Phycisphaerae bacterium RAS2

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; unclassified Phycisphaerae

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3554 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518LJG2|A0A518LJG2_9BACT UDP-2 3-diacetamido-2 3-dideoxy-D-glucuronate 2-epimerase OS=Phycisphaerae bacterium RAS2 OX=2528035 GN=wbpI_1 PE=3 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84KK3 pKa = 9.76ALTWVIAGLLGVAVSASQAQTLYY26 pKa = 8.27TTGFDD31 pKa = 3.31GSPFVNGNLSGQDD44 pKa = 2.83GWVTTDD50 pKa = 3.11SPEE53 pKa = 4.13TPFLGTVQGSFSFSPSRR70 pKa = 11.84SVRR73 pKa = 11.84INASQTIDD81 pKa = 3.89SNWWWTPLNHH91 pKa = 6.9SIHH94 pKa = 6.54GSATVIQIEE103 pKa = 4.23CMIYY107 pKa = 9.73VDD109 pKa = 4.3RR110 pKa = 11.84ASGSASTLWGVDD122 pKa = 2.97IYY124 pKa = 11.42DD125 pKa = 3.74GSLPLTRR132 pKa = 11.84RR133 pKa = 11.84VATIAIDD140 pKa = 3.7TSNHH144 pKa = 5.22LLVWDD149 pKa = 4.07SVAFVDD155 pKa = 4.01TGVIVPPNTWGLFRR169 pKa = 11.84MNLNYY174 pKa = 10.48AAGEE178 pKa = 4.3RR179 pKa = 11.84NTSVYY184 pKa = 10.58FNGARR189 pKa = 11.84VAYY192 pKa = 9.82RR193 pKa = 11.84RR194 pKa = 11.84AFGLGANDD202 pKa = 3.31ILADD206 pKa = 3.36VDD208 pKa = 5.05FYY210 pKa = 11.6HH211 pKa = 7.54VDD213 pKa = 2.91AGGNDD218 pKa = 3.09SAYY221 pKa = 10.31FDD223 pKa = 3.97NFVVRR228 pKa = 11.84AMADD232 pKa = 3.2GDD234 pKa = 4.1GDD236 pKa = 4.38GVPDD240 pKa = 4.69SQDD243 pKa = 3.02SCLNTAAGDD252 pKa = 4.19PINATGCSTLDD263 pKa = 3.4NDD265 pKa = 5.34GDD267 pKa = 4.38GVLNDD272 pKa = 5.12ADD274 pKa = 4.26NCPDD278 pKa = 3.68TPTCAIVNGAGCPFDD293 pKa = 4.82SDD295 pKa = 3.73GDD297 pKa = 4.23SVMNGCDD304 pKa = 3.01NCDD307 pKa = 3.36NVPNGPGEE315 pKa = 4.63DD316 pKa = 3.67NQADD320 pKa = 3.83ADD322 pKa = 3.92SDD324 pKa = 4.04GRR326 pKa = 11.84GDD328 pKa = 3.76ACDD331 pKa = 3.23VCPFRR336 pKa = 11.84RR337 pKa = 11.84PGDD340 pKa = 3.56TTGDD344 pKa = 3.95GIVDD348 pKa = 3.52GRR350 pKa = 11.84DD351 pKa = 2.98AQLFVRR357 pKa = 11.84ILTGGSGTPDD367 pKa = 3.22EE368 pKa = 4.7TCACDD373 pKa = 3.57VNEE376 pKa = 4.06DD377 pKa = 4.08TFVNEE382 pKa = 3.83ADD384 pKa = 3.33IPALVTLLLNQQ395 pKa = 3.7

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518LPJ3|A0A518LPJ3_9BACT ATPase family associated with various cellular activities (AAA) OS=Phycisphaerae bacterium RAS2 OX=2528035 GN=RAS2_29520 PE=4 SV=1
MM1 pKa = 7.78ISCQWPVISGQRR13 pKa = 11.84PLIGWHH19 pKa = 6.63GGGFWRR25 pKa = 11.84PSWSNVQPRR34 pKa = 11.84RR35 pKa = 11.84LVAGTDD41 pKa = 3.37SLIRR45 pKa = 11.84NATNGRR51 pKa = 11.84QSGVMRR57 pKa = 11.84FEE59 pKa = 4.67LLLPAARR66 pKa = 11.84MAIVIRR72 pKa = 11.84SVHH75 pKa = 4.87WLLRR79 pKa = 11.84RR80 pKa = 11.84LARR83 pKa = 11.84GRR85 pKa = 11.84INRR88 pKa = 11.84RR89 pKa = 11.84GLSAGARR96 pKa = 11.84SAAAVRR102 pKa = 11.84LGRR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84GRR109 pKa = 11.84GIAIHH114 pKa = 6.59RR115 pKa = 11.84RR116 pKa = 11.84TGARR120 pKa = 11.84GHH122 pKa = 5.82EE123 pKa = 4.07VRR125 pKa = 11.84QRR127 pKa = 11.84GVEE130 pKa = 3.83IARR133 pKa = 11.84RR134 pKa = 11.84AHH136 pKa = 5.4AVWRR140 pKa = 11.84RR141 pKa = 11.84SVGSLRR147 pKa = 11.84TALARR152 pKa = 11.84RR153 pKa = 11.84SITRR157 pKa = 11.84RR158 pKa = 11.84SAGADD163 pKa = 2.7IASITGCRR171 pKa = 11.84RR172 pKa = 11.84VARR175 pKa = 11.84RR176 pKa = 11.84GSIPRR181 pKa = 11.84WRR183 pKa = 11.84TISIAA188 pKa = 3.3

Molecular weight: 21.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3554

0

3554

1265709

29

4444

356.1

38.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.575 ± 0.053

1.337 ± 0.023

5.839 ± 0.035

5.579 ± 0.042

3.594 ± 0.026

8.212 ± 0.046

2.135 ± 0.019

4.934 ± 0.026

3.342 ± 0.039

9.57 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.348 ± 0.017

2.944 ± 0.033

5.463 ± 0.031

3.293 ± 0.022

7.579 ± 0.051

5.744 ± 0.027

5.43 ± 0.036

7.383 ± 0.033

1.409 ± 0.02

2.29 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski