Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus; Lactococcus lactis; Lactococcus lactis subsp. lactis

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2225 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9CG76|Q9CG76_LACLA Uncharacterized protein OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=ymgB PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 10.19IEE4 pKa = 4.59LLEE7 pKa = 3.99EE8 pKa = 5.22DD9 pKa = 3.56IPEE12 pKa = 4.48FSNEE16 pKa = 3.8MMILTQVFATFKK28 pKa = 10.62CGEE31 pKa = 4.35DD32 pKa = 3.36LQMARR37 pKa = 11.84YY38 pKa = 8.8NFKK41 pKa = 10.18NQPEE45 pKa = 4.15IFQNYY50 pKa = 7.75PQVTQMLEE58 pKa = 4.15KK59 pKa = 10.42KK60 pKa = 10.23DD61 pKa = 4.03VLPLTLVDD69 pKa = 5.45GEE71 pKa = 4.19ITLIGDD77 pKa = 3.64YY78 pKa = 8.64PTVEE82 pKa = 5.03DD83 pKa = 3.68LTEE86 pKa = 3.78ITGLSFQNLGCEE98 pKa = 4.69EE99 pKa = 4.49DD100 pKa = 4.91CQDD103 pKa = 5.15CSCQQ107 pKa = 3.47

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9CJ98|Q9CJ98_LACLA Uncharacterized protein OS=Lactococcus lactis subsp. lactis (strain IL1403) OX=272623 GN=ybaD PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.06QPHH8 pKa = 5.63KK9 pKa = 9.98KK10 pKa = 9.63SRR12 pKa = 11.84KK13 pKa = 4.36TTHH16 pKa = 6.07GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.11NGRR28 pKa = 11.84RR29 pKa = 11.84VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.65GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2225

0

2225

656950

31

1983

295.3

33.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.396 ± 0.06

0.453 ± 0.014

5.315 ± 0.046

6.98 ± 0.065

4.784 ± 0.04

6.621 ± 0.05

1.772 ± 0.023

7.726 ± 0.049

7.368 ± 0.049

9.896 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.027

5.2 ± 0.051

3.234 ± 0.029

3.684 ± 0.031

3.583 ± 0.044

6.608 ± 0.076

5.732 ± 0.066

6.615 ± 0.042

0.999 ± 0.02

3.511 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski