Streptomyces phage Jay2Jay

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Samistivirus; Streptomyces virus Jay2Jay

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0RL59|A0A0A0RL59_9CAUD Uncharacterized protein OS=Streptomyces phage Jay2Jay OX=1556290 GN=253 PE=4 SV=1
MM1 pKa = 7.76SYY3 pKa = 10.53QLQVLTDD10 pKa = 3.56APFSYY15 pKa = 9.79WKK17 pKa = 10.51LDD19 pKa = 3.37EE20 pKa = 4.85TGPAFPDD27 pKa = 3.27SAGSMRR33 pKa = 11.84TADD36 pKa = 3.72LVGTITRR43 pKa = 11.84HH44 pKa = 5.3PALVTGSGNALVLDD58 pKa = 4.24NTNHH62 pKa = 7.1LDD64 pKa = 3.3MDD66 pKa = 4.3DD67 pKa = 3.64PVFNKK72 pKa = 10.34GYY74 pKa = 8.92EE75 pKa = 3.89LRR77 pKa = 11.84QFSLEE82 pKa = 3.88AWVKK86 pKa = 8.55PVKK89 pKa = 9.53ITGEE93 pKa = 4.11VSVMSHH99 pKa = 5.65SGIYY103 pKa = 10.21DD104 pKa = 3.6GLTITPTHH112 pKa = 6.89IYY114 pKa = 10.58FSTKK118 pKa = 9.97YY119 pKa = 8.68LTAGSCEE126 pKa = 3.76VSYY129 pKa = 10.5EE130 pKa = 3.94YY131 pKa = 10.23STGKK135 pKa = 9.88SFHH138 pKa = 6.39VVGVHH143 pKa = 5.58TNAKK147 pKa = 9.52NSLYY151 pKa = 10.73VDD153 pKa = 4.01GVLVAEE159 pKa = 4.55TDD161 pKa = 3.59LTDD164 pKa = 3.45EE165 pKa = 4.24QQIDD169 pKa = 3.96SYY171 pKa = 12.04SFLTSDD177 pKa = 5.14LIAGQGSGTIALDD190 pKa = 3.54APAVYY195 pKa = 9.95TDD197 pKa = 3.73ALGAEE202 pKa = 4.95AISRR206 pKa = 11.84HH207 pKa = 5.2YY208 pKa = 10.94FNGVDD213 pKa = 3.35VDD215 pKa = 3.81TSEE218 pKa = 5.7AIAGFNDD225 pKa = 4.48AIFWNFTDD233 pKa = 3.48EE234 pKa = 4.21TRR236 pKa = 11.84NIAIKK241 pKa = 10.36KK242 pKa = 8.77VWASDD247 pKa = 4.22DD248 pKa = 3.7DD249 pKa = 3.81WSDD252 pKa = 3.52AVLTDD257 pKa = 3.2VAVIDD262 pKa = 4.07GTIVPSYY269 pKa = 10.41SQTEE273 pKa = 4.39TEE275 pKa = 4.26VVEE278 pKa = 5.07DD279 pKa = 4.01GLIVPVYY286 pKa = 10.23EE287 pKa = 4.19NTSLPGVWLASLSFTSIPEE306 pKa = 3.85EE307 pKa = 4.27TITDD311 pKa = 3.48ARR313 pKa = 11.84INWVGEE319 pKa = 3.63GSYY322 pKa = 10.12TIEE325 pKa = 4.06YY326 pKa = 10.3SVDD329 pKa = 3.25GGNIWLEE336 pKa = 4.16PSDD339 pKa = 4.15SGATVLDD346 pKa = 3.83NEE348 pKa = 4.55SVIDD352 pKa = 3.77IRR354 pKa = 11.84VTFDD358 pKa = 2.89GGIVDD363 pKa = 4.97DD364 pKa = 4.7EE365 pKa = 5.2SFVSSIEE372 pKa = 3.99VVGYY376 pKa = 9.97LDD378 pKa = 3.0KK379 pKa = 11.11YY380 pKa = 10.03YY381 pKa = 10.6RR382 pKa = 11.84GSRR385 pKa = 11.84ADD387 pKa = 3.39RR388 pKa = 11.84TAQMSGTGVVSSSYY402 pKa = 10.69FEE404 pKa = 4.97PIEE407 pKa = 4.0YY408 pKa = 10.74ADD410 pKa = 3.7VNGLRR415 pKa = 11.84IITGTVNVSLDD426 pKa = 3.37DD427 pKa = 4.06SYY429 pKa = 11.88EE430 pKa = 4.32GEE432 pKa = 4.43QEE434 pKa = 4.84AGDD437 pKa = 4.2LNVNGIDD444 pKa = 3.4MWIKK448 pKa = 8.59PTVGNILTATGISISRR464 pKa = 11.84VGNTIVFSGFSSFTVNGNSVTSGDD488 pKa = 3.71TVFDD492 pKa = 3.58SDD494 pKa = 3.26SWYY497 pKa = 10.53HH498 pKa = 5.08IAGVFSTPGNYY509 pKa = 10.43AMSIASAGTIISQLSVIYY527 pKa = 10.48GSLSLLGLQQIYY539 pKa = 10.17AAYY542 pKa = 10.17LGLPGLIVNDD552 pKa = 3.96TSTVDD557 pKa = 3.04IGEE560 pKa = 4.53GSPATNLYY568 pKa = 9.27AHH570 pKa = 6.59VWGITPAGG578 pKa = 3.48

Molecular weight: 61.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0RT10|A0A0A0RT10_9CAUD Uncharacterized protein OS=Streptomyces phage Jay2Jay OX=1556290 GN=51 PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 9.78TDD4 pKa = 3.35EE5 pKa = 4.86DD6 pKa = 3.93RR7 pKa = 11.84CIEE10 pKa = 4.13YY11 pKa = 10.35GVPYY15 pKa = 9.83DD16 pKa = 3.79PSITRR21 pKa = 11.84PVVYY25 pKa = 10.03AQSNWTCHH33 pKa = 5.64LCGKK37 pKa = 9.81RR38 pKa = 11.84VSKK41 pKa = 10.3RR42 pKa = 11.84LKK44 pKa = 9.48YY45 pKa = 8.65PHH47 pKa = 7.26PKK49 pKa = 9.85SASLDD54 pKa = 3.89HH55 pKa = 6.64IVPLSWRR62 pKa = 11.84KK63 pKa = 9.93DD64 pKa = 3.68SPGHH68 pKa = 4.45VWGNVALAHH77 pKa = 6.12LRR79 pKa = 11.84CNQSKK84 pKa = 9.12GARR87 pKa = 11.84FAGSTRR93 pKa = 11.84PAPRR97 pKa = 11.84RR98 pKa = 11.84PGFINNVWKK107 pKa = 10.6LRR109 pKa = 11.84IALFGFTGAMFYY121 pKa = 10.71FGATPLALTIAVALCILSVIKK142 pKa = 10.51VKK144 pKa = 10.53KK145 pKa = 8.25SRR147 pKa = 11.84RR148 pKa = 11.84RR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84AWWKK156 pKa = 10.21LL157 pKa = 3.23

Molecular weight: 17.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

209

0

209

34582

23

2103

165.5

18.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.444 ± 0.379

0.992 ± 0.11

6.48 ± 0.179

6.833 ± 0.299

3.831 ± 0.12

7.504 ± 0.177

1.877 ± 0.128

5.384 ± 0.149

6.252 ± 0.278

7.226 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.903 ± 0.118

4.572 ± 0.15

3.803 ± 0.177

3.279 ± 0.186

5.584 ± 0.182

6.13 ± 0.248

6.171 ± 0.314

7.145 ± 0.209

1.885 ± 0.094

3.704 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski