Honeysuckle ringspot virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae; Alphacarmovirus

Average proteome isoelectric point is 8.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7EEK7|E7EEK7_9TOMB p13 OS=Honeysuckle ringspot virus OX=943272 GN=P13 PE=4 SV=1
MM1 pKa = 7.98DD2 pKa = 4.02VQPVEE7 pKa = 5.0AKK9 pKa = 10.47DD10 pKa = 3.49DD11 pKa = 3.61TRR13 pKa = 11.84IQSDD17 pKa = 3.81VEE19 pKa = 4.04RR20 pKa = 11.84GGNRR24 pKa = 11.84GKK26 pKa = 9.1TKK28 pKa = 10.49GRR30 pKa = 11.84ISIAKK35 pKa = 9.63DD36 pKa = 3.56AINKK40 pKa = 8.35RR41 pKa = 11.84ASDD44 pKa = 3.75GSIGGTYY51 pKa = 10.24YY52 pKa = 10.22IFADD56 pKa = 3.41KK57 pKa = 11.33VEE59 pKa = 4.18NTVNFNFGG67 pKa = 3.07

Molecular weight: 7.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7EEK6|E7EEK6_9TOMB p7 OS=Honeysuckle ringspot virus OX=943272 GN=P7 PE=4 SV=1
MM1 pKa = 7.6LLDD4 pKa = 3.87FCFKK8 pKa = 10.84AIIAPPIVGTVVAMGLAGLTVRR30 pKa = 11.84TTIGVVEE37 pKa = 4.99FEE39 pKa = 4.02ARR41 pKa = 11.84AVRR44 pKa = 11.84TVVDD48 pKa = 3.96YY49 pKa = 9.27ITSKK53 pKa = 11.13GKK55 pKa = 8.04TLPGKK60 pKa = 9.61PKK62 pKa = 10.07YY63 pKa = 9.82PKK65 pKa = 10.4AKK67 pKa = 9.83VNVFLEE73 pKa = 4.19EE74 pKa = 3.89LAEE77 pKa = 4.01VDD79 pKa = 3.34GDD81 pKa = 4.45DD82 pKa = 5.7RR83 pKa = 11.84EE84 pKa = 4.77LPDD87 pKa = 4.07LLEE90 pKa = 4.16TVEE93 pKa = 4.25VKK95 pKa = 10.82KK96 pKa = 10.96EE97 pKa = 3.9DD98 pKa = 3.86GEE100 pKa = 4.51GKK102 pKa = 9.22MVVVRR107 pKa = 11.84TKK109 pKa = 11.16AKK111 pKa = 10.06VNRR114 pKa = 11.84HH115 pKa = 3.35TRR117 pKa = 11.84GRR119 pKa = 11.84FVHH122 pKa = 6.73RR123 pKa = 11.84LVCATKK129 pKa = 9.85NHH131 pKa = 7.24LGGTPNNSTANKK143 pKa = 8.82LVAYY147 pKa = 9.34KK148 pKa = 10.29FMVGKK153 pKa = 10.32CKK155 pKa = 9.71EE156 pKa = 3.76QHH158 pKa = 6.01LVEE161 pKa = 4.11THH163 pKa = 4.56TRR165 pKa = 11.84EE166 pKa = 4.21VCSLAMAAIFTPDD179 pKa = 2.94VHH181 pKa = 6.87DD182 pKa = 4.34VNMHH186 pKa = 6.66LALNSHH192 pKa = 6.46AAYY195 pKa = 9.83RR196 pKa = 11.84RR197 pKa = 11.84RR198 pKa = 11.84VALAQAQEE206 pKa = 4.18VQSWQLRR213 pKa = 11.84LLKK216 pKa = 10.68HH217 pKa = 5.31PLKK220 pKa = 10.28YY221 pKa = 10.61DD222 pKa = 2.88SWEE225 pKa = 4.23RR226 pKa = 11.84AWLWANGAPTQEE238 pKa = 3.87PFRR241 pKa = 11.84FIKK244 pKa = 10.58

Molecular weight: 27.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2411

67

880

401.8

45.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.549 ± 0.407

1.783 ± 0.328

5.019 ± 0.408

5.641 ± 0.396

4.189 ± 0.281

6.139 ± 0.352

2.323 ± 0.387

4.687 ± 0.585

7.134 ± 0.487

8.752 ± 0.493

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.572 ± 0.195

3.982 ± 0.185

4.189 ± 0.334

3.899 ± 0.282

6.346 ± 0.169

5.35 ± 0.828

6.18 ± 0.963

9.083 ± 0.406

1.493 ± 0.068

3.567 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski