Pseudomonas phage vB_PaeP_Tr60_Ab31

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6MWW3|W6MWW3_9CAUD Putative terminase small subunit OS=Pseudomonas phage vB_PaeP_Tr60_Ab31 OX=1449437 PE=4 SV=1
MM1 pKa = 6.84TTFTSIVTTNPDD13 pKa = 2.89FGGFEE18 pKa = 4.59FYY20 pKa = 11.21VEE22 pKa = 4.62AGQQFDD28 pKa = 3.43DD29 pKa = 3.78SAYY32 pKa = 8.49EE33 pKa = 3.7EE34 pKa = 4.93AYY36 pKa = 10.1GVSVPTAVVEE46 pKa = 4.2EE47 pKa = 4.46MNAKK51 pKa = 9.74AAQLKK56 pKa = 10.48DD57 pKa = 3.9GEE59 pKa = 4.43WLNVSHH65 pKa = 6.81EE66 pKa = 4.12AA67 pKa = 4.44

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6MVE9|W6MVE9_9CAUD TYR_PHOSPHATASE_2 domain-containing protein OS=Pseudomonas phage vB_PaeP_Tr60_Ab31 OX=1449437 PE=4 SV=1
MM1 pKa = 7.46EE2 pKa = 5.53PEE4 pKa = 4.97IIHH7 pKa = 5.77VPEE10 pKa = 4.03LAKK13 pKa = 11.03LLGRR17 pKa = 11.84TEE19 pKa = 3.92SSIRR23 pKa = 11.84SAIQSRR29 pKa = 11.84PDD31 pKa = 3.04WLPPYY36 pKa = 9.06FKK38 pKa = 10.63QGIRR42 pKa = 11.84VCWRR46 pKa = 11.84LEE48 pKa = 3.98SVRR51 pKa = 11.84KK52 pKa = 8.87FLRR55 pKa = 11.84DD56 pKa = 3.56YY57 pKa = 11.03EE58 pKa = 4.68AGEE61 pKa = 4.09HH62 pKa = 6.29KK63 pKa = 10.46APKK66 pKa = 9.13VGRR69 pKa = 11.84PRR71 pKa = 11.84RR72 pKa = 11.84EE73 pKa = 3.78PPRR76 pKa = 11.84LAKK79 pKa = 10.62VGG81 pKa = 3.51

Molecular weight: 9.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

14031

37

858

203.3

22.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.976 ± 0.597

1.254 ± 0.158

5.752 ± 0.243

7.348 ± 0.491

3.414 ± 0.237

7.662 ± 0.382

1.568 ± 0.164

5.124 ± 0.235

4.305 ± 0.307

7.989 ± 0.31

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.459 ± 0.178

3.507 ± 0.22

4.169 ± 0.18

4.604 ± 0.436

7.227 ± 0.356

6.521 ± 0.386

5.096 ± 0.406

6.201 ± 0.284

1.846 ± 0.174

2.951 ± 0.189
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski