Phragmitibacter flavus

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Verrucomicrobiae; Verrucomicrobiales; Verrucomicrobiaceae; Phragmitibacter

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4729 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5R8K9V6|A0A5R8K9V6_9BACT Cytosolic protein OS=Phragmitibacter flavus OX=2576071 GN=FEM03_19405 PE=4 SV=1
MM1 pKa = 7.48LGAGLSLDD9 pKa = 4.44LKK11 pKa = 10.52SQTVSLGTAEE21 pKa = 4.05NFAVLGATTVTNTGASVLNGDD42 pKa = 4.7LGVSPGTALTGFFAVDD58 pKa = 3.82GGPGVVNGSIYY69 pKa = 10.55SAGAEE74 pKa = 3.67ALQAQEE80 pKa = 4.31DD81 pKa = 4.28AEE83 pKa = 4.5DD84 pKa = 4.09AFDD87 pKa = 5.95AIAAMAFTTDD97 pKa = 3.35LTGQNLGGLTLTPGVYY113 pKa = 10.35HH114 pKa = 7.2FDD116 pKa = 3.22TSAQLTGNLTLDD128 pKa = 3.55GVGEE132 pKa = 4.47YY133 pKa = 9.93IFQIGSTLTTASNASITGINGADD156 pKa = 2.91ASQFFFNVGSSATLGTGTQFAGTILALTSITLTTGADD193 pKa = 2.88IDD195 pKa = 3.97YY196 pKa = 10.96GRR198 pKa = 11.84AIALNGAVTMDD209 pKa = 3.61TNFINAVPEE218 pKa = 4.16PSSALLVGGALIYY231 pKa = 10.72FGMFRR236 pKa = 11.84SRR238 pKa = 11.84HH239 pKa = 4.76SMGSS243 pKa = 3.12

Molecular weight: 24.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5R8KEY2|A0A5R8KEY2_9BACT Uncharacterized protein OS=Phragmitibacter flavus OX=2576071 GN=FEM03_11190 PE=4 SV=1
MM1 pKa = 7.9PMPAIDD7 pKa = 3.6TTATAFFAGLVTSLHH22 pKa = 5.99CVGMCGPMACAWAGKK37 pKa = 10.13ASSQKK42 pKa = 8.0PTPFIRR48 pKa = 11.84DD49 pKa = 3.27TTLYY53 pKa = 10.04HH54 pKa = 7.01AARR57 pKa = 11.84LLSYY61 pKa = 9.17TVIGAIAGTIGIMPMQWFQHH81 pKa = 5.17GPALILPWLLVGLFIIIAFGLEE103 pKa = 3.34RR104 pKa = 11.84WLPKK108 pKa = 10.07PRR110 pKa = 11.84FLTMPMARR118 pKa = 11.84LKK120 pKa = 10.55LWALRR125 pKa = 11.84RR126 pKa = 11.84PPFHH130 pKa = 6.79RR131 pKa = 11.84AIIIGLATPLIPCGPLYY148 pKa = 11.18LMLGLAVANGSTIGGASFALAFGFGTLPLLWLMQTQLQWLNLKK191 pKa = 7.8LTPVRR196 pKa = 11.84LRR198 pKa = 11.84QVQRR202 pKa = 11.84SLALLAVFIMIWRR215 pKa = 11.84LRR217 pKa = 11.84GTFTGEE223 pKa = 3.88PDD225 pKa = 3.66TSCCHH230 pKa = 6.59PSLITGVPQQ239 pKa = 3.59

Molecular weight: 26.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4729

0

4729

1763432

34

10561

372.9

40.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.594 ± 0.033

0.89 ± 0.014

5.343 ± 0.028

6.039 ± 0.055

4.006 ± 0.02

8.131 ± 0.075

2.345 ± 0.025

5.092 ± 0.034

4.292 ± 0.046

10.318 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.349 ± 0.023

3.647 ± 0.053

5.173 ± 0.041

3.69 ± 0.025

5.939 ± 0.046

6.252 ± 0.04

5.989 ± 0.074

6.947 ± 0.04

1.613 ± 0.019

2.35 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski