Fretibacterium sp. OH1220_COT-178

Taxonomy: cellular organisms; Bacteria; Synergistetes; Synergistia; Synergistales; Synergistaceae; Fretibacterium; unclassified Fretibacterium

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2505 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1Y5S5|A0A3P1Y5S5_9BACT V-type ATP synthase subunit D OS=Fretibacterium sp. OH1220_COT-178 OX=2491047 GN=atpD PE=3 SV=1
MM1 pKa = 7.02NAKK4 pKa = 9.56EE5 pKa = 3.94RR6 pKa = 11.84IAYY9 pKa = 9.65LRR11 pKa = 11.84GLLDD15 pKa = 3.77CMSLEE20 pKa = 4.1EE21 pKa = 4.7RR22 pKa = 11.84DD23 pKa = 3.82TKK25 pKa = 10.84IFAAVVEE32 pKa = 4.49ALDD35 pKa = 4.22ALACEE40 pKa = 4.84LEE42 pKa = 4.16GHH44 pKa = 6.21SDD46 pKa = 3.28LFEE49 pKa = 4.62LLRR52 pKa = 11.84EE53 pKa = 4.09NDD55 pKa = 3.18EE56 pKa = 4.27GLADD60 pKa = 4.08EE61 pKa = 5.77LYY63 pKa = 9.88EE64 pKa = 4.12LHH66 pKa = 7.73DD67 pKa = 4.22SVCEE71 pKa = 4.01LEE73 pKa = 4.77RR74 pKa = 11.84SIGLDD79 pKa = 3.28SEE81 pKa = 4.53QGCDD85 pKa = 3.31EE86 pKa = 4.71GDD88 pKa = 3.16VDD90 pKa = 4.58EE91 pKa = 4.46QAEE94 pKa = 4.68SYY96 pKa = 11.51VSVTCPSCAYY106 pKa = 10.0SFYY109 pKa = 10.76YY110 pKa = 9.96RR111 pKa = 11.84CEE113 pKa = 3.94EE114 pKa = 3.79DD115 pKa = 3.99RR116 pKa = 11.84EE117 pKa = 4.18EE118 pKa = 5.28GKK120 pKa = 10.05ILCPGCGEE128 pKa = 4.27EE129 pKa = 4.92IDD131 pKa = 3.89RR132 pKa = 11.84AVV134 pKa = 2.94

Molecular weight: 15.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1XYA7|A0A3P1XYA7_9BACT 50S ribosomal protein L32 OS=Fretibacterium sp. OH1220_COT-178 OX=2491047 GN=rpmF PE=3 SV=1
MM1 pKa = 7.76RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84GLFFLLALLPVATALICLGVGRR26 pKa = 11.84YY27 pKa = 8.45HH28 pKa = 7.42VSIRR32 pKa = 11.84EE33 pKa = 3.86TLEE36 pKa = 3.52ILFSALRR43 pKa = 11.84GEE45 pKa = 4.85SVASTAHH52 pKa = 6.13SVICKK57 pKa = 9.65VRR59 pKa = 11.84LPRR62 pKa = 11.84ILLSLFVGAGLSAAGASFQSLFSNPLATPDD92 pKa = 3.66TLGVATGASFGAVLALLFSQNLLAVQSSAMVMGLIALLATTFISRR137 pKa = 11.84VRR139 pKa = 11.84GKK141 pKa = 10.22SSVVMVVLAGMVVSALFQAMVSLAKK166 pKa = 10.49YY167 pKa = 9.25VADD170 pKa = 4.02PEE172 pKa = 4.43EE173 pKa = 4.29VLPAITYY180 pKa = 8.82WLMGSMSRR188 pKa = 11.84ASFHH192 pKa = 6.62GIAMGVPLIAAGVFVLFLLRR212 pKa = 11.84WRR214 pKa = 11.84LNILSLQEE222 pKa = 5.03DD223 pKa = 4.12EE224 pKa = 5.17AKK226 pKa = 10.83SLGIDD231 pKa = 3.51VKK233 pKa = 10.66KK234 pKa = 10.82LRR236 pKa = 11.84LAVMGAATLITASCVSLCGNVGWVGLLIPHH266 pKa = 7.88AARR269 pKa = 11.84MICGSDD275 pKa = 3.09NKK277 pKa = 10.56RR278 pKa = 11.84IVPVSLALGAAFMVLIDD295 pKa = 3.72TAARR299 pKa = 11.84SATAAEE305 pKa = 4.32IPVSILTAIIGAPFFIVLLRR325 pKa = 11.84RR326 pKa = 11.84TRR328 pKa = 11.84GARR331 pKa = 11.84LL332 pKa = 3.1

Molecular weight: 34.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2505

0

2505

840959

21

3295

335.7

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.189 ± 0.052

1.31 ± 0.018

5.23 ± 0.041

6.699 ± 0.068

3.991 ± 0.033

8.995 ± 0.044

1.71 ± 0.02

4.894 ± 0.042

3.741 ± 0.04

11.001 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.675 ± 0.024

2.628 ± 0.026

4.835 ± 0.04

2.333 ± 0.026

7.606 ± 0.068

5.864 ± 0.033

4.641 ± 0.039

7.903 ± 0.042

1.266 ± 0.02

2.488 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski