Parasphingorhabdus marina DSM 22363

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Parasphingorhabdus; Parasphingorhabdus marina

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6HFU3|A0A1N6HFU3_9SPHN Winged helix DNA-binding domain-containing protein OS=Parasphingorhabdus marina DSM 22363 OX=1123272 GN=SAMN02745824_3270 PE=4 SV=1
MM1 pKa = 7.49FVGTCDD7 pKa = 3.86SGWTRR12 pKa = 11.84SGAQSCVRR20 pKa = 11.84YY21 pKa = 9.04LAQLLLGLITLALFPAIAQAQTTTQYY47 pKa = 10.8TITDD51 pKa = 3.97PGVADD56 pKa = 6.11DD57 pKa = 3.96ITGSQICSSPLVQNFSVPDD76 pKa = 3.67NFTVSDD82 pKa = 3.57VDD84 pKa = 3.22IGVIATHH91 pKa = 5.68SWRR94 pKa = 11.84GDD96 pKa = 3.1MRR98 pKa = 11.84ITLEE102 pKa = 4.25SPLGTRR108 pKa = 11.84VRR110 pKa = 11.84VVNGSGGDD118 pKa = 3.28SGDD121 pKa = 3.75NFDD124 pKa = 5.18VRR126 pKa = 11.84LNDD129 pKa = 4.36GGTQLVNTDD138 pKa = 3.85PATGNHH144 pKa = 5.04NTGLPLYY151 pKa = 9.22EE152 pKa = 4.66NNFIPDD158 pKa = 3.46NALSAFNGEE167 pKa = 4.16NSTGTWRR174 pKa = 11.84LEE176 pKa = 3.61ICDD179 pKa = 4.27IFPSEE184 pKa = 4.8DD185 pKa = 3.19DD186 pKa = 4.2GNFLRR191 pKa = 11.84ADD193 pKa = 4.22LFLTDD198 pKa = 4.42TPPFADD204 pKa = 3.69LSVNKK209 pKa = 8.88TVNDD213 pKa = 3.59SSPNNGNNVTYY224 pKa = 10.06TITVTNASSSPSTATGIVVNDD245 pKa = 3.61QLPLGVTYY253 pKa = 10.97VSDD256 pKa = 3.84TGGGAYY262 pKa = 10.4NSGTGNWSVGTLAPGGSATLNIVANVTATSGATVTNAAEE301 pKa = 4.37VTVSSIFDD309 pKa = 3.92LDD311 pKa = 3.74STPGNGATGEE321 pKa = 4.3DD322 pKa = 4.73DD323 pKa = 4.15YY324 pKa = 11.88DD325 pKa = 3.94DD326 pKa = 5.43ASFTVAGTRR335 pKa = 11.84IAGTPPSLSTICSPANQILFDD356 pKa = 4.13WNGKK360 pKa = 8.76AWTTGSTNNNFTVAGIGVTNYY381 pKa = 10.99AITTDD386 pKa = 3.51VAFVTGSPAINSTNTGGQGATEE408 pKa = 3.76QSLFLNMNNNTRR420 pKa = 11.84SDD422 pKa = 3.26TSTTVLTLPTAVPGLQFTLFDD443 pKa = 3.54VDD445 pKa = 5.17FGAGQWADD453 pKa = 3.21KK454 pKa = 10.0VTVTGTFNGTTVIPTLTNGTANYY477 pKa = 10.19VIGNTAIGDD486 pKa = 3.94VGSDD490 pKa = 3.84GPDD493 pKa = 3.11PDD495 pKa = 4.17GNVVVTFLAPVDD507 pKa = 3.96TVTIVYY513 pKa = 9.97GNHH516 pKa = 4.11TTAPVNPGNQFMNIHH531 pKa = 6.85DD532 pKa = 4.44MNYY535 pKa = 9.29CQPDD539 pKa = 3.55TTFSVTKK546 pKa = 9.19ISSVISDD553 pKa = 3.74PVSIVDD559 pKa = 3.55GTSIPKK565 pKa = 9.9SIPGAIVQYY574 pKa = 10.52CILISNSGSATATDD588 pKa = 4.33IVATDD593 pKa = 3.81NLVGNYY599 pKa = 7.72TYY601 pKa = 10.29TAGSMQSGTNCNAAGTPEE619 pKa = 5.55DD620 pKa = 5.23DD621 pKa = 4.55DD622 pKa = 6.54ASDD625 pKa = 3.77GAEE628 pKa = 4.01SDD630 pKa = 3.92GVTASVIGTVLTANIASLGPSASTALTFRR659 pKa = 11.84VTVDD663 pKa = 2.78

Molecular weight: 68.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6CMI9|A0A1N6CMI9_9SPHN Iron complex outermembrane recepter protein OS=Parasphingorhabdus marina DSM 22363 OX=1123272 GN=SAMN02745824_0327 PE=3 SV=1
MM1 pKa = 7.3SFSLSAMLLLAAPMAAEE18 pKa = 3.83PLVPEE23 pKa = 4.52EE24 pKa = 4.45GARR27 pKa = 11.84SEE29 pKa = 4.97GIFVPPATWRR39 pKa = 11.84WAQVRR44 pKa = 11.84FRR46 pKa = 11.84KK47 pKa = 9.51RR48 pKa = 11.84VIIRR52 pKa = 11.84VPRR55 pKa = 11.84QRR57 pKa = 11.84PTRR60 pKa = 11.84PSTRR64 pKa = 11.84ASEE67 pKa = 4.42SRR69 pKa = 11.84TSAPLTYY76 pKa = 9.49RR77 pKa = 11.84EE78 pKa = 3.96KK79 pKa = 11.15RR80 pKa = 11.84MGKK83 pKa = 9.41CMPMNNILGVQMFGGRR99 pKa = 11.84NLDD102 pKa = 4.13LITRR106 pKa = 11.84DD107 pKa = 3.31RR108 pKa = 11.84KK109 pKa = 9.64RR110 pKa = 11.84VRR112 pKa = 11.84ARR114 pKa = 11.84LEE116 pKa = 4.1KK117 pKa = 10.25KK118 pKa = 9.16CQARR122 pKa = 11.84SFYY125 pKa = 10.97SGFYY129 pKa = 9.05MEE131 pKa = 5.09KK132 pKa = 10.3SEE134 pKa = 5.29DD135 pKa = 3.53GKK137 pKa = 10.78ICADD141 pKa = 3.75RR142 pKa = 11.84DD143 pKa = 3.64ILHH146 pKa = 6.64SRR148 pKa = 11.84TGSKK152 pKa = 10.8CEE154 pKa = 3.46IDD156 pKa = 3.36RR157 pKa = 11.84FRR159 pKa = 11.84EE160 pKa = 4.1LVPEE164 pKa = 4.27

Molecular weight: 18.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3398

0

3398

1084313

39

5177

319.1

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.133 ± 0.058

0.821 ± 0.014

6.362 ± 0.03

6.17 ± 0.048

3.903 ± 0.033

8.557 ± 0.068

1.932 ± 0.024

5.736 ± 0.038

3.798 ± 0.041

9.499 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.636 ± 0.024

3.204 ± 0.032

4.922 ± 0.041

3.421 ± 0.021

6.225 ± 0.044

5.984 ± 0.039

5.254 ± 0.052

6.724 ± 0.037

1.384 ± 0.019

2.336 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski