Mint virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Closterovirus

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q5G7G1|Q5G7G1_9CLOS Coat protein OS=Mint virus 1 OX=300740 PE=4 SV=1
MM1 pKa = 7.7SEE3 pKa = 3.87VSFSIRR9 pKa = 11.84PIADD13 pKa = 3.2GDD15 pKa = 4.06TNTIVIIRR23 pKa = 11.84DD24 pKa = 3.48KK25 pKa = 10.86FGHH28 pKa = 5.79SVLNFKK34 pKa = 10.78SPAGDD39 pKa = 3.6DD40 pKa = 3.47SLALVEE46 pKa = 4.35EE47 pKa = 4.48SCIISRR53 pKa = 11.84SDD55 pKa = 3.5FEE57 pKa = 5.46SVASCDD63 pKa = 4.13DD64 pKa = 3.77SFFLAGLNSLEE75 pKa = 4.24DD76 pKa = 3.57ALNNFVLHH84 pKa = 6.19SPRR87 pKa = 11.84SLTATSEE94 pKa = 4.39SLSSLEE100 pKa = 4.32YY101 pKa = 9.97IDD103 pKa = 6.04FVLDD107 pKa = 4.36PIMLGIIFRR116 pKa = 11.84FNLKK120 pKa = 10.07RR121 pKa = 11.84RR122 pKa = 11.84INNDD126 pKa = 2.89SVSLFRR132 pKa = 11.84DD133 pKa = 3.31SLISLNGGWYY143 pKa = 10.5NGTLYY148 pKa = 11.17DD149 pKa = 5.79DD150 pKa = 3.81MFHH153 pKa = 6.43YY154 pKa = 10.78LSNFFKK160 pKa = 10.53QKK162 pKa = 10.34KK163 pKa = 9.35GFTIHH168 pKa = 6.45SFEE171 pKa = 5.49LIKK174 pKa = 10.82QNEE177 pKa = 4.46VVFPSRR183 pKa = 11.84VLRR186 pKa = 11.84VSGDD190 pKa = 3.42RR191 pKa = 11.84GHH193 pKa = 6.43QPIDD197 pKa = 3.12

Molecular weight:
22.17 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q5G7G6|Q5G7G6_9CLOS Polymerase (Fragment) OS=Mint virus 1 OX=300740 PE=4 SV=1
MM1 pKa = 8.04DD2 pKa = 4.1CTLRR6 pKa = 11.84AYY8 pKa = 10.31FYY10 pKa = 11.39LLLGWIIVCFSFTLGFVVYY29 pKa = 10.19KK30 pKa = 10.19LVRR33 pKa = 11.84TCSNVYY39 pKa = 10.71GDD41 pKa = 5.02IIDD44 pKa = 3.86TSVVGTSRR52 pKa = 11.84RR53 pKa = 11.84IDD55 pKa = 3.11IEE57 pKa = 3.88NRR59 pKa = 11.84GNSSARR65 pKa = 11.84VV66 pKa = 3.35

Molecular weight:
7.49 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4981

66

2511

553.4

62.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.682 ± 0.555

2.53 ± 0.181

5.983 ± 0.353

5.722 ± 0.259

6.204 ± 0.423

5.14 ± 0.235

2.148 ± 0.231

5.441 ± 0.313

5.682 ± 0.422

8.894 ± 0.649

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.927 ± 0.145

4.357 ± 0.325

3.714 ± 0.293

1.927 ± 0.173

6.545 ± 0.45

10.359 ± 0.76

5.32 ± 0.314

8.532 ± 0.657

0.482 ± 0.185

3.413 ± 0.199

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski