Punctularia strigosozonata (strain HHB-11173) (White-rot fungus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Corticiales; Punctulariaceae; Punctularia; Punctularia strigosozonata

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2093 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7RZT3|R7RZT3_PUNST Uncharacterized protein OS=Punctularia strigosozonata (strain HHB-11173) OX=741275 GN=PUNSTDRAFT_146953 PE=4 SV=1
MM1 pKa = 7.8LSPLIPLTLTVAIATLSHH19 pKa = 7.14ADD21 pKa = 3.4TVVIPTSSFDD31 pKa = 3.43SYY33 pKa = 12.14ADD35 pKa = 4.19LEE37 pKa = 4.85TYY39 pKa = 9.33WNYY42 pKa = 10.98LYY44 pKa = 10.25PWGSDD49 pKa = 3.3HH50 pKa = 6.98NGSARR55 pKa = 11.84MVGNSTDD62 pKa = 3.17HH63 pKa = 5.96SHH65 pKa = 6.95ISVANGVLTLTATPTSGQGTSSEE88 pKa = 4.52SPHH91 pKa = 5.72LTIDD95 pKa = 3.71YY96 pKa = 10.5FSGTIYY102 pKa = 10.86AKK104 pKa = 10.74DD105 pKa = 4.65DD106 pKa = 3.41ITVDD110 pKa = 3.23GTDD113 pKa = 3.1VVGYY117 pKa = 9.1VVEE120 pKa = 4.71GEE122 pKa = 4.9FIAPTAVGTWPAFWLTAVNSWPPEE146 pKa = 3.67ADD148 pKa = 2.63IGEE151 pKa = 4.35WKK153 pKa = 9.01GTQQTWFNTFNTSSEE168 pKa = 4.27VKK170 pKa = 9.03STIVAWPDD178 pKa = 3.04DD179 pKa = 4.25GEE181 pKa = 4.3FHH183 pKa = 6.73SVKK186 pKa = 10.51AVLSSIEE193 pKa = 4.15GDD195 pKa = 3.63SSDD198 pKa = 3.67VTIDD202 pKa = 3.63YY203 pKa = 11.21YY204 pKa = 11.46LDD206 pKa = 3.42NVLQATHH213 pKa = 6.19VGADD217 pKa = 3.57FLGAPLYY224 pKa = 10.65LIIDD228 pKa = 4.24LQMEE232 pKa = 5.0GSSGSPGPTGTTTYY246 pKa = 10.51QIRR249 pKa = 11.84NVQVTKK255 pKa = 10.12VTSS258 pKa = 3.3

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7S1Z8|R7S1Z8_PUNST Uncharacterized protein OS=Punctularia strigosozonata (strain HHB-11173) OX=741275 GN=PUNSTDRAFT_139108 PE=4 SV=1
MM1 pKa = 8.07RR2 pKa = 11.84SGACCAPLWPVQGGLRR18 pKa = 11.84WTACDD23 pKa = 3.06RR24 pKa = 11.84RR25 pKa = 11.84LRR27 pKa = 11.84EE28 pKa = 4.12LGRR31 pKa = 11.84LKK33 pKa = 10.57PRR35 pKa = 11.84IAIALHH41 pKa = 5.15STWSRR46 pKa = 11.84VYY48 pKa = 10.7GGLGVALSIKK58 pKa = 10.33DD59 pKa = 3.59ALVWTQTSKK68 pKa = 10.85HH69 pKa = 5.58YY70 pKa = 10.49NQSLDD75 pKa = 3.4FRR77 pKa = 11.84THH79 pKa = 7.04CSTTSHH85 pKa = 7.07LAFLPCIHH93 pKa = 6.74LHH95 pKa = 6.06FLPSRR100 pKa = 11.84PISHH104 pKa = 7.39PCVILSPTKK113 pKa = 9.38GTAFYY118 pKa = 10.21TRR120 pKa = 11.84VKK122 pKa = 9.93TSPAPLRR129 pKa = 11.84LRR131 pKa = 11.84LSRR134 pKa = 11.84GPSDD138 pKa = 4.36LRR140 pKa = 11.84IDD142 pKa = 3.92RR143 pKa = 11.84LRR145 pKa = 11.84ASPDD149 pKa = 3.22LGPGRR154 pKa = 11.84SAHH157 pKa = 7.6DD158 pKa = 3.64SDD160 pKa = 5.7LDD162 pKa = 3.95NNN164 pKa = 4.06

Molecular weight: 18.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2093

0

2093

867311

50

3615

414.4

45.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.641 ± 0.052

1.321 ± 0.022

5.892 ± 0.043

5.788 ± 0.063

3.493 ± 0.032

6.831 ± 0.056

2.634 ± 0.025

4.484 ± 0.04

4.028 ± 0.051

8.98 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.007 ± 0.022

3.148 ± 0.033

6.686 ± 0.069

3.58 ± 0.034

6.602 ± 0.054

8.217 ± 0.065

5.989 ± 0.045

6.537 ± 0.04

1.544 ± 0.023

2.598 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski