Fusibacter sp. 3D3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XII. Incertae Sedis; Fusibacter; unclassified Fusibacter

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B6BE14|A0A1B6BE14_9FIRM Uncharacterized protein OS=Fusibacter sp. 3D3 OX=1048380 GN=F3D3_2584 PE=4 SV=1
MM1 pKa = 7.16SRR3 pKa = 11.84KK4 pKa = 9.47ILVLTFLFALLLITACSNSEE24 pKa = 3.97IEE26 pKa = 4.14TPIQVEE32 pKa = 4.57EE33 pKa = 4.28PSEE36 pKa = 4.07GTPATTASTDD46 pKa = 3.69EE47 pKa = 4.48IEE49 pKa = 4.67SSNTNEE55 pKa = 4.28APQDD59 pKa = 3.54SDD61 pKa = 3.69SATEE65 pKa = 3.99SSSAQQEE72 pKa = 4.15PYY74 pKa = 9.61IWEE77 pKa = 4.27DD78 pKa = 3.77FEE80 pKa = 6.03LKK82 pKa = 10.86NLDD85 pKa = 3.33GTTAQLYY92 pKa = 9.94DD93 pKa = 4.13FKK95 pKa = 11.68DD96 pKa = 3.75KK97 pKa = 10.94IVVINFWATWCHH109 pKa = 6.14FCDD112 pKa = 4.11QEE114 pKa = 4.22MPLIEE119 pKa = 5.76ALSQDD124 pKa = 3.88DD125 pKa = 4.59KK126 pKa = 11.46YY127 pKa = 11.39QVLAINVGEE136 pKa = 4.35DD137 pKa = 2.9TDD139 pKa = 4.58TIQKK143 pKa = 10.35YY144 pKa = 10.47LDD146 pKa = 3.63EE147 pKa = 6.01KK148 pKa = 11.02GLSLNVYY155 pKa = 10.11LDD157 pKa = 3.88EE158 pKa = 4.89DD159 pKa = 3.6QSLAGQFSVTGFPTTVFLGPNAEE182 pKa = 4.06YY183 pKa = 10.48LYY185 pKa = 10.03AYY187 pKa = 9.76PGMLDD192 pKa = 3.2QKK194 pKa = 9.6TLDD197 pKa = 4.54SILASIDD204 pKa = 3.34EE205 pKa = 4.29YY206 pKa = 11.28LAQSVLKK213 pKa = 10.77

Molecular weight: 23.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B6B7T8|A0A1B6B7T8_9FIRM Accessory gene regulator B OS=Fusibacter sp. 3D3 OX=1048380 GN=F3D3_0414 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 9.52QTFQPKK8 pKa = 8.5KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 7.92VEE15 pKa = 3.7HH16 pKa = 6.38GFRR19 pKa = 11.84KK20 pKa = 9.77RR21 pKa = 11.84MKK23 pKa = 10.02SSSGRR28 pKa = 11.84NILKK32 pKa = 9.76SRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.4GRR39 pKa = 11.84KK40 pKa = 8.87KK41 pKa = 10.58LSAA44 pKa = 3.95

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4633

0

4633

1463251

37

2939

315.8

35.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 0.04

0.984 ± 0.011

5.441 ± 0.027

7.121 ± 0.038

4.511 ± 0.028

6.398 ± 0.037

1.959 ± 0.017

8.809 ± 0.037

7.336 ± 0.037

9.681 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.978 ± 0.018

4.906 ± 0.029

3.124 ± 0.021

3.171 ± 0.021

3.587 ± 0.026

6.394 ± 0.031

5.447 ± 0.046

6.664 ± 0.027

0.762 ± 0.012

4.015 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski