Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus; Archaeoglobus fulgidus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O28620|O28620_ARCFU Alkaline serine protease (AprM) OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_1653 PE=4 SV=1
MM1 pKa = 8.59SEE3 pKa = 3.88MRR5 pKa = 11.84KK6 pKa = 9.55LLLSILSIGFMMAVIATGTLTYY28 pKa = 9.9FYY30 pKa = 10.73DD31 pKa = 4.8VEE33 pKa = 4.47VSDD36 pKa = 4.9NNTITAGTIDD46 pKa = 3.23ISVDD50 pKa = 3.48GEE52 pKa = 4.56NPWTTSVIVTDD63 pKa = 4.18DD64 pKa = 4.06AKK66 pKa = 10.92PSLNFCVYY74 pKa = 10.9KK75 pKa = 10.15LVCNNGTNPAVVYY88 pKa = 9.21TRR90 pKa = 11.84IDD92 pKa = 3.78NITEE96 pKa = 4.13SNNEE100 pKa = 3.65VTEE103 pKa = 4.43PEE105 pKa = 4.49LEE107 pKa = 4.35ADD109 pKa = 3.59PSGLINDD116 pKa = 4.96LSNVTWFDD124 pKa = 3.21LAYY127 pKa = 11.03NKK129 pKa = 11.0GEE131 pKa = 3.94GWNVLVKK138 pKa = 10.75EE139 pKa = 4.54GVLTVRR145 pKa = 11.84DD146 pKa = 4.22VEE148 pKa = 4.58GTWYY152 pKa = 10.22EE153 pKa = 4.21LTTLQPDD160 pKa = 3.3EE161 pKa = 4.5CVNVLFSFHH170 pKa = 6.27MKK172 pKa = 9.95SDD174 pKa = 3.31AGNEE178 pKa = 4.07YY179 pKa = 10.54QGDD182 pKa = 3.57QMQFDD187 pKa = 4.05IVFKK191 pKa = 10.79AFQVNDD197 pKa = 3.98DD198 pKa = 3.91GAPYY202 pKa = 10.76

Molecular weight: 22.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O28222|O28222_ARCFU Acyl-CoA dehydrogenase (Acd-10) OS=Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126) OX=224325 GN=AF_2057 PE=3 SV=1
MM1 pKa = 7.34GKK3 pKa = 8.36KK4 pKa = 8.33TVGVKK9 pKa = 10.09KK10 pKa = 10.45RR11 pKa = 11.84LAKK14 pKa = 10.15AYY16 pKa = 8.5KK17 pKa = 8.22QNRR20 pKa = 11.84RR21 pKa = 11.84APVWITVKK29 pKa = 9.6TKK31 pKa = 10.44RR32 pKa = 11.84SVFGSPKK39 pKa = 8.0RR40 pKa = 11.84RR41 pKa = 11.84HH42 pKa = 4.41WRR44 pKa = 11.84RR45 pKa = 11.84SKK47 pKa = 11.16LKK49 pKa = 9.99VV50 pKa = 3.04

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2394

0

2394

660812

25

2425

276.0

30.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.866 ± 0.049

1.178 ± 0.028

4.897 ± 0.038

8.921 ± 0.061

4.574 ± 0.039

7.255 ± 0.048

1.511 ± 0.021

7.229 ± 0.043

6.865 ± 0.05

9.498 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.619 ± 0.025

3.212 ± 0.037

3.86 ± 0.033

1.781 ± 0.02

5.765 ± 0.046

5.494 ± 0.045

4.166 ± 0.038

8.623 ± 0.047

1.039 ± 0.021

3.647 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski