Edwardsiella phage pEt-SU

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Petsuvirus; Edwardsiella virus pEtSU

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 284 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DWB3|A0A4D6DWB3_9CAUD Uncharacterized protein OS=Edwardsiella phage pEt-SU OX=2562142 GN=pETSU_070 PE=4 SV=1
MM1 pKa = 7.28FIEE4 pKa = 4.55YY5 pKa = 8.28VTLNLSFPTIQNTKK19 pKa = 7.86TLEE22 pKa = 4.06CLGQGLMVLLDD33 pKa = 3.32GHH35 pKa = 6.54FNNGLDD41 pKa = 3.9IDD43 pKa = 4.29DD44 pKa = 4.26TLLVHH49 pKa = 6.45NAKK52 pKa = 8.97LTMGDD57 pKa = 4.11CASEE61 pKa = 4.11LCMNNDD67 pKa = 3.19ADD69 pKa = 5.34FDD71 pKa = 4.57IIYY74 pKa = 10.56DD75 pKa = 3.75NASNALTLTLATYY88 pKa = 10.93CDD90 pKa = 4.58DD91 pKa = 3.76IKK93 pKa = 11.52NFALQLTSIADD104 pKa = 3.13IMMFEE109 pKa = 4.31YY110 pKa = 10.24PGMLRR115 pKa = 11.84FCVRR119 pKa = 11.84EE120 pKa = 3.94GVTQFGSGVLYY131 pKa = 10.42II132 pKa = 4.65

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DWY8|A0A4D6DWY8_9CAUD Uncharacterized protein OS=Edwardsiella phage pEt-SU OX=2562142 GN=pETSU_272 PE=4 SV=1
MM1 pKa = 7.25NRR3 pKa = 11.84TRR5 pKa = 11.84AYY7 pKa = 9.95RR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 5.88KK11 pKa = 10.79LQVKK15 pKa = 8.09KK16 pKa = 10.97AKK18 pKa = 9.97VSRR21 pKa = 11.84YY22 pKa = 6.64WNANTWEE29 pKa = 4.0YY30 pKa = 11.39GGYY33 pKa = 9.13QRR35 pKa = 11.84QHH37 pKa = 6.62DD38 pKa = 3.91KK39 pKa = 11.5AIIGRR44 pKa = 11.84VANTPKK50 pKa = 10.75ACACWMCANARR61 pKa = 11.84QVFGVPFADD70 pKa = 3.56VRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 9.21QLYY77 pKa = 10.25ADD79 pKa = 3.69QEE81 pKa = 4.27

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

284

0

284

86927

40

5119

306.1

34.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.645 ± 0.158

0.852 ± 0.055

6.45 ± 0.105

6.199 ± 0.219

4.389 ± 0.092

6.792 ± 0.182

1.877 ± 0.096

6.652 ± 0.093

6.502 ± 0.214

8.117 ± 0.184

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.84 ± 0.132

5.623 ± 0.119

4.051 ± 0.135

3.351 ± 0.072

4.357 ± 0.153

6.213 ± 0.104

6.649 ± 0.392

7.275 ± 0.249

1.24 ± 0.054

3.925 ± 0.1
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski