Streptococcus phage IPP25

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5SA96|A0A1S5SA96_9CAUD Uncharacterized protein OS=Streptococcus phage IPP25 OX=1916165 GN=IPP25_00024 PE=4 SV=1
MM1 pKa = 7.61SYY3 pKa = 11.03DD4 pKa = 3.93LEE6 pKa = 4.79ILAKK10 pKa = 10.0IEE12 pKa = 4.16SGDD15 pKa = 3.93YY16 pKa = 10.41ICIAEE21 pKa = 4.4PRR23 pKa = 11.84YY24 pKa = 10.33SSPTYY29 pKa = 10.76NLGKK33 pKa = 9.37MFRR36 pKa = 11.84IAMNWDD42 pKa = 3.45FDD44 pKa = 3.75QDD46 pKa = 3.51TTYY49 pKa = 11.23NIADD53 pKa = 3.65ILDD56 pKa = 4.18NIQRR60 pKa = 11.84GISEE64 pKa = 4.42LEE66 pKa = 4.01RR67 pKa = 11.84YY68 pKa = 8.22PEE70 pKa = 4.56KK71 pKa = 10.66YY72 pKa = 9.5VQYY75 pKa = 10.07EE76 pKa = 3.96PEE78 pKa = 4.16NRR80 pKa = 11.84WGTVSGALEE89 pKa = 3.92VLKK92 pKa = 10.78SLKK95 pKa = 10.35EE96 pKa = 4.23CILEE100 pKa = 3.7QDD102 pKa = 2.84IDD104 pKa = 3.77MKK106 pKa = 10.43YY107 pKa = 11.0LYY109 pKa = 9.5MRR111 pKa = 11.84WW112 pKa = 3.31

Molecular weight: 13.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5SAF7|A0A1S5SAF7_9CAUD Lysozyme OS=Streptococcus phage IPP25 OX=1916165 GN=IPP25_00053 PE=3 SV=1
MM1 pKa = 7.3PQSLCWKK8 pKa = 10.12NEE10 pKa = 3.57YY11 pKa = 9.05TEE13 pKa = 4.21YY14 pKa = 9.88MHH16 pKa = 6.9EE17 pKa = 4.1ICPGRR22 pKa = 11.84LTPEE26 pKa = 3.75VTRR29 pKa = 11.84LLNEE33 pKa = 4.12KK34 pKa = 10.51FGTNYY39 pKa = 10.57NKK41 pKa = 8.98TQIGEE46 pKa = 3.91VRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84LGLPVGKK57 pKa = 10.29VYY59 pKa = 10.35QGKK62 pKa = 9.17LLTKK66 pKa = 9.08EE67 pKa = 3.64QHH69 pKa = 6.9DD70 pKa = 4.5YY71 pKa = 11.1LVSIQKK77 pKa = 10.33NKK79 pKa = 9.84ISRR82 pKa = 11.84DD83 pKa = 3.24VANEE87 pKa = 3.67MNQKK91 pKa = 10.35FGLSLTEE98 pKa = 4.06KK99 pKa = 10.22QIKK102 pKa = 9.48SYY104 pKa = 10.67RR105 pKa = 11.84RR106 pKa = 11.84NNNLHH111 pKa = 6.24SGLTGRR117 pKa = 11.84FEE119 pKa = 5.07KK120 pKa = 10.99GRR122 pKa = 11.84TPHH125 pKa = 6.57NKK127 pKa = 8.87GKK129 pKa = 10.47KK130 pKa = 8.0YY131 pKa = 10.86PNMPKK136 pKa = 10.38NSGQFKK142 pKa = 10.28KK143 pKa = 10.93GNRR146 pKa = 11.84PPNYY150 pKa = 9.98VPVGTINYY158 pKa = 7.06TTDD161 pKa = 3.55GYY163 pKa = 9.73PKK165 pKa = 10.44EE166 pKa = 4.95KK167 pKa = 9.75IGEE170 pKa = 4.09PNQWVLKK177 pKa = 9.54HH178 pKa = 5.71RR179 pKa = 11.84KK180 pKa = 8.04VWEE183 pKa = 4.15DD184 pKa = 2.87HH185 pKa = 6.76HH186 pKa = 8.25GPIPKK191 pKa = 9.99GYY193 pKa = 10.33SIVFLDD199 pKa = 4.36GDD201 pKa = 3.75KK202 pKa = 11.06TNYY205 pKa = 10.28DD206 pKa = 3.57ISNLACLSKK215 pKa = 11.52NEE217 pKa = 4.07IARR220 pKa = 11.84MNQNHH225 pKa = 7.16LFTSNADD232 pKa = 3.59LTKK235 pKa = 10.82SGIGLTKK242 pKa = 9.48LTNKK246 pKa = 9.28IRR248 pKa = 11.84EE249 pKa = 4.16VEE251 pKa = 4.19KK252 pKa = 11.28NGG254 pKa = 3.57

Molecular weight: 29.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10701

50

873

205.8

23.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.27 ± 0.381

0.495 ± 0.076

6.411 ± 0.291

7.868 ± 0.344

4.121 ± 0.233

5.925 ± 0.43

1.42 ± 0.136

7.429 ± 0.276

8.85 ± 0.419

8.672 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.589 ± 0.171

6.168 ± 0.249

2.598 ± 0.163

3.701 ± 0.213

4.233 ± 0.376

5.943 ± 0.296

6.149 ± 0.235

5.897 ± 0.246

1.308 ± 0.178

3.953 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski