY213 pKa = 8.37TVKK216 pKa = 10.67GKK218 pKa = 10.49RR219 pKa = 11.84FSGDD223 pKa = 2.78MNTALGNCIIMCGLVYY239 pKa = 10.1TYY241 pKa = 10.97LKK243 pKa = 9.93EE244 pKa = 4.27KK245 pKa = 10.24GMHH248 pKa = 6.21GKK250 pKa = 9.54LVNNGDD256 pKa = 3.76DD257 pKa = 3.26CVVFMEE263 pKa = 4.94RR264 pKa = 11.84GDD266 pKa = 3.72LEE268 pKa = 4.71GFVEE272 pKa = 6.34GLDD275 pKa = 3.2EE276 pKa = 4.08WFYY279 pKa = 11.51SYY281 pKa = 10.84GFRR284 pKa = 11.84MTSEE288 pKa = 4.04EE289 pKa = 4.08PVFDD293 pKa = 3.97FEE295 pKa = 6.25KK296 pKa = 10.69IEE298 pKa = 4.15FCQMRR303 pKa = 11.84PILGPNGWVMVRR315 pKa = 11.84NIKK318 pKa = 10.12AALVKK323 pKa = 10.71DD324 pKa = 4.1CLCTIDD330 pKa = 5.07LDD332 pKa = 4.37GVSARR337 pKa = 11.84KK338 pKa = 8.87WMYY341 pKa = 11.05AVGEE345 pKa = 4.42CGLALTSGIPIMQEE359 pKa = 3.89FYY361 pKa = 10.92LALMRR366 pKa = 11.84NGDD369 pKa = 3.66SSSNITQSLQFQSSGLRR386 pKa = 11.84HH387 pKa = 5.39MRR389 pKa = 11.84GEE391 pKa = 4.27LDD393 pKa = 3.34AKK395 pKa = 10.78ASAVSAEE402 pKa = 4.03ARR404 pKa = 11.84RR405 pKa = 11.84SVYY408 pKa = 9.55VAWGITPDD416 pKa = 3.47EE417 pKa = 4.05QVVLEE422 pKa = 3.96EE423 pKa = 4.93HH424 pKa = 5.66FRR426 pKa = 11.84SWVFDD431 pKa = 3.84PSPGDD436 pKa = 3.14IHH438 pKa = 7.74VSQPPIFHH446 pKa = 7.16GISLSWW452 pKa = 3.33

Molecular weight: 51.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGX3|A0A1L3KGX3_9VIRU RNA-directed RNA polymerase OS=Wenzhou tombus-like virus 5 OX=1923675 PE=4 SV=1
MM1 pKa = 6.64GTAAVTSHH9 pKa = 6.33SLYY12 pKa = 10.79NSNLLGCATCAGSSTRR28 pKa = 11.84KK29 pKa = 9.23LAPLVQRR36 pKa = 11.84RR37 pKa = 11.84VGAFMWPGVSRR48 pKa = 11.84LMSRR52 pKa = 11.84LCWRR56 pKa = 11.84STSVAGCLTLPPVIFTYY73 pKa = 10.85HH74 pKa = 6.03NLPYY78 pKa = 10.51FMALAYY84 pKa = 9.82HH85 pKa = 6.72GNYY88 pKa = 9.29VGPGWSAGKK97 pKa = 9.35YY98 pKa = 7.32QSSVEE103 pKa = 4.09SDD105 pKa = 3.6VLPVDD110 pKa = 4.12EE111 pKa = 5.96FDD113 pKa = 3.77ATGKK117 pKa = 10.11RR118 pKa = 11.84HH119 pKa = 6.01DD120 pKa = 3.64AVYY123 pKa = 10.97ARR125 pKa = 11.84GGDD128 pKa = 3.37RR129 pKa = 11.84TRR131 pKa = 11.84ADD133 pKa = 3.48FEE135 pKa = 4.09FAYY138 pKa = 10.83DD139 pKa = 4.45NLTTLNPKK147 pKa = 9.78RR148 pKa = 11.84WLAGALVGAQGLCRR162 pKa = 11.84FATGLNGITEE172 pKa = 4.35EE173 pKa = 4.62AEE175 pKa = 4.26GSQRR179 pKa = 11.84GVTKK183 pKa = 10.64HH184 pKa = 5.96SSQGLDD190 pKa = 2.85KK191 pKa = 10.7DD192 pKa = 4.22YY193 pKa = 11.26PPLSIDD199 pKa = 3.17NMKK202 pKa = 10.66NKK204 pKa = 9.97RR205 pKa = 11.84NRR207 pKa = 11.84IFAGDD212 pKa = 3.76SSHH215 pKa = 7.75LVMDD219 pKa = 4.06SNGRR223 pKa = 11.84RR224 pKa = 11.84EE225 pKa = 3.91VEE227 pKa = 4.6RR228 pKa = 11.84IDD230 pKa = 3.59KK231 pKa = 10.66QITKK235 pKa = 9.9SYY237 pKa = 10.45QKK239 pKa = 10.41RR240 pKa = 11.84KK241 pKa = 8.7PLKK244 pKa = 10.14QRR246 pKa = 11.84VQQAFSPLKK255 pKa = 7.71TTAAPVSIGTTLVAVPPQTVKK276 pKa = 10.06TGNGVCIAGRR286 pKa = 11.84EE287 pKa = 4.5FITQVYY293 pKa = 7.28GTNNDD298 pKa = 3.39SFQVAGLAPVHH309 pKa = 6.13PAYY312 pKa = 10.86YY313 pKa = 9.45PASTMGNISRR323 pKa = 11.84SYY325 pKa = 9.06QYY327 pKa = 11.13YY328 pKa = 9.13RR329 pKa = 11.84FRR331 pKa = 11.84KK332 pKa = 8.73IVAHH336 pKa = 6.25FVTRR340 pKa = 11.84QPTSVTGEE348 pKa = 3.53IVMCYY353 pKa = 9.79SKK355 pKa = 11.2NILDD359 pKa = 4.02PPEE362 pKa = 5.33NGDD365 pKa = 3.5SGSFLPRR372 pKa = 11.84AMTRR376 pKa = 11.84GNAVIGPLWQNHH388 pKa = 4.7SVEE391 pKa = 4.35IPLDD395 pKa = 3.42GTFRR399 pKa = 11.84LVDD402 pKa = 3.62AFNAGTFADD411 pKa = 3.9NVNGEE416 pKa = 4.4VQVYY420 pKa = 7.21TQTNVDD426 pKa = 3.9DD427 pKa = 3.93TCGYY431 pKa = 10.05LVWDD435 pKa = 3.89YY436 pKa = 11.77VLEE439 pKa = 4.35FSNTMFTPHH448 pKa = 6.09STSLPLVSGPGTKK461 pKa = 8.94YY462 pKa = 8.7TVSVAASAANDD473 pKa = 3.84ALIGTSSLITAYY485 pKa = 9.82PNQTVWSLALDD496 pKa = 4.26LSASSAGTGATLANLVNVFANYY518 pKa = 7.62HH519 pKa = 4.68TTTTALSQAGTNLPLVDD536 pKa = 3.92GQRR539 pKa = 11.84IFAMVVGGVLYY550 pKa = 9.8FYY552 pKa = 10.97SSYY555 pKa = 9.73EE556 pKa = 3.78AAVAGMGSAQLYY568 pKa = 9.81IRR570 pKa = 11.84TATTTASTLIFNGYY584 pKa = 7.97LVRR587 pKa = 11.84LGPVEE592 pKa = 3.92LTAANN597 pKa = 3.62

Molecular weight: 64.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1266

217

597

422.0

46.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.847 ± 1.456

2.054 ± 0.476

4.581 ± 0.509

4.581 ± 1.211

3.949 ± 0.223

7.978 ± 0.775

1.975 ± 0.204

3.87 ± 0.52

4.423 ± 0.915

7.741 ± 0.398

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.923 ± 0.617

5.134 ± 0.905

4.423 ± 0.383

3.397 ± 0.318

6.319 ± 0.763

6.714 ± 1.122

6.556 ± 1.577

8.847 ± 0.575

1.501 ± 0.229

4.186 ± 0.418
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski