Eubacterium sp. AF36-5BH

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Eubacterium; unclassified Eubacterium

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A373LDY6|A0A373LDY6_9FIRM MATE family efflux transporter OS=Eubacterium sp. AF36-5BH OX=2293108 GN=DW006_10730 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.53KK3 pKa = 8.95MNKK6 pKa = 8.98YY7 pKa = 9.07MKK9 pKa = 8.39TTIITVIILALFTIAQRR26 pKa = 11.84DD27 pKa = 3.83KK28 pKa = 10.53IIGYY32 pKa = 7.59FEE34 pKa = 4.43PKK36 pKa = 10.0CIEE39 pKa = 4.04EE40 pKa = 4.64GCTEE44 pKa = 4.03KK45 pKa = 10.89VVEE48 pKa = 4.19GTNYY52 pKa = 9.79CRR54 pKa = 11.84WHH56 pKa = 7.1LISDD60 pKa = 3.97DD61 pKa = 3.65NDD63 pKa = 3.55YY64 pKa = 11.57SDD66 pKa = 4.53TYY68 pKa = 11.28HH69 pKa = 6.79SGTVTTTTSAYY80 pKa = 10.76SDD82 pKa = 3.57NTDD85 pKa = 3.22GSHH88 pKa = 7.34SNNDD92 pKa = 3.13YY93 pKa = 11.63NNYY96 pKa = 10.19NNYY99 pKa = 10.24NNYY102 pKa = 10.16NSDD105 pKa = 3.74DD106 pKa = 3.88NEE108 pKa = 4.04YY109 pKa = 11.17DD110 pKa = 3.73DD111 pKa = 6.41DD112 pKa = 4.99SDD114 pKa = 4.05YY115 pKa = 11.18TQEE118 pKa = 4.03TTSDD122 pKa = 3.17NKK124 pKa = 9.83VYY126 pKa = 10.31KK127 pKa = 7.95YY128 pKa = 10.38HH129 pKa = 7.38KK130 pKa = 9.71RR131 pKa = 11.84IQYY134 pKa = 10.27DD135 pKa = 3.47SSEE138 pKa = 4.03DD139 pKa = 3.91AYY141 pKa = 11.2DD142 pKa = 4.55DD143 pKa = 5.03GYY145 pKa = 11.59DD146 pKa = 5.04DD147 pKa = 5.53IYY149 pKa = 11.68DD150 pKa = 4.48DD151 pKa = 5.02GDD153 pKa = 3.61YY154 pKa = 11.01DD155 pKa = 4.36YY156 pKa = 11.66DD157 pKa = 5.32RR158 pKa = 11.84YY159 pKa = 11.67DD160 pKa = 3.27NDD162 pKa = 2.88EE163 pKa = 4.8DD164 pKa = 4.01YY165 pKa = 11.83AEE167 pKa = 4.58GVDD170 pKa = 4.54DD171 pKa = 6.44AMAEE175 pKa = 4.13IDD177 pKa = 3.38WW178 pKa = 4.18

Molecular weight: 20.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A373LIA3|A0A373LIA3_9FIRM ATP-binding protein OS=Eubacterium sp. AF36-5BH OX=2293108 GN=DW006_09715 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 9.97RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.72VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.13GRR39 pKa = 11.84KK40 pKa = 8.85KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2295

0

2295

785904

25

7972

342.4

38.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.199 ± 0.063

1.354 ± 0.024

5.863 ± 0.065

7.263 ± 0.063

4.015 ± 0.036

6.583 ± 0.048

1.435 ± 0.021

8.402 ± 0.061

9.228 ± 0.063

7.825 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.851 ± 0.034

5.964 ± 0.048

2.734 ± 0.028

2.707 ± 0.028

3.367 ± 0.039

5.959 ± 0.043

5.782 ± 0.069

7.033 ± 0.043

0.841 ± 0.016

4.595 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski