Amino acid dipepetide frequency for Nitrosopumilus sp. PRT-SC01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.8AlaAla: 4.8 ± 0.285
0.63AlaCys: 0.63 ± 0.068
2.942AlaAsp: 2.942 ± 0.135
3.836AlaGlu: 3.836 ± 0.163
2.463AlaPhe: 2.463 ± 0.126
4.094AlaGly: 4.094 ± 0.172
0.995AlaHis: 0.995 ± 0.076
5.826AlaIle: 5.826 ± 0.228
6.022AlaLys: 6.022 ± 0.192
5.663AlaLeu: 5.663 ± 0.206
1.505AlaMet: 1.505 ± 0.081
2.362AlaAsn: 2.362 ± 0.132
1.757AlaPro: 1.757 ± 0.11
1.757AlaGln: 1.757 ± 0.101
2.375AlaArg: 2.375 ± 0.128
3.924AlaSer: 3.924 ± 0.182
3.149AlaThr: 3.149 ± 0.15
3.786AlaVal: 3.786 ± 0.177
0.46AlaTrp: 0.46 ± 0.049
1.732AlaTyr: 1.732 ± 0.118
0.0AlaXaa: 0.0 ± 0.0
Cys
0.586CysAla: 0.586 ± 0.057
0.132CysCys: 0.132 ± 0.03
0.579CysAsp: 0.579 ± 0.066
0.579CysGlu: 0.579 ± 0.069
0.447CysPhe: 0.447 ± 0.055
1.008CysGly: 1.008 ± 0.082
0.17CysHis: 0.17 ± 0.031
0.894CysIle: 0.894 ± 0.077
0.976CysLys: 0.976 ± 0.087
0.674CysLeu: 0.674 ± 0.08
0.246CysMet: 0.246 ± 0.04
0.63CysAsn: 0.63 ± 0.062
0.535CysPro: 0.535 ± 0.064
0.302CysGln: 0.302 ± 0.045
0.46CysArg: 0.46 ± 0.051
0.768CysSer: 0.768 ± 0.075
0.542CysThr: 0.542 ± 0.068
0.548CysVal: 0.548 ± 0.059
0.082CysTrp: 0.082 ± 0.023
0.391CysTyr: 0.391 ± 0.047
0.0CysXaa: 0.0 ± 0.0
Asp
3.502AspAla: 3.502 ± 0.149
0.567AspCys: 0.567 ± 0.06
3.061AspAsp: 3.061 ± 0.169
4.434AspGlu: 4.434 ± 0.164
2.797AspPhe: 2.797 ± 0.151
3.477AspGly: 3.477 ± 0.202
0.794AspHis: 0.794 ± 0.079
4.724AspIle: 4.724 ± 0.207
4.239AspLys: 4.239 ± 0.162
4.976AspLeu: 4.976 ± 0.16
1.575AspMet: 1.575 ± 0.1
2.148AspAsn: 2.148 ± 0.115
2.268AspPro: 2.268 ± 0.107
1.361AspGln: 1.361 ± 0.072
1.814AspArg: 1.814 ± 0.121
4.271AspSer: 4.271 ± 0.175
2.431AspThr: 2.431 ± 0.124
4.308AspVal: 4.308 ± 0.166
0.529AspTrp: 0.529 ± 0.063
2.047AspTyr: 2.047 ± 0.132
0.0AspXaa: 0.0 ± 0.0
Glu
3.653GluAla: 3.653 ± 0.164
0.649GluCys: 0.649 ± 0.059
3.263GluAsp: 3.263 ± 0.151
4.976GluGlu: 4.976 ± 0.216
3.408GluPhe: 3.408 ± 0.144
3.552GluGly: 3.552 ± 0.172
1.197GluHis: 1.197 ± 0.082
7.955GluIle: 7.955 ± 0.219
8.069GluLys: 8.069 ± 0.275
6.425GluLeu: 6.425 ± 0.232
2.009GluMet: 2.009 ± 0.112
4.138GluAsn: 4.138 ± 0.166
2.286GluPro: 2.286 ± 0.172
2.394GluGln: 2.394 ± 0.113
2.375GluArg: 2.375 ± 0.127
4.869GluSer: 4.869 ± 0.164
3.326GluThr: 3.326 ± 0.14
3.647GluVal: 3.647 ± 0.154
0.68GluTrp: 0.68 ± 0.068
2.305GluTyr: 2.305 ± 0.106
0.0GluXaa: 0.0 ± 0.0
Phe
2.778PheAla: 2.778 ± 0.148
0.592PheCys: 0.592 ± 0.054
3.301PheAsp: 3.301 ± 0.169
3.143PheGlu: 3.143 ± 0.128
1.814PhePhe: 1.814 ± 0.127
3.364PheGly: 3.364 ± 0.155
0.831PheHis: 0.831 ± 0.074
2.74PheIle: 2.74 ± 0.144
2.816PheLys: 2.816 ± 0.126
3.685PheLeu: 3.685 ± 0.147
1.027PheMet: 1.027 ± 0.085
1.852PheAsn: 1.852 ± 0.109
1.587PhePro: 1.587 ± 0.088
1.304PheGln: 1.304 ± 0.082
1.449PheArg: 1.449 ± 0.107
3.496PheSer: 3.496 ± 0.153
2.23PheThr: 2.23 ± 0.138
3.345PheVal: 3.345 ± 0.155
0.416PheTrp: 0.416 ± 0.052
1.424PheTyr: 1.424 ± 0.098
0.0PheXaa: 0.0 ± 0.0
Gly
4.138GlyAla: 4.138 ± 0.187
0.598GlyCys: 0.598 ± 0.055
3.187GlyAsp: 3.187 ± 0.136
3.559GlyGlu: 3.559 ± 0.156
3.181GlyPhe: 3.181 ± 0.144
4.875GlyGly: 4.875 ± 0.25
1.266GlyHis: 1.266 ± 0.086
7.244GlyIle: 7.244 ± 0.201
6.047GlyLys: 6.047 ± 0.206
5.543GlyLeu: 5.543 ± 0.233
1.946GlyMet: 1.946 ± 0.134
2.847GlyAsn: 2.847 ± 0.12
1.732GlyPro: 1.732 ± 0.104
1.688GlyGln: 1.688 ± 0.116
2.57GlyArg: 2.57 ± 0.129
4.044GlySer: 4.044 ± 0.178
3.496GlyThr: 3.496 ± 0.17
4.453GlyVal: 4.453 ± 0.169
0.573GlyTrp: 0.573 ± 0.061
2.538GlyTyr: 2.538 ± 0.128
0.0GlyXaa: 0.0 ± 0.0
His
1.033HisAla: 1.033 ± 0.087
0.208HisCys: 0.208 ± 0.034
1.033HisAsp: 1.033 ± 0.074
1.153HisGlu: 1.153 ± 0.079
0.863HisPhe: 0.863 ± 0.08
1.247HisGly: 1.247 ± 0.077
0.435HisHis: 0.435 ± 0.054
1.253HisIle: 1.253 ± 0.071
1.134HisLys: 1.134 ± 0.089
1.581HisLeu: 1.581 ± 0.109
0.485HisMet: 0.485 ± 0.062
0.882HisAsn: 0.882 ± 0.081
0.926HisPro: 0.926 ± 0.064
0.529HisGln: 0.529 ± 0.058
0.806HisArg: 0.806 ± 0.082
1.083HisSer: 1.083 ± 0.084
1.14HisThr: 1.14 ± 0.077
1.102HisVal: 1.102 ± 0.081
0.164HisTrp: 0.164 ± 0.03
0.68HisTyr: 0.68 ± 0.065
0.0HisXaa: 0.0 ± 0.0
Ile
6.311IleAla: 6.311 ± 0.202
0.876IleCys: 0.876 ± 0.068
5.234IleAsp: 5.234 ± 0.174
6.689IleGlu: 6.689 ± 0.216
3.59IlePhe: 3.59 ± 0.148
6.274IleGly: 6.274 ± 0.236
1.688IleHis: 1.688 ± 0.119
8.125IleIle: 8.125 ± 0.241
7.521IleLys: 7.521 ± 0.224
8.207IleLeu: 8.207 ± 0.259
1.978IleMet: 1.978 ± 0.089
4.176IleAsn: 4.176 ± 0.164
4.208IlePro: 4.208 ± 0.143
3.156IleGln: 3.156 ± 0.15
3.319IleArg: 3.319 ± 0.154
7.319IleSer: 7.319 ± 0.213
5.562IleThr: 5.562 ± 0.172
5.984IleVal: 5.984 ± 0.199
0.699IleTrp: 0.699 ± 0.063
2.135IleTyr: 2.135 ± 0.117
0.0IleXaa: 0.0 ± 0.0
Lys
4.63LysAla: 4.63 ± 0.167
0.913LysCys: 0.913 ± 0.09
4.749LysAsp: 4.749 ± 0.157
6.551LysGlu: 6.551 ± 0.198
3.754LysPhe: 3.754 ± 0.171
4.472LysGly: 4.472 ± 0.186
1.487LysHis: 1.487 ± 0.091
10.204LysIle: 10.204 ± 0.306
9.895LysLys: 9.895 ± 0.335
7.445LysLeu: 7.445 ± 0.275
2.727LysMet: 2.727 ± 0.152
5.537LysAsn: 5.537 ± 0.207
2.822LysPro: 2.822 ± 0.123
3.137LysGln: 3.137 ± 0.161
3.301LysArg: 3.301 ± 0.149
5.82LysSer: 5.82 ± 0.18
5.096LysThr: 5.096 ± 0.213
4.718LysVal: 4.718 ± 0.18
0.756LysTrp: 0.756 ± 0.072
2.784LysTyr: 2.784 ± 0.143
0.0LysXaa: 0.0 ± 0.0
Leu
5.53LeuAla: 5.53 ± 0.199
0.762LeuCys: 0.762 ± 0.076
5.121LeuAsp: 5.121 ± 0.179
7.288LeuGlu: 7.288 ± 0.228
3.357LeuPhe: 3.357 ± 0.159
5.814LeuGly: 5.814 ± 0.165
1.48LeuHis: 1.48 ± 0.095
6.966LeuIle: 6.966 ± 0.219
8.207LeuLys: 8.207 ± 0.282
7.01LeuLeu: 7.01 ± 0.229
2.06LeuMet: 2.06 ± 0.11
3.723LeuAsn: 3.723 ± 0.148
3.112LeuPro: 3.112 ± 0.129
2.891LeuGln: 2.891 ± 0.128
3.471LeuArg: 3.471 ± 0.164
6.406LeuSer: 6.406 ± 0.214
4.453LeuThr: 4.453 ± 0.182
5.952LeuVal: 5.952 ± 0.229
0.605LeuTrp: 0.605 ± 0.069
2.589LeuTyr: 2.589 ± 0.146
0.0LeuXaa: 0.0 ± 0.0
Met
1.751MetAla: 1.751 ± 0.115
0.202MetCys: 0.202 ± 0.035
1.279MetAsp: 1.279 ± 0.084
1.556MetGlu: 1.556 ± 0.11
1.039MetPhe: 1.039 ± 0.073
1.978MetGly: 1.978 ± 0.136
0.428MetHis: 0.428 ± 0.047
2.469MetIle: 2.469 ± 0.142
2.595MetLys: 2.595 ± 0.126
2.268MetLeu: 2.268 ± 0.127
0.825MetMet: 0.825 ± 0.073
1.203MetAsn: 1.203 ± 0.097
0.983MetPro: 0.983 ± 0.09
0.85MetGln: 0.85 ± 0.077
0.945MetArg: 0.945 ± 0.083
1.808MetSer: 1.808 ± 0.095
1.644MetThr: 1.644 ± 0.101
1.549MetVal: 1.549 ± 0.104
0.164MetTrp: 0.164 ± 0.037
0.768MetTyr: 0.768 ± 0.07
0.0MetXaa: 0.0 ± 0.0
Asn
2.633AsnAla: 2.633 ± 0.132
0.661AsnCys: 0.661 ± 0.069
2.645AsnAsp: 2.645 ± 0.123
3.414AsnGlu: 3.414 ± 0.131
2.438AsnPhe: 2.438 ± 0.124
2.639AsnGly: 2.639 ± 0.138
1.039AsnHis: 1.039 ± 0.083
4.082AsnIle: 4.082 ± 0.165
3.546AsnLys: 3.546 ± 0.15
4.63AsnLeu: 4.63 ± 0.156
1.411AsnMet: 1.411 ± 0.099
2.173AsnAsn: 2.173 ± 0.131
2.324AsnPro: 2.324 ± 0.134
1.606AsnGln: 1.606 ± 0.108
1.732AsnArg: 1.732 ± 0.092
3.534AsnSer: 3.534 ± 0.144
2.608AsnThr: 2.608 ± 0.134
3.055AsnVal: 3.055 ± 0.124
0.523AsnTrp: 0.523 ± 0.056
1.877AsnTyr: 1.877 ± 0.116
0.0AsnXaa: 0.0 ± 0.0
Pro
1.713ProAla: 1.713 ± 0.108
0.265ProCys: 0.265 ± 0.045
2.186ProAsp: 2.186 ± 0.123
3.275ProGlu: 3.275 ± 0.163
1.657ProPhe: 1.657 ± 0.091
2.299ProGly: 2.299 ± 0.132
0.743ProHis: 0.743 ± 0.07
3.187ProIle: 3.187 ± 0.146
2.973ProLys: 2.973 ± 0.139
2.816ProLeu: 2.816 ± 0.139
0.857ProMet: 0.857 ± 0.073
1.852ProAsn: 1.852 ± 0.1
1.039ProPro: 1.039 ± 0.091
1.178ProGln: 1.178 ± 0.089
1.109ProArg: 1.109 ± 0.084
2.242ProSer: 2.242 ± 0.119
2.154ProThr: 2.154 ± 0.137
2.412ProVal: 2.412 ± 0.131
0.302ProTrp: 0.302 ± 0.048
1.235ProTyr: 1.235 ± 0.08
0.0ProXaa: 0.0 ± 0.0
Gln
1.631GlnAla: 1.631 ± 0.1
0.309GlnCys: 0.309 ± 0.052
1.442GlnAsp: 1.442 ± 0.076
2.186GlnGlu: 2.186 ± 0.115
1.361GlnPhe: 1.361 ± 0.094
1.701GlnGly: 1.701 ± 0.098
0.542GlnHis: 0.542 ± 0.063
3.433GlnIle: 3.433 ± 0.131
3.496GlnLys: 3.496 ± 0.151
2.519GlnLeu: 2.519 ± 0.137
0.97GlnMet: 0.97 ± 0.085
1.738GlnAsn: 1.738 ± 0.106
0.724GlnPro: 0.724 ± 0.072
0.92GlnGln: 0.92 ± 0.088
1.052GlnArg: 1.052 ± 0.092
1.871GlnSer: 1.871 ± 0.118
1.858GlnThr: 1.858 ± 0.106
2.009GlnVal: 2.009 ± 0.113
0.214GlnTrp: 0.214 ± 0.039
0.92GlnTyr: 0.92 ± 0.078
0.0GlnXaa: 0.0 ± 0.0
Arg
1.997ArgAla: 1.997 ± 0.128
0.479ArgCys: 0.479 ± 0.053
2.085ArgAsp: 2.085 ± 0.124
2.734ArgGlu: 2.734 ± 0.151
1.468ArgPhe: 1.468 ± 0.096
2.312ArgGly: 2.312 ± 0.145
0.668ArgHis: 0.668 ± 0.065
3.552ArgIle: 3.552 ± 0.156
3.716ArgLys: 3.716 ± 0.153
3.376ArgLeu: 3.376 ± 0.149
0.989ArgMet: 0.989 ± 0.084
1.795ArgAsn: 1.795 ± 0.092
1.247ArgPro: 1.247 ± 0.09
1.033ArgGln: 1.033 ± 0.074
1.65ArgArg: 1.65 ± 0.127
2.085ArgSer: 2.085 ± 0.128
1.997ArgThr: 1.997 ± 0.133
2.167ArgVal: 2.167 ± 0.112
0.378ArgTrp: 0.378 ± 0.057
1.184ArgTyr: 1.184 ± 0.083
0.0ArgXaa: 0.0 ± 0.0
Ser
3.804SerAla: 3.804 ± 0.156
0.693SerCys: 0.693 ± 0.069
4.031SerAsp: 4.031 ± 0.173
5.096SerGlu: 5.096 ± 0.218
2.879SerPhe: 2.879 ± 0.119
4.793SerGly: 4.793 ± 0.198
1.071SerHis: 1.071 ± 0.083
6.337SerIle: 6.337 ± 0.193
6.544SerLys: 6.544 ± 0.258
6.154SerLeu: 6.154 ± 0.16
1.839SerMet: 1.839 ± 0.113
3.37SerAsn: 3.37 ± 0.148
2.085SerPro: 2.085 ± 0.097
2.28SerGln: 2.28 ± 0.116
2.683SerArg: 2.683 ± 0.126
4.894SerSer: 4.894 ± 0.183
3.672SerThr: 3.672 ± 0.148
4.787SerVal: 4.787 ± 0.17
0.598SerTrp: 0.598 ± 0.06
2.009SerTyr: 2.009 ± 0.103
0.0SerXaa: 0.0 ± 0.0
Thr
3.149ThrAla: 3.149 ± 0.148
0.611ThrCys: 0.611 ± 0.069
2.664ThrAsp: 2.664 ± 0.117
3.716ThrGlu: 3.716 ± 0.14
2.072ThrPhe: 2.072 ± 0.105
4.176ThrGly: 4.176 ± 0.183
0.92ThrHis: 0.92 ± 0.082
5.373ThrIle: 5.373 ± 0.194
4.774ThrLys: 4.774 ± 0.189
4.85ThrLeu: 4.85 ± 0.154
1.361ThrMet: 1.361 ± 0.09
2.809ThrAsn: 2.809 ± 0.129
2.186ThrPro: 2.186 ± 0.12
1.631ThrGln: 1.631 ± 0.104
1.839ThrArg: 1.839 ± 0.097
3.647ThrSer: 3.647 ± 0.172
3.08ThrThr: 3.08 ± 0.17
3.294ThrVal: 3.294 ± 0.174
0.491ThrTrp: 0.491 ± 0.056
1.499ThrTyr: 1.499 ± 0.094
0.0ThrXaa: 0.0 ± 0.0
Val
4.082ValAla: 4.082 ± 0.171
0.838ValCys: 0.838 ± 0.071
3.71ValAsp: 3.71 ± 0.156
4.46ValGlu: 4.46 ± 0.185
2.809ValPhe: 2.809 ± 0.159
4.604ValGly: 4.604 ± 0.178
1.115ValHis: 1.115 ± 0.086
5.965ValIle: 5.965 ± 0.21
5.196ValLys: 5.196 ± 0.175
5.304ValLeu: 5.304 ± 0.199
1.713ValMet: 1.713 ± 0.109
3.256ValAsn: 3.256 ± 0.149
2.135ValPro: 2.135 ± 0.132
1.436ValGln: 1.436 ± 0.093
2.337ValArg: 2.337 ± 0.11
4.674ValSer: 4.674 ± 0.167
3.741ValThr: 3.741 ± 0.167
4.163ValVal: 4.163 ± 0.208
0.491ValTrp: 0.491 ± 0.054
1.902ValTyr: 1.902 ± 0.117
0.0ValXaa: 0.0 ± 0.0
Trp
0.51TrpAla: 0.51 ± 0.062
0.076TrpCys: 0.076 ± 0.019
0.523TrpAsp: 0.523 ± 0.057
0.403TrpGlu: 0.403 ± 0.054
0.441TrpPhe: 0.441 ± 0.045
0.624TrpGly: 0.624 ± 0.061
0.22TrpHis: 0.22 ± 0.042
0.687TrpIle: 0.687 ± 0.072
0.85TrpLys: 0.85 ± 0.074
0.687TrpLeu: 0.687 ± 0.069
0.202TrpMet: 0.202 ± 0.033
0.63TrpAsn: 0.63 ± 0.07
0.239TrpPro: 0.239 ± 0.042
0.346TrpGln: 0.346 ± 0.057
0.34TrpArg: 0.34 ± 0.041
0.466TrpSer: 0.466 ± 0.052
0.428TrpThr: 0.428 ± 0.049
0.542TrpVal: 0.542 ± 0.061
0.132TrpTrp: 0.132 ± 0.029
0.214TrpTyr: 0.214 ± 0.042
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.846TyrAla: 1.846 ± 0.126
0.491TyrCys: 0.491 ± 0.062
2.286TyrAsp: 2.286 ± 0.117
2.129TyrGlu: 2.129 ± 0.11
1.354TyrPhe: 1.354 ± 0.098
2.223TyrGly: 2.223 ± 0.125
0.649TyrHis: 0.649 ± 0.065
2.034TyrIle: 2.034 ± 0.114
2.179TyrLys: 2.179 ± 0.106
2.986TyrLeu: 2.986 ± 0.164
0.573TyrMet: 0.573 ± 0.055
1.455TyrAsn: 1.455 ± 0.099
1.298TyrPro: 1.298 ± 0.091
1.096TyrGln: 1.096 ± 0.083
1.285TyrArg: 1.285 ± 0.095
2.4TyrSer: 2.4 ± 0.114
1.537TyrThr: 1.537 ± 0.095
2.11TyrVal: 2.11 ± 0.117
0.302TyrTrp: 0.302 ± 0.049
1.115TyrTyr: 1.115 ± 0.093
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 709 proteins (158763 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski