Vagococcus fluvialis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales;

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2526 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A429ZY50|A0A429ZY50_9ENTE Methylase_S domain-containing protein OS=Vagococcus fluvialis OX=2738 GN=CBF32_12480 PE=4 SV=1
MM1 pKa = 7.78SDD3 pKa = 3.35THH5 pKa = 6.98NHH7 pKa = 5.85DD8 pKa = 3.63HH9 pKa = 7.15DD10 pKa = 5.89HH11 pKa = 6.31IHH13 pKa = 7.27DD14 pKa = 4.57HH15 pKa = 7.19DD16 pKa = 6.32ADD18 pKa = 4.17DD19 pKa = 6.46LITLIDD25 pKa = 3.96DD26 pKa = 4.09EE27 pKa = 4.86GNEE30 pKa = 3.99VLFRR34 pKa = 11.84IHH36 pKa = 6.3LTIDD40 pKa = 3.2GQEE43 pKa = 4.29EE44 pKa = 4.31YY45 pKa = 11.2GKK47 pKa = 10.45DD48 pKa = 3.53YY49 pKa = 11.46VLLYY53 pKa = 10.45DD54 pKa = 4.1ASSPEE59 pKa = 4.05GEE61 pKa = 4.26EE62 pKa = 4.33VEE64 pKa = 4.32LLAYY68 pKa = 10.02AYY70 pKa = 7.29EE71 pKa = 4.32QKK73 pKa = 10.78DD74 pKa = 3.67GEE76 pKa = 4.37AEE78 pKa = 4.02GRR80 pKa = 11.84LTEE83 pKa = 4.48IEE85 pKa = 4.88TEE87 pKa = 4.38AEE89 pKa = 3.12WDD91 pKa = 3.58MIEE94 pKa = 4.12EE95 pKa = 4.14VFNAFEE101 pKa = 4.41AEE103 pKa = 4.14EE104 pKa = 4.11EE105 pKa = 4.24

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369AQN8|A0A369AQN8_9ENTE TIGR01212 family radical SAM protein OS=Vagococcus fluvialis OX=2738 GN=CBF32_11905 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.15GRR39 pKa = 11.84KK40 pKa = 8.82ALAAA44 pKa = 4.25

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2526

0

2526

783260

37

2113

310.1

34.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.152 ± 0.051

0.597 ± 0.011

5.375 ± 0.035

7.956 ± 0.054

4.707 ± 0.038

6.392 ± 0.047

1.723 ± 0.019

8.207 ± 0.045

7.681 ± 0.043

9.882 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.797 ± 0.021

5.093 ± 0.035

3.222 ± 0.025

3.45 ± 0.028

3.305 ± 0.034

6.317 ± 0.035

5.778 ± 0.038

6.902 ± 0.04

0.829 ± 0.015

3.636 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski