Sulfolobales Mexican rudivirus 1

Taxonomy: Viruses; Adnaviria; Zilligvirae; Taleaviricota; Tokiviricetes; Ligamenvirales; Rudiviridae; Rudivirus; unclassified Rudivirus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4NZG9|K4NZG9_9VIRU Uncharacterized protein OS=Sulfolobales Mexican rudivirus 1 OX=1245909 PE=4 SV=1
MM1 pKa = 6.91VTLQQVSSVTVTSPWSDD18 pKa = 3.52YY19 pKa = 11.71NEE21 pKa = 4.24MPNQIQYY28 pKa = 11.16GSIVNPFYY36 pKa = 10.56PVFIFSYY43 pKa = 11.08VNTSNCGLWVVGYY56 pKa = 10.37VSGDD60 pKa = 3.17NAGVFFNHH68 pKa = 6.08ACGWVNVSGMFYY80 pKa = 10.52FDD82 pKa = 3.56AFGYY86 pKa = 8.81PYY88 pKa = 11.45AMGGGYY94 pKa = 8.98MSGVSSCANNGAVWWTFVNQKK115 pKa = 8.67TGSVNAYY122 pKa = 7.3CTGLIPNNDD131 pKa = 2.78VSMDD135 pKa = 4.5FISAVADD142 pKa = 3.9LNQQNILLLSLDD154 pKa = 3.41ISVMAFLIWIIPVSEE169 pKa = 4.35ISTLLNNSSPPSIKK183 pKa = 10.09FAVVKK188 pKa = 8.33TTDD191 pKa = 2.97VPNQYY196 pKa = 8.31MQQFFPVIYY205 pKa = 10.02DD206 pKa = 3.34GNLIIGSTYY215 pKa = 10.85DD216 pKa = 3.36YY217 pKa = 11.03VAYY220 pKa = 10.07LAVMPLSEE228 pKa = 4.3IYY230 pKa = 10.32VNAMTGIPTTNTPATYY246 pKa = 10.64VGTQYY251 pKa = 10.77TYY253 pKa = 10.99SEE255 pKa = 4.82YY256 pKa = 11.02EE257 pKa = 4.01FVTWPIITTIAIPSSGGLSIYY278 pKa = 10.22VSLMLGAKK286 pKa = 10.02NYY288 pKa = 10.64LLYY291 pKa = 10.24IANLSPSNWSVVSSFTFYY309 pKa = 10.41LTDD312 pKa = 3.37VASSICTAPVGSTWNGVFILMFNGPSSSCNTLYY345 pKa = 11.06VVVADD350 pKa = 4.67PKK352 pKa = 10.13SSNYY356 pKa = 9.88EE357 pKa = 3.5YY358 pKa = 10.34TYY360 pKa = 11.3INMTQNTDD368 pKa = 2.96IAILGYY374 pKa = 10.09EE375 pKa = 4.62GYY377 pKa = 10.55LVVSQQPLSDD387 pKa = 3.58STVTFVVYY395 pKa = 10.29QILLDD400 pKa = 3.57HH401 pKa = 6.78TYY403 pKa = 10.38VFQNVAVSTTSSTITFSGVLYY424 pKa = 10.54DD425 pKa = 4.06EE426 pKa = 4.66TAGAPVANATVWLVAVRR443 pKa = 11.84SYY445 pKa = 11.14VKK447 pKa = 10.29QYY449 pKa = 11.38SNDD452 pKa = 3.56VVPLASTTTDD462 pKa = 3.03SAGHH466 pKa = 5.15FTVSAPMQSGYY477 pKa = 10.25NAYY480 pKa = 9.96GVLYY484 pKa = 9.78TPP486 pKa = 5.2

Molecular weight: 52.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4NX87|K4NX87_9VIRU Putative SAM-dependent methyltransferase OS=Sulfolobales Mexican rudivirus 1 OX=1245909 PE=4 SV=1
MM1 pKa = 7.55HH2 pKa = 7.32NGAQKK7 pKa = 10.48RR8 pKa = 11.84VIKK11 pKa = 9.94LINVIRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 9.09KK21 pKa = 9.85PPAVRR26 pKa = 11.84TWIFHH31 pKa = 5.57YY32 pKa = 9.58IWHH35 pKa = 6.15GRR37 pKa = 11.84SYY39 pKa = 10.59TIATIAVRR47 pKa = 11.84LTGDD51 pKa = 3.32GALHH55 pKa = 5.25VRR57 pKa = 11.84KK58 pKa = 10.14KK59 pKa = 10.4IEE61 pKa = 3.9ARR63 pKa = 11.84LKK65 pKa = 10.32ALEE68 pKa = 4.35GVNVTTFLDD77 pKa = 3.75GEE79 pKa = 4.57YY80 pKa = 10.2IKK82 pKa = 10.47YY83 pKa = 8.51TLEE86 pKa = 3.86AA87 pKa = 4.73

Molecular weight: 10.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

7852

54

1106

212.2

24.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.221 ± 0.415

0.777 ± 0.177

4.648 ± 0.366

6.011 ± 0.711

4.661 ± 0.335

5.018 ± 0.347

1.617 ± 0.23

6.572 ± 0.36

5.183 ± 0.477

10.15 ± 0.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.177

5.323 ± 0.364

4.075 ± 0.346

4.483 ± 0.416

4.457 ± 0.498

6.597 ± 0.626

5.693 ± 0.347

8.482 ± 0.479

1.032 ± 0.186

5.604 ± 0.34
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski