Coprobacillus sp. CAG:235

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Erysipelotrichia; Erysipelotrichales; Coprobacillaceae; Coprobacillus; environmental samples

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|R5Q5N3|R5Q5N3_9FIRM Uncharacterized protein OS=Coprobacillus sp. CAG:235 OX=1262854 GN=BN550_01655 PE=4 SV=1
MM1 pKa = 7.09KK2 pKa = 10.23VVYY5 pKa = 10.35ASRR8 pKa = 11.84TGNVEE13 pKa = 4.33SIISKK18 pKa = 10.6LGIEE22 pKa = 4.64DD23 pKa = 3.67ALKK26 pKa = 11.07VEE28 pKa = 5.23DD29 pKa = 3.98GSEE32 pKa = 4.28TVEE35 pKa = 3.49EE36 pKa = 4.54DD37 pKa = 3.96YY38 pKa = 11.41ILFTYY43 pKa = 10.26TDD45 pKa = 3.48GFGDD49 pKa = 3.37VPYY52 pKa = 10.53EE53 pKa = 3.78VDD55 pKa = 4.89EE56 pKa = 4.49FLQSQSEE63 pKa = 4.24HH64 pKa = 6.18LKK66 pKa = 10.21GVVVSGDD73 pKa = 3.2TGYY76 pKa = 11.73GEE78 pKa = 5.26AYY80 pKa = 10.01CKK82 pKa = 10.66AGDD85 pKa = 4.37VIAEE89 pKa = 4.21QYY91 pKa = 10.64NVPCLYY97 pKa = 10.43KK98 pKa = 10.52VEE100 pKa = 4.55NDD102 pKa = 3.88GSDD105 pKa = 3.55EE106 pKa = 4.73DD107 pKa = 3.76IEE109 pKa = 4.76AIKK112 pKa = 10.61KK113 pKa = 9.76VIAA116 pKa = 4.19

Molecular weight:
12.72 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|R5Q5J6|R5Q5J6_9FIRM Uncharacterized protein OS=Coprobacillus sp. CAG:235 OX=1262854 GN=BN550_00005 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.34QPNKK9 pKa = 8.62RR10 pKa = 11.84KK11 pKa = 9.56RR12 pKa = 11.84AKK14 pKa = 8.76THH16 pKa = 5.23GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.34VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 8.97RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.13VLTAA44 pKa = 4.32

Molecular weight:
5.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2339

0

2339

677759

32

2948

289.8

33.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.4 ± 0.054

1.418 ± 0.017

6.013 ± 0.046

6.958 ± 0.055

4.42 ± 0.039

5.984 ± 0.056

2.173 ± 0.027

8.866 ± 0.056

8.538 ± 0.053

9.403 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.026

5.591 ± 0.046

2.698 ± 0.025

3.723 ± 0.033

3.143 ± 0.036

5.71 ± 0.044

4.969 ± 0.046

6.483 ± 0.042

0.632 ± 0.018

4.991 ± 0.052

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski