Flavobacterium commune

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3082 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1D9P7R4|A0A1D9P7R4_9FLAO 50S ribosomal protein L15 OS=Flavobacterium commune OX=1306519 GN=rplO PE=3 SV=1
MM1 pKa = 7.61KK2 pKa = 10.16KK3 pKa = 8.74IQHH6 pKa = 7.1IITILCLLFLLPCCEE21 pKa = 4.1NDD23 pKa = 3.87GGDD26 pKa = 4.59SKK28 pKa = 11.33ISTQKK33 pKa = 10.67GALPNIQKK41 pKa = 10.58LEE43 pKa = 4.06NSDD46 pKa = 3.84SFIDD50 pKa = 4.8LVAVQNNQEE59 pKa = 4.02INLGFSVDD67 pKa = 3.78LAIGKK72 pKa = 9.42ISSMDD77 pKa = 3.47IIGLYY82 pKa = 9.73IKK84 pKa = 10.66SDD86 pKa = 3.28GTIIKK91 pKa = 8.53ATLASNITSFPVSFNLNRR109 pKa = 11.84NDD111 pKa = 4.7LYY113 pKa = 11.46DD114 pKa = 3.66AFEE117 pKa = 4.56NLNSSEE123 pKa = 4.42DD124 pKa = 3.77FATGDD129 pKa = 3.44QLIISADD136 pKa = 3.1ITLKK140 pKa = 10.95DD141 pKa = 3.53GTVLKK146 pKa = 10.71IMNDD150 pKa = 3.3DD151 pKa = 3.52GTNNFSSNIATSNLYY166 pKa = 10.36KK167 pKa = 10.55VFQTYY172 pKa = 9.8NVSCPSYY179 pKa = 10.82LAGTYY184 pKa = 10.14NYY186 pKa = 8.05STTNIGDD193 pKa = 3.56GSYY196 pKa = 8.64FTSDD200 pKa = 3.05VFTGTVTFEE209 pKa = 4.08DD210 pKa = 3.4QGGGVYY216 pKa = 10.15IISDD220 pKa = 3.92GTFGGYY226 pKa = 6.78TALYY230 pKa = 9.73DD231 pKa = 3.48EE232 pKa = 4.57TAIGVEE238 pKa = 4.84LKK240 pKa = 10.51DD241 pKa = 3.11ICNQISFQGSNQYY254 pKa = 11.38GDD256 pKa = 3.73TFTMSNLVVNGNKK269 pKa = 8.4LTFHH273 pKa = 7.09WEE275 pKa = 3.85TSFGEE280 pKa = 4.38FGDD283 pKa = 4.0TTLTRR288 pKa = 11.84TDD290 pKa = 3.08NTNWPNLTLL299 pKa = 4.07

Molecular weight:
32.66 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1D9P6S7|A0A1D9P6S7_9FLAO Transcriptional regulator OS=Flavobacterium commune OX=1306519 GN=BIW12_01915 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.91HH17 pKa = 4.39GFMDD21 pKa = 4.5RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.19GRR40 pKa = 11.84HH41 pKa = 5.41KK42 pKa = 10.14LTVSSEE48 pKa = 3.92PRR50 pKa = 11.84HH51 pKa = 5.77KK52 pKa = 10.61KK53 pKa = 9.84

Molecular weight:
6.3 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3082

0

3082

1113438

42

3917

361.3

40.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.704 ± 0.05

0.781 ± 0.017

5.303 ± 0.029

6.345 ± 0.045

5.217 ± 0.034

6.379 ± 0.041

1.648 ± 0.021

8.027 ± 0.046

8.043 ± 0.052

9.003 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.162 ± 0.024

6.498 ± 0.045

3.43 ± 0.023

3.466 ± 0.023

3.143 ± 0.027

6.502 ± 0.037

5.912 ± 0.079

6.211 ± 0.043

1.137 ± 0.021

4.09 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski