Botryotinia fuckeliana totivirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Chrymotiviricetes; Ghabrivirales; Totiviridae; Totivirus; unclassified Totivirus

Average proteome isoelectric point is 7.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4FRF6|A4FRF6_9VIRU RNA-directed RNA polymerase OS=Botryotinia fuckeliana totivirus 1 OX=425009 GN=RdRp PE=3 SV=1
MM1 pKa = 7.71SSQLNPTFLSGVLANARR18 pKa = 11.84GGRR21 pKa = 11.84ITTKK25 pKa = 9.73DD26 pKa = 2.57TYY28 pKa = 10.88RR29 pKa = 11.84RR30 pKa = 11.84YY31 pKa = 8.79KK32 pKa = 10.18TIVRR36 pKa = 11.84TSATIAGVLDD46 pKa = 3.57TRR48 pKa = 11.84LTGIFYY54 pKa = 10.12EE55 pKa = 4.25IGRR58 pKa = 11.84AVDD61 pKa = 3.49TKK63 pKa = 11.14GKK65 pKa = 9.74LLKK68 pKa = 10.08SHH70 pKa = 6.98KK71 pKa = 10.23EE72 pKa = 3.92DD73 pKa = 3.08ITLVEE78 pKa = 4.28ASYY81 pKa = 8.46PTNSVLAEE89 pKa = 4.3DD90 pKa = 5.33FIGMAKK96 pKa = 10.32KK97 pKa = 8.94YY98 pKa = 8.7TNFSASFEE106 pKa = 4.23YY107 pKa = 10.72SSLAGVVEE115 pKa = 4.18RR116 pKa = 11.84LGKK119 pKa = 10.5GLAACSLFDD128 pKa = 4.53SVTSTDD134 pKa = 3.34LRR136 pKa = 11.84GGAVLAVNALGTYY149 pKa = 9.7DD150 pKa = 4.08GPVNSLTDD158 pKa = 3.24AVYY161 pKa = 10.19IPRR164 pKa = 11.84LVNTTLTGDD173 pKa = 3.89VFAVLCNAVAGEE185 pKa = 4.54GSQVVSDD192 pKa = 4.33VVEE195 pKa = 4.18LDD197 pKa = 3.1ANTRR201 pKa = 11.84QPIIALVDD209 pKa = 3.87SVGLPGAIVDD219 pKa = 3.98ALRR222 pKa = 11.84VLGSNMIASDD232 pKa = 3.62QGPLFSLALARR243 pKa = 11.84GLHH246 pKa = 6.33RR247 pKa = 11.84GLSLVGHH254 pKa = 5.99TDD256 pKa = 2.87EE257 pKa = 6.06GSITRR262 pKa = 11.84DD263 pKa = 3.25LLRR266 pKa = 11.84CGGFAPPFGGIHH278 pKa = 6.3YY279 pKa = 10.33GLEE282 pKa = 4.37PYY284 pKa = 10.51VGLPALQTNLDD295 pKa = 4.12KK296 pKa = 11.28GVSAYY301 pKa = 10.79VDD303 pKa = 4.18AIVIATGAAVAHH315 pKa = 7.07ADD317 pKa = 3.17PGTRR321 pKa = 11.84FDD323 pKa = 3.92GHH325 pKa = 6.75WFPTFISGTASGEE338 pKa = 3.81PLLRR342 pKa = 11.84PGEE345 pKa = 4.33HH346 pKa = 5.24TTGTTAMADD355 pKa = 4.17RR356 pKa = 11.84IRR358 pKa = 11.84ARR360 pKa = 11.84LLADD364 pKa = 3.62QSPFWEE370 pKa = 5.9GYY372 pKa = 7.77TRR374 pKa = 11.84ALSIIFGAEE383 pKa = 3.63GDD385 pKa = 3.69TSLSVRR391 pKa = 11.84FACAASTALDD401 pKa = 3.28VDD403 pKa = 3.75NRR405 pKa = 11.84HH406 pKa = 6.28LKK408 pKa = 9.19HH409 pKa = 6.66ASVSPWFWVEE419 pKa = 3.74PTSILPPNLLGSEE432 pKa = 4.39AEE434 pKa = 4.41LEE436 pKa = 4.67GCASLAHH443 pKa = 7.03KK444 pKa = 9.32DD445 pKa = 3.71TVVSKK450 pKa = 9.35PAWDD454 pKa = 3.89TITRR458 pKa = 11.84VGSSDD463 pKa = 3.41VSFSAYY469 pKa = 9.45RR470 pKa = 11.84ARR472 pKa = 11.84FKK474 pKa = 10.54FARR477 pKa = 11.84QTWFFNHH484 pKa = 6.57WLNHH488 pKa = 4.87PMNGLGAMKK497 pKa = 10.08VRR499 pKa = 11.84QLDD502 pKa = 3.65PNGIIHH508 pKa = 7.11PGPCTAHH515 pKa = 5.62PQVRR519 pKa = 11.84DD520 pKa = 3.33RR521 pKa = 11.84VEE523 pKa = 4.5ADD525 pKa = 4.14LPFTDD530 pKa = 4.37YY531 pKa = 11.4LWIRR535 pKa = 11.84GQSPFPAPGEE545 pKa = 4.17LINLGLGVGFLVRR558 pKa = 11.84HH559 pKa = 5.11MTLDD563 pKa = 3.34AEE565 pKa = 4.84GIPNEE570 pKa = 4.02EE571 pKa = 4.32HH572 pKa = 6.77LPTSRR577 pKa = 11.84EE578 pKa = 3.76IQDD581 pKa = 3.76CVVTIDD587 pKa = 3.11VGRR590 pKa = 11.84PIGLASGKK598 pKa = 8.58TNAADD603 pKa = 3.78SQAKK607 pKa = 9.35RR608 pKa = 11.84ARR610 pKa = 11.84TRR612 pKa = 11.84ANNEE616 pKa = 3.27LAAARR621 pKa = 11.84TRR623 pKa = 11.84ARR625 pKa = 11.84VFGTPDD631 pKa = 3.13VGEE634 pKa = 4.44MPTLTTAPGYY644 pKa = 7.16ATRR647 pKa = 11.84WSSEE651 pKa = 3.57QGIDD655 pKa = 3.51STSGGVVSRR664 pKa = 11.84GRR666 pKa = 11.84HH667 pKa = 4.21DD668 pKa = 3.44HH669 pKa = 6.6AAPGHH674 pKa = 6.12ASGGDD679 pKa = 3.36RR680 pKa = 11.84EE681 pKa = 4.53PTGKK685 pKa = 10.2QNIAVPQHH693 pKa = 5.17QPLRR697 pKa = 11.84YY698 pKa = 8.31PALPRR703 pKa = 11.84QGAGLGGGANPIPPPPAPPAGGPDD727 pKa = 4.87DD728 pKa = 5.66GPQSDD733 pKa = 5.39DD734 pKa = 4.61DD735 pKa = 4.28NTPSAPTAPLGLSTTPPRR753 pKa = 11.84SPAAPGNGPDD763 pKa = 3.96TII765 pKa = 4.52

Molecular weight: 80.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4FRF6|A4FRF6_9VIRU RNA-directed RNA polymerase OS=Botryotinia fuckeliana totivirus 1 OX=425009 GN=RdRp PE=3 SV=1
MM1 pKa = 7.3SAADD5 pKa = 3.72RR6 pKa = 11.84ASAYY10 pKa = 10.17GRR12 pKa = 11.84LGSYY16 pKa = 10.28LKK18 pKa = 10.57EE19 pKa = 4.34LLDD22 pKa = 3.97DD23 pKa = 4.22HH24 pKa = 7.14PFVQGLFSTTNFTQSLIRR42 pKa = 11.84LQGSSFTMRR51 pKa = 11.84SAHH54 pKa = 6.73PLLPHH59 pKa = 6.88AASLLLLDD67 pKa = 6.0FPMQTDD73 pKa = 3.76TSVSDD78 pKa = 4.13VISLARR84 pKa = 11.84NAYY87 pKa = 9.56DD88 pKa = 4.94LSDD91 pKa = 3.88LGEE94 pKa = 4.51DD95 pKa = 3.41VLINHH100 pKa = 6.92TLLPPGSPILSWRR113 pKa = 11.84RR114 pKa = 11.84RR115 pKa = 11.84GYY117 pKa = 9.79HH118 pKa = 6.15RR119 pKa = 11.84KK120 pKa = 9.72LGNAIITNKK129 pKa = 8.69EE130 pKa = 3.79LRR132 pKa = 11.84DD133 pKa = 3.5SLFPKK138 pKa = 9.9KK139 pKa = 9.99KK140 pKa = 9.98HH141 pKa = 5.31NAAGVKK147 pKa = 10.13VNLTIGRR154 pKa = 11.84LAVAWARR161 pKa = 11.84VFGPGSLGRR170 pKa = 11.84HH171 pKa = 5.57LAAIAGRR178 pKa = 11.84VTNDD182 pKa = 3.25QACSALLYY190 pKa = 10.63CLTLRR195 pKa = 11.84EE196 pKa = 4.66HH197 pKa = 6.66IGSFGISIAQAAVTQPANAKK217 pKa = 9.8GLSNALKK224 pKa = 10.61ALGANSSLPGSLLVEE239 pKa = 4.38AATLQGRR246 pKa = 11.84FTNDD250 pKa = 1.91VDD252 pKa = 3.64MTNEE256 pKa = 3.4IRR258 pKa = 11.84SRR260 pKa = 11.84TIQSLVDD267 pKa = 3.43EE268 pKa = 4.43QVIDD272 pKa = 3.71RR273 pKa = 11.84PEE275 pKa = 3.76EE276 pKa = 3.76LRR278 pKa = 11.84PHH280 pKa = 6.48IKK282 pKa = 10.54ALLEE286 pKa = 3.76MSLPGDD292 pKa = 3.78CALPDD297 pKa = 3.53MDD299 pKa = 4.75EE300 pKa = 4.09WWSSRR305 pKa = 11.84WLWCVNGSEE314 pKa = 4.45TKK316 pKa = 10.65KK317 pKa = 10.67SDD319 pKa = 3.16EE320 pKa = 4.05ALGLRR325 pKa = 11.84GGSGRR330 pKa = 11.84RR331 pKa = 11.84YY332 pKa = 9.24RR333 pKa = 11.84RR334 pKa = 11.84MAAEE338 pKa = 4.31EE339 pKa = 4.16VHH341 pKa = 6.71SNPVPDD347 pKa = 3.61WDD349 pKa = 3.84GTTSVSASIKK359 pKa = 10.73LEE361 pKa = 4.13AGKK364 pKa = 10.4DD365 pKa = 3.39RR366 pKa = 11.84AIFACDD372 pKa = 3.05TRR374 pKa = 11.84SYY376 pKa = 11.08FAFSWILNDD385 pKa = 4.35VQKK388 pKa = 10.49KK389 pKa = 6.96WKK391 pKa = 10.19GEE393 pKa = 4.16RR394 pKa = 11.84IPLDD398 pKa = 3.79PGKK401 pKa = 10.75GGLYY405 pKa = 10.22GISRR409 pKa = 11.84RR410 pKa = 11.84IRR412 pKa = 11.84NSQRR416 pKa = 11.84GGGVNLMLDD425 pKa = 3.48YY426 pKa = 11.6DD427 pKa = 4.41NFNSHH432 pKa = 6.79HH433 pKa = 6.59SNSVQSMIFEE443 pKa = 4.38VLCDD447 pKa = 4.44KK448 pKa = 11.25YY449 pKa = 11.22NAPQWYY455 pKa = 9.66KK456 pKa = 10.27EE457 pKa = 3.79VLMEE461 pKa = 4.46SFNRR465 pKa = 11.84MYY467 pKa = 10.39IFKK470 pKa = 10.56GGVRR474 pKa = 11.84HH475 pKa = 6.73RR476 pKa = 11.84MLGTLASGHH485 pKa = 6.67RR486 pKa = 11.84GTSFINSLLNAAYY499 pKa = 9.36IRR501 pKa = 11.84CALGAAKK508 pKa = 9.93FDD510 pKa = 3.99TMLSLHH516 pKa = 6.87AGDD519 pKa = 4.95DD520 pKa = 3.76VYY522 pKa = 11.3IRR524 pKa = 11.84ANTLSEE530 pKa = 4.05AADD533 pKa = 3.34ILKK536 pKa = 9.36KK537 pKa = 10.73CKK539 pKa = 9.85MFGCRR544 pKa = 11.84MNPTKK549 pKa = 10.58QSIGFRR555 pKa = 11.84NAEE558 pKa = 4.11FLRR561 pKa = 11.84LGINSRR567 pKa = 11.84YY568 pKa = 9.44AVGYY572 pKa = 6.92VARR575 pKa = 11.84TISTLVAGNWSNLDD589 pKa = 3.19PMEE592 pKa = 4.56PLEE595 pKa = 4.49SLTSVISSVRR605 pKa = 11.84SVINRR610 pKa = 11.84GAPTIIADD618 pKa = 4.29VIAYY622 pKa = 8.64AHH624 pKa = 6.09STLHH628 pKa = 6.66PYY630 pKa = 8.78PLKK633 pKa = 10.35ILKK636 pKa = 9.48PLLRR640 pKa = 11.84GEE642 pKa = 4.1AAIAGAPIFNLHH654 pKa = 5.14GKK656 pKa = 7.79MRR658 pKa = 11.84TYY660 pKa = 10.44EE661 pKa = 4.11AVVSKK666 pKa = 10.25PDD668 pKa = 3.4NSGNPPPPEE677 pKa = 4.23WGSHH681 pKa = 4.08ATLSYY686 pKa = 10.97LEE688 pKa = 4.21YY689 pKa = 10.37HH690 pKa = 6.2VAPIEE695 pKa = 3.97ASAISRR701 pKa = 11.84ARR703 pKa = 11.84VDD705 pKa = 3.55LTSIMQEE712 pKa = 3.32SSYY715 pKa = 11.19AKK717 pKa = 9.55QVRR720 pKa = 11.84SDD722 pKa = 3.86LDD724 pKa = 3.53DD725 pKa = 4.13ASRR728 pKa = 11.84RR729 pKa = 11.84TTVKK733 pKa = 10.7LIPRR737 pKa = 11.84TPEE740 pKa = 3.4EE741 pKa = 3.58ARR743 pKa = 11.84GYY745 pKa = 10.26IDD747 pKa = 5.58ASTLSTRR754 pKa = 11.84DD755 pKa = 3.14AKK757 pKa = 10.77RR758 pKa = 11.84GILEE762 pKa = 4.24SYY764 pKa = 10.04PLIRR768 pKa = 11.84LLEE771 pKa = 4.17NRR773 pKa = 11.84LSDD776 pKa = 3.44EE777 pKa = 4.49DD778 pKa = 3.97LRR780 pKa = 11.84EE781 pKa = 3.96LALLLGVSCSTKK793 pKa = 9.4EE794 pKa = 4.34ARR796 pKa = 11.84VCCFGSEE803 pKa = 4.27SLTHH807 pKa = 6.59NIIGYY812 pKa = 9.47LPYY815 pKa = 10.56SDD817 pKa = 4.55AASMSKK823 pKa = 7.23TTNSRR828 pKa = 11.84NIYY831 pKa = 5.19TTYY834 pKa = 10.46HH835 pKa = 4.55VRR837 pKa = 11.84AA838 pKa = 4.25

Molecular weight: 92.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1603

765

838

801.5

86.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.168 ± 0.527

1.185 ± 0.175

5.677 ± 0.303

4.117 ± 0.297

3.057 ± 0.222

8.734 ± 1.204

2.62 ± 0.004

5.115 ± 0.436

3.306 ± 0.619

10.168 ± 0.746

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.415

4.055 ± 0.426

6.051 ± 1.079

2.183 ± 0.11

6.925 ± 0.422

8.609 ± 0.922

6.488 ± 0.88

5.802 ± 0.647

1.31 ± 0.087

2.745 ± 0.424
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski