Drosophila mojavensis (Fruit fly)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18165 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4K838|B4K838_DROMO Uncharacterized protein OS=Drosophila mojavensis OX=7230 GN=Dmoj\GI22809 PE=4 SV=2
MM1 pKa = 7.82IIDD4 pKa = 5.13DD5 pKa = 4.07NSLDD9 pKa = 3.66QVVAPTLVSANAFKK23 pKa = 11.24YY24 pKa = 10.68SMPLHH29 pKa = 6.43CHH31 pKa = 5.27TATASVLRR39 pKa = 11.84NYY41 pKa = 9.94RR42 pKa = 11.84HH43 pKa = 6.08NPLISGTNLQLSPVFSSSEE62 pKa = 3.88AASPLAGDD70 pKa = 4.48GDD72 pKa = 4.19ASSVASLDD80 pKa = 3.92DD81 pKa = 3.81SMPPGLTACDD91 pKa = 3.56TDD93 pKa = 3.66ASSDD97 pKa = 3.56SSFDD101 pKa = 3.76EE102 pKa = 4.25NSLADD107 pKa = 3.53SSSPQMQLRR116 pKa = 11.84AEE118 pKa = 4.17QMDD121 pKa = 4.09VASVPEE127 pKa = 4.36APPPQLCDD135 pKa = 3.44AEE137 pKa = 5.04EE138 pKa = 4.57EE139 pKa = 4.25DD140 pKa = 4.54SQPLRR145 pKa = 11.84SNATSRR151 pKa = 11.84KK152 pKa = 9.17SSISFIDD159 pKa = 3.22SSNPLLRR166 pKa = 11.84TPYY169 pKa = 11.21LMDD172 pKa = 5.09LCNDD176 pKa = 4.12DD177 pKa = 4.8YY178 pKa = 12.12NMTEE182 pKa = 3.93EE183 pKa = 5.31SSFEE187 pKa = 3.99LDD189 pKa = 3.28GVLDD193 pKa = 3.87YY194 pKa = 11.46LQIPP198 pKa = 4.21

Molecular weight: 21.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B4KLK2|B4KLK2_DROMO Uncharacterized protein isoform A OS=Drosophila mojavensis OX=7230 GN=Dmoj\GI19457 PE=3 SV=1
MM1 pKa = 7.73KK2 pKa = 10.3IFIVLALFIAAAAALPQFGFGGRR25 pKa = 11.84PGFGGPAFGGRR36 pKa = 11.84PGFGGPGFGGPGFGGPGFGGRR57 pKa = 11.84PGFGGGPGFGSRR69 pKa = 11.84PGFGGPGFGGPGFNRR84 pKa = 11.84GGGGGSASSSASASSSASGGGRR106 pKa = 11.84GGGASSASASASSSAGGGGFRR127 pKa = 11.84GG128 pKa = 3.63

Molecular weight: 11.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13363

4802

18165

11695963

23

15766

643.9

71.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.807 ± 0.022

1.825 ± 0.02

5.248 ± 0.014

6.455 ± 0.025

3.343 ± 0.014

5.724 ± 0.023

2.6 ± 0.01

4.986 ± 0.016

5.535 ± 0.022

9.201 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.011

4.93 ± 0.013

5.241 ± 0.02

5.59 ± 0.025

5.506 ± 0.018

8.219 ± 0.026

5.851 ± 0.018

5.714 ± 0.013

0.957 ± 0.006

2.938 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski