Bacillus sp. MUM 13

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4336 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S2R8E1|A0A1S2R8E1_9BACI Uncharacterized protein OS=Bacillus sp. MUM 13 OX=1678001 GN=BIV59_04375 PE=4 SV=1
MM1 pKa = 7.52AKK3 pKa = 9.86IGVEE7 pKa = 3.6QSLANVQQALVEE19 pKa = 4.09KK20 pKa = 9.96GYY22 pKa = 11.22DD23 pKa = 3.47VVALNQEE30 pKa = 4.25SDD32 pKa = 3.29AQGCDD37 pKa = 3.31FCVVTGLDD45 pKa = 3.85SNVMGMQDD53 pKa = 3.24TSIKK57 pKa = 10.61GSVIDD62 pKa = 4.83ANGLSADD69 pKa = 4.1EE70 pKa = 4.24VCEE73 pKa = 3.94QIEE76 pKa = 4.56QKK78 pKa = 8.94MQYY81 pKa = 10.34

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S2R456|A0A1S2R456_9BACI Uncharacterized protein OS=Bacillus sp. MUM 13 OX=1678001 GN=BIV59_06455 PE=4 SV=1
MM1 pKa = 7.22PQDD4 pKa = 3.15AALLVFDD11 pKa = 4.91PLTRR15 pKa = 11.84RR16 pKa = 11.84LRR18 pKa = 11.84FFYY21 pKa = 10.42SIQLQRR27 pKa = 11.84LACCAKK33 pKa = 10.51DD34 pKa = 3.58VLASEE39 pKa = 4.6GKK41 pKa = 9.82EE42 pKa = 3.41RR43 pKa = 11.84LPRR46 pKa = 11.84PKK48 pKa = 10.17PYY50 pKa = 10.82LLRR53 pKa = 11.84KK54 pKa = 8.86LNRR57 pKa = 11.84RR58 pKa = 11.84FRR60 pKa = 11.84IFIHH64 pKa = 5.32EE65 pKa = 4.17TFLFYY70 pKa = 10.44IRR72 pKa = 11.84NNN74 pKa = 3.43

Molecular weight: 8.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4336

0

4336

1222119

26

1666

281.9

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.342 ± 0.039

0.757 ± 0.012

4.996 ± 0.031

7.249 ± 0.045

4.574 ± 0.03

7.084 ± 0.036

2.131 ± 0.017

7.965 ± 0.042

7.339 ± 0.037

9.785 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.787 ± 0.018

4.365 ± 0.024

3.624 ± 0.024

3.509 ± 0.024

4.044 ± 0.025

6.61 ± 0.028

5.03 ± 0.028

6.344 ± 0.029

0.99 ± 0.013

3.476 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski