Leisingera sp. ANG1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Leisingera; unclassified Leisingera

Average proteome isoelectric point is 6.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4159 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4EAY3|A0A0B4EAY3_9RHOB Uncharacterized protein OS=Leisingera sp. ANG1 OX=1002340 GN=GC1_00830 PE=4 SV=1
MM1 pKa = 7.66PSGYY5 pKa = 10.19LVQLGDD11 pKa = 3.46GSLDD15 pKa = 3.4HH16 pKa = 7.36GDD18 pKa = 4.5TITSTYY24 pKa = 11.37ASFTTDD30 pKa = 2.92QNLGAGNWSWTGSHH44 pKa = 6.71GGTPYY49 pKa = 10.61TNEE52 pKa = 4.27LEE54 pKa = 4.31PGVYY58 pKa = 10.42YY59 pKa = 10.92LATDD63 pKa = 3.73GNVYY67 pKa = 9.72FVPDD71 pKa = 4.14YY72 pKa = 11.06GPPDD76 pKa = 3.81TFSSASADD84 pKa = 3.63NPPAYY89 pKa = 10.24SDD91 pKa = 3.03TDD93 pKa = 3.8GAVEE97 pKa = 4.3GTSGADD103 pKa = 3.71TIDD106 pKa = 3.53SSYY109 pKa = 11.26TDD111 pKa = 3.94ADD113 pKa = 3.56GDD115 pKa = 4.59QIDD118 pKa = 3.95NGNGGGASGNQDD130 pKa = 3.08TVEE133 pKa = 4.5AGAGDD138 pKa = 3.91DD139 pKa = 4.13TVDD142 pKa = 3.7AGADD146 pKa = 3.13NDD148 pKa = 4.04MVFGGEE154 pKa = 4.54GGDD157 pKa = 3.81SLAGGSGDD165 pKa = 3.41DD166 pKa = 4.1TIYY169 pKa = 11.25GDD171 pKa = 3.83SSSEE175 pKa = 4.01AGLTDD180 pKa = 3.52SDD182 pKa = 5.18QSITDD187 pKa = 3.67ANVTDD192 pKa = 3.95TSSGFTVTAKK202 pKa = 9.66TVAGNTSAANIDD214 pKa = 4.32YY215 pKa = 10.78YY216 pKa = 11.47DD217 pKa = 3.63GGYY220 pKa = 10.33GVAGAISDD228 pKa = 3.82SDD230 pKa = 3.92SGVDD234 pKa = 3.67AQIGYY239 pKa = 10.13DD240 pKa = 3.28KK241 pKa = 11.3ASGVSEE247 pKa = 3.94EE248 pKa = 5.17LIVDD252 pKa = 4.38FDD254 pKa = 3.67QDD256 pKa = 3.33TDD258 pKa = 3.95DD259 pKa = 3.76VSVTFKK265 pKa = 10.86HH266 pKa = 6.76LYY268 pKa = 8.03SQSNGEE274 pKa = 3.96EE275 pKa = 4.32GHH277 pKa = 5.12WAIYY281 pKa = 10.0KK282 pKa = 10.65DD283 pKa = 3.78GVLVAQGDD291 pKa = 3.91FTEE294 pKa = 5.12DD295 pKa = 3.17VAGSGTGTIDD305 pKa = 2.73ISGYY309 pKa = 11.13GDD311 pKa = 3.79FDD313 pKa = 4.28QLVLSANLQTDD324 pKa = 3.69GTDD327 pKa = 3.24GSDD330 pKa = 4.0YY331 pKa = 10.39MVTDD335 pKa = 3.41ITFAVPPEE343 pKa = 4.21VPADD347 pKa = 3.85GDD349 pKa = 3.88DD350 pKa = 4.89SIDD353 pKa = 3.94GGDD356 pKa = 4.06GDD358 pKa = 5.27DD359 pKa = 4.24VIYY362 pKa = 10.43GQGGDD367 pKa = 3.7DD368 pKa = 4.11TISGGSGADD377 pKa = 3.48TLDD380 pKa = 3.78GGAGDD385 pKa = 4.66DD386 pKa = 4.75SIIAGEE392 pKa = 4.33GDD394 pKa = 3.96SASGGAGDD402 pKa = 5.11DD403 pKa = 3.79YY404 pKa = 10.95FTIVSDD410 pKa = 3.68EE411 pKa = 4.23AFVTGDD417 pKa = 3.09ITITGGDD424 pKa = 3.79DD425 pKa = 3.83GEE427 pKa = 4.83TTGDD431 pKa = 3.38TLDD434 pKa = 4.93FNGQLDD440 pKa = 3.72KK441 pKa = 10.89GTLVITDD448 pKa = 3.71DD449 pKa = 3.75TGGSKK454 pKa = 9.21TGSATLLDD462 pKa = 3.99GSTVYY467 pKa = 10.38FSGIEE472 pKa = 3.95NIICFTAGTRR482 pKa = 11.84IATSGGLRR490 pKa = 11.84AIEE493 pKa = 3.96TLRR496 pKa = 11.84PGDD499 pKa = 3.81LVMTRR504 pKa = 11.84DD505 pKa = 3.48NGLQPVRR512 pKa = 11.84WISQSTVPATGNLAPIRR529 pKa = 11.84IKK531 pKa = 10.68AGQFGAEE538 pKa = 3.98RR539 pKa = 11.84DD540 pKa = 3.93LLVSPQHH547 pKa = 6.73RR548 pKa = 11.84MLFSGSQASLLFGEE562 pKa = 4.8SEE564 pKa = 4.43VLVSAVHH571 pKa = 5.52MLNDD575 pKa = 3.5RR576 pKa = 11.84SIRR579 pKa = 11.84RR580 pKa = 11.84EE581 pKa = 3.57PGGTVTYY588 pKa = 9.84VHH590 pKa = 6.33VLFGQHH596 pKa = 5.92EE597 pKa = 4.44IIYY600 pKa = 10.38AEE602 pKa = 4.54GAASEE607 pKa = 4.53SFHH610 pKa = 6.97PGVQAVSALEE620 pKa = 3.93EE621 pKa = 4.12PVRR624 pKa = 11.84EE625 pKa = 4.06EE626 pKa = 4.72LFCIMPDD633 pKa = 3.71LRR635 pKa = 11.84WHH637 pKa = 5.98SGEE640 pKa = 3.92GGRR643 pKa = 11.84TARR646 pKa = 11.84LCASRR651 pKa = 11.84CEE653 pKa = 4.15AALLLPPSPLDD664 pKa = 3.28QQ665 pKa = 5.04

Molecular weight: 68.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4EHH4|A0A0B4EHH4_9RHOB Chromate transporter OS=Leisingera sp. ANG1 OX=1002340 GN=GC1_00990 PE=3 SV=1
MM1 pKa = 6.81NANWIIRR8 pKa = 11.84MVLRR12 pKa = 11.84RR13 pKa = 11.84LIGRR17 pKa = 11.84AVNAGLRR24 pKa = 11.84RR25 pKa = 11.84AAQHH29 pKa = 5.75GAQRR33 pKa = 11.84LGKK36 pKa = 10.1SKK38 pKa = 10.84GPAPGFGAGPSPQRR52 pKa = 11.84KK53 pKa = 8.27FRR55 pKa = 11.84NARR58 pKa = 11.84SRR60 pKa = 11.84IPKK63 pKa = 9.63VKK65 pKa = 10.52

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4159

0

4159

1298420

37

2149

312.2

33.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.523 ± 0.048

0.972 ± 0.013

5.599 ± 0.032

6.315 ± 0.031

3.76 ± 0.024

8.739 ± 0.038

2.087 ± 0.017

4.882 ± 0.025

3.511 ± 0.03

10.139 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.017

2.653 ± 0.018

5.025 ± 0.028

3.57 ± 0.022

6.303 ± 0.034

5.269 ± 0.024

5.148 ± 0.025

6.994 ± 0.032

1.398 ± 0.016

2.303 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski