Corynebacterium phage LGCM-V4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W6JQX7|A0A1W6JQX7_9CAUD Uncharacterized protein OS=Corynebacterium phage LGCM-V4 OX=1972435 PE=4 SV=1
MM1 pKa = 8.17RR2 pKa = 11.84YY3 pKa = 9.22YY4 pKa = 10.46QDD6 pKa = 3.67PYY8 pKa = 11.4GDD10 pKa = 3.51IDD12 pKa = 3.77SVEE15 pKa = 4.05IRR17 pKa = 11.84FEE19 pKa = 4.02TCRR22 pKa = 11.84EE23 pKa = 3.92DD24 pKa = 3.69LARR27 pKa = 11.84AAQQCWRR34 pKa = 11.84KK35 pKa = 8.09LTSAEE40 pKa = 4.53LDD42 pKa = 3.57DD43 pKa = 6.13LYY45 pKa = 11.55DD46 pKa = 4.19EE47 pKa = 5.35IIRR50 pKa = 11.84VIAASEE56 pKa = 4.1WDD58 pKa = 4.07NMWLTVDD65 pKa = 5.0DD66 pKa = 5.69FITVASYY73 pKa = 11.18YY74 pKa = 9.82SHH76 pKa = 7.01EE77 pKa = 4.16

Molecular weight: 9.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W6JR03|A0A1W6JR03_9CAUD Uncharacterized protein OS=Corynebacterium phage LGCM-V4 OX=1972435 PE=4 SV=1
MM1 pKa = 7.55GKK3 pKa = 9.94YY4 pKa = 9.98DD5 pKa = 4.52KK6 pKa = 10.92QFQQLNRR13 pKa = 11.84NPKK16 pKa = 9.19IAQALNNRR24 pKa = 11.84AKK26 pKa = 10.53KK27 pKa = 9.76IRR29 pKa = 11.84AAAQRR34 pKa = 11.84ISDD37 pKa = 4.12AEE39 pKa = 4.29GGTANYY45 pKa = 9.96RR46 pKa = 11.84VVSGVRR52 pKa = 11.84PGGRR56 pKa = 11.84AYY58 pKa = 10.46AYY60 pKa = 9.85VVSDD64 pKa = 3.63NRR66 pKa = 11.84DD67 pKa = 3.37EE68 pKa = 4.55EE69 pKa = 4.69FGTQKK74 pKa = 9.56TKK76 pKa = 10.79RR77 pKa = 11.84IGALRR82 pKa = 11.84RR83 pKa = 11.84AARR86 pKa = 11.84GGG88 pKa = 3.39

Molecular weight: 9.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10886

37

1670

222.2

24.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.72 ± 0.808

0.717 ± 0.136

6.191 ± 0.342

6.357 ± 0.246

2.958 ± 0.193

7.514 ± 0.532

1.938 ± 0.143

6.008 ± 0.27

5.429 ± 0.257

8.46 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.26 ± 0.128

3.656 ± 0.249

4.483 ± 0.287

4.161 ± 0.252

5.732 ± 0.481

6.522 ± 0.249

5.916 ± 0.288

6.917 ± 0.265

1.902 ± 0.168

2.159 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski