Streptomyces mangrovisoli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7461 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J4P6S9|A0A1J4P6S9_9ACTN Aminoglycoside phosphotransferase OS=Streptomyces mangrovisoli OX=1428628 GN=WN71_003825 PE=4 SV=1
MM1 pKa = 7.45SVPSARR7 pKa = 11.84PARR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84VSGAAPALALALVAAPAAVLAQAGPAGAAAATATATVQNGVAVYY56 pKa = 8.31TAAAGQTNKK65 pKa = 9.43VSATATDD72 pKa = 3.59LGGLQTIRR80 pKa = 11.84YY81 pKa = 8.77VIDD84 pKa = 3.33DD85 pKa = 4.42TVPITPGTGCTRR97 pKa = 11.84AGTDD101 pKa = 3.19ATQVVCDD108 pKa = 3.54VTTYY112 pKa = 11.26DD113 pKa = 3.86SQDD116 pKa = 3.13PYY118 pKa = 11.48ATLTVSLGDD127 pKa = 3.65RR128 pKa = 11.84NDD130 pKa = 3.4VVSYY134 pKa = 11.58ANTTGQDD141 pKa = 3.52YY142 pKa = 11.01YY143 pKa = 11.27FADD146 pKa = 4.05FDD148 pKa = 4.7LGSGNDD154 pKa = 3.54TFKK157 pKa = 10.91DD158 pKa = 3.36TGSVDD163 pKa = 3.08GNTVDD168 pKa = 3.71GGSGADD174 pKa = 3.64HH175 pKa = 6.35LTVGPVDD182 pKa = 3.59VALGGTGDD190 pKa = 3.85DD191 pKa = 3.94TIVAARR197 pKa = 11.84GTIAQGGAGRR207 pKa = 11.84DD208 pKa = 3.83TITLTGASAYY218 pKa = 10.54ADD220 pKa = 3.6GGADD224 pKa = 4.0ADD226 pKa = 4.5LLYY229 pKa = 11.1GGAGEE234 pKa = 4.23QSLSGGAGNDD244 pKa = 3.37TVRR247 pKa = 11.84AGTGNDD253 pKa = 2.63RR254 pKa = 11.84VYY256 pKa = 11.1GGTGNDD262 pKa = 3.02VLYY265 pKa = 10.96GNSGNDD271 pKa = 3.44TIYY274 pKa = 11.2GNSGNDD280 pKa = 3.09RR281 pKa = 11.84LYY283 pKa = 11.07GGPGTDD289 pKa = 3.65TLSGGPGSDD298 pKa = 4.59LVHH301 pKa = 6.67QSS303 pKa = 2.78

Molecular weight: 29.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J4P149|A0A1J4P149_9ACTN DNA ligase OS=Streptomyces mangrovisoli OX=1428628 GN=ligA PE=3 SV=1
MM1 pKa = 7.66KK2 pKa = 10.1VRR4 pKa = 11.84KK5 pKa = 9.01SLRR8 pKa = 11.84SLKK11 pKa = 10.27SKK13 pKa = 10.51PGAQVVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GVTFVVNKK30 pKa = 9.85KK31 pKa = 9.57NPRR34 pKa = 11.84FKK36 pKa = 10.85ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7461

0

7461

2489176

29

5523

333.6

35.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.943 ± 0.045

0.775 ± 0.007

6.11 ± 0.023

5.462 ± 0.027

2.722 ± 0.016

9.558 ± 0.027

2.365 ± 0.014

3.007 ± 0.021

2.052 ± 0.024

10.362 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.656 ± 0.011

1.703 ± 0.017

6.025 ± 0.027

2.714 ± 0.018

8.026 ± 0.038

5.063 ± 0.023

6.231 ± 0.029

8.57 ± 0.023

1.537 ± 0.011

2.118 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski