Calothrix brevissima NIES-22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Calotrichaceae; Calothrix; Calothrix brevissima

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Z4JWQ5|A0A1Z4JWQ5_9CYAN Cell wall hydrolase/autolysin OS=Calothrix brevissima NIES-22 OX=1973478 GN=NIES22_11700 PE=4 SV=1
MM1 pKa = 7.79SYY3 pKa = 8.12PTKK6 pKa = 10.56SFSIPNPVHH15 pKa = 6.6LVFIDD20 pKa = 3.99SAIQDD25 pKa = 3.3IQTLVEE31 pKa = 4.37GVVADD36 pKa = 3.84AQVVVLHH43 pKa = 6.38PEE45 pKa = 3.71VDD47 pKa = 3.34GVMQITQTLQNQPQIGTIHH66 pKa = 6.74LVAHH70 pKa = 7.17GYY72 pKa = 8.81PGCLCLGNAQLSLKK86 pKa = 8.98TLEE89 pKa = 4.44NYY91 pKa = 10.47APEE94 pKa = 3.92LRR96 pKa = 11.84IWRR99 pKa = 11.84EE100 pKa = 3.64VMSQQGQMLLYY111 pKa = 8.96GCHH114 pKa = 4.99VAAGEE119 pKa = 4.02MGAEE123 pKa = 4.81FVQKK127 pKa = 10.17LHH129 pKa = 6.12QLTGVKK135 pKa = 9.74IAASTTAIGNAAAGGNWEE153 pKa = 4.23LDD155 pKa = 3.46VRR157 pKa = 11.84LEE159 pKa = 3.96RR160 pKa = 11.84AATQVQPEE168 pKa = 4.41LPLAFRR174 pKa = 11.84AEE176 pKa = 3.8AMAAYY181 pKa = 10.11AGVLNTAPSSLIGSLPFDD199 pKa = 3.43VLKK202 pKa = 10.94NQWKK206 pKa = 8.1TLTINDD212 pKa = 4.58LLAGIIDD219 pKa = 4.63PDD221 pKa = 3.99NDD223 pKa = 3.88PLTITNLTASAGTILKK239 pKa = 10.18RR240 pKa = 11.84IDD242 pKa = 3.21GSYY245 pKa = 10.74SYY247 pKa = 10.52IPPVDD252 pKa = 4.08FFGGITFTYY261 pKa = 10.25DD262 pKa = 2.92VSDD265 pKa = 3.92GQNPPSQQTRR275 pKa = 11.84TVEE278 pKa = 3.95VLNQRR283 pKa = 11.84FPVLDD288 pKa = 3.58EE289 pKa = 4.86FGAGAAYY296 pKa = 10.54SNQAGVSDD304 pKa = 5.12LIKK307 pKa = 10.78VYY309 pKa = 10.17WDD311 pKa = 3.33YY312 pKa = 11.06DD313 pKa = 3.79TNGNVIATSRR323 pKa = 11.84NLAAAGNTTPSVLDD337 pKa = 3.45NDD339 pKa = 3.65IPSINFTWVSGNIPSQIGVLGFNTSNGGARR369 pKa = 11.84GIMWAVLGLVGKK381 pKa = 9.91SYY383 pKa = 10.54PDD385 pKa = 3.22EE386 pKa = 4.46IYY388 pKa = 10.51RR389 pKa = 11.84VGPYY393 pKa = 9.83RR394 pKa = 11.84DD395 pKa = 3.04TATNFFTSLQMDD407 pKa = 3.81VPGGVSGFINLSNPEE422 pKa = 4.16LKK424 pKa = 10.41FALEE428 pKa = 3.73QGKK431 pKa = 9.15PITEE435 pKa = 4.19SYY437 pKa = 10.64AYY439 pKa = 10.92LVMHH443 pKa = 6.98VDD445 pKa = 3.28NTVNHH450 pKa = 6.6PLISDD455 pKa = 3.29QDD457 pKa = 3.65VRR459 pKa = 11.84NGEE462 pKa = 4.08IVYY465 pKa = 8.4ATYY468 pKa = 10.37GYY470 pKa = 10.43YY471 pKa = 10.63NYY473 pKa = 8.96VTLSLEE479 pKa = 4.19RR480 pKa = 11.84NAPPVLTPKK489 pKa = 10.45SLSFQEE495 pKa = 4.93DD496 pKa = 3.71SGFQWIDD503 pKa = 2.99SRR505 pKa = 11.84LLEE508 pKa = 4.26GLQDD512 pKa = 3.63EE513 pKa = 4.75LAPGGFSSSPEE524 pKa = 3.87TFRR527 pKa = 11.84FGDD530 pKa = 3.5WFATYY535 pKa = 10.94GLTRR539 pKa = 11.84IDD541 pKa = 5.08GIPGVQALMQSIFYY555 pKa = 9.25TLNLYY560 pKa = 10.47DD561 pKa = 5.0LGGDD565 pKa = 3.31YY566 pKa = 9.91TAYY569 pKa = 10.65VLADD573 pKa = 3.7TGHH576 pKa = 7.17AAYY579 pKa = 10.05QHH581 pKa = 6.81LAQGATQTIVGSYY594 pKa = 7.92TVQDD598 pKa = 3.97DD599 pKa = 4.67FYY601 pKa = 9.69KK602 pKa = 10.72TSTAPLTVTFTGVNDD617 pKa = 3.82APVAVADD624 pKa = 4.26TARR627 pKa = 11.84GLPNTIIEE635 pKa = 4.53GSVATNDD642 pKa = 3.38SDD644 pKa = 4.41VDD646 pKa = 3.79DD647 pKa = 4.89GAILTYY653 pKa = 10.67SLSAPVDD660 pKa = 3.76GLTLNADD667 pKa = 3.55GSYY670 pKa = 10.92SFDD673 pKa = 3.32ATRR676 pKa = 11.84PAYY679 pKa = 10.46SSLPPGQILPVVVNYY694 pKa = 10.39RR695 pKa = 11.84VTDD698 pKa = 3.53EE699 pKa = 5.11FGAFSDD705 pKa = 3.85STLTLTLTLNSLPTGSPTVTLSDD728 pKa = 3.59TAEE731 pKa = 4.17DD732 pKa = 3.55TAITINTADD741 pKa = 4.21LLAGFSDD748 pKa = 4.27VEE750 pKa = 4.22GDD752 pKa = 3.64TLSVVGLTATNGSLVNNNDD771 pKa = 2.7GTYY774 pKa = 10.42TFTPTANYY782 pKa = 9.54NGNVTLTYY790 pKa = 10.46GVSDD794 pKa = 4.81DD795 pKa = 5.41KK796 pKa = 11.18EE797 pKa = 4.28TLANQTRR804 pKa = 11.84SFSVTPVNDD813 pKa = 3.72APVGSPTATLANTPEE828 pKa = 3.99DD829 pKa = 3.23TAIIINSTGLLIGFSDD845 pKa = 4.31VEE847 pKa = 4.27GDD849 pKa = 3.64TLSVVGLTATNGSLVNNNNGTYY871 pKa = 10.16TFTPTANYY879 pKa = 9.54NGNVTLTYY887 pKa = 10.04GVSDD891 pKa = 3.58GTVTLTGQTRR901 pKa = 11.84SFSVTPVNDD910 pKa = 3.72APVGSPTATLANTRR924 pKa = 11.84EE925 pKa = 4.01DD926 pKa = 3.18TAIIINSADD935 pKa = 3.56LLAGFSDD942 pKa = 3.52VDD944 pKa = 3.8GNNTLSVVGLTATNGSLVNNNNGTYY969 pKa = 10.16TFTPTANYY977 pKa = 9.54NGNVTLTYY985 pKa = 10.04GVSDD989 pKa = 3.69GTVTLAGQTRR999 pKa = 11.84SFSVTPVNDD1008 pKa = 3.44APVAQSPPATTYY1020 pKa = 10.47QAINTPYY1027 pKa = 10.24QAIDD1031 pKa = 3.82LVPGGSGVVSILSNDD1046 pKa = 3.84DD1047 pKa = 4.17DD1048 pKa = 4.89NFAQINLGGASFNFYY1063 pKa = 11.21GNTYY1067 pKa = 10.03NSLFASSNGLITFGTGEE1084 pKa = 4.09TGFFNTDD1091 pKa = 2.98LSSGLGGLTNEE1102 pKa = 4.19IRR1104 pKa = 11.84QAAIAVFWDD1113 pKa = 3.71DD1114 pKa = 5.68LSTQGTTANQVLYY1127 pKa = 10.55RR1128 pKa = 11.84IQDD1131 pKa = 3.34NQLIVEE1137 pKa = 4.41WKK1139 pKa = 8.83EE1140 pKa = 3.59VFIRR1144 pKa = 11.84GSGSGNPITFQAILQLNTGLARR1166 pKa = 11.84GNITFNYY1173 pKa = 9.51VDD1175 pKa = 4.76LDD1177 pKa = 4.02AGNVSYY1183 pKa = 11.67NNGASATVGIKK1194 pKa = 10.28NATGSIGSPVLVSLNNGSNPNVRR1217 pKa = 11.84SGQAITIFEE1226 pKa = 4.44VGPLGYY1232 pKa = 7.51TTEE1235 pKa = 4.12DD1236 pKa = 2.92TAIIINSADD1245 pKa = 3.76LLTSFSDD1252 pKa = 3.93VEE1254 pKa = 4.3GDD1256 pKa = 3.51TLSVVNLTATNGSLVNNNNGTYY1278 pKa = 10.16TFTPTANFNGKK1289 pKa = 9.63VNLTYY1294 pKa = 10.55GVSDD1298 pKa = 5.56GIDD1301 pKa = 3.27TLSGQVQTFPVIPVNDD1317 pKa = 4.06APVGSPTATLANTRR1331 pKa = 11.84EE1332 pKa = 4.01DD1333 pKa = 3.18TAIIINSADD1342 pKa = 3.56LLAGFSDD1349 pKa = 4.27VEE1351 pKa = 4.22GDD1353 pKa = 3.64TLSVVGLTATNGSLVNNNNGTYY1375 pKa = 10.16TFTPTANFKK1384 pKa = 9.72GTVTLTYY1391 pKa = 9.86GVSDD1395 pKa = 3.69GTVTLAGQTRR1405 pKa = 11.84SFSVTPINDD1414 pKa = 3.58APVGSPTATLSDD1426 pKa = 3.95TPEE1429 pKa = 3.95DD1430 pKa = 3.22TAIIINSADD1439 pKa = 3.56LLAGFSDD1446 pKa = 4.18ANGDD1450 pKa = 3.81TLSVVGLTADD1460 pKa = 3.47NGSLVINNDD1469 pKa = 2.47GTYY1472 pKa = 10.2TFNPTANFNGAVNLTYY1488 pKa = 10.81GVTDD1492 pKa = 4.03GTATLASQTRR1502 pKa = 11.84SFNVTAINDD1511 pKa = 3.9APVGSPTATLANTSEE1526 pKa = 4.05DD1527 pKa = 3.33TAINITAADD1536 pKa = 4.13LLEE1539 pKa = 4.67GFSDD1543 pKa = 3.67VDD1545 pKa = 3.46GDD1547 pKa = 3.89NLSVVGLTADD1557 pKa = 3.47NGSLVINNDD1566 pKa = 2.47GTYY1569 pKa = 10.2TFNPTANFNGAVNLTYY1585 pKa = 10.6SVSDD1589 pKa = 3.45GTASLTGRR1597 pKa = 11.84TRR1599 pKa = 11.84TFTVTAVNDD1608 pKa = 3.96APVGSPTATLSNTAEE1623 pKa = 4.15DD1624 pKa = 3.6TAITINSSNLLEE1636 pKa = 4.71GFSDD1640 pKa = 3.57VDD1642 pKa = 3.46GDD1644 pKa = 3.89NLSVVGLTADD1654 pKa = 3.47NGSLVINNDD1663 pKa = 2.47GTYY1666 pKa = 10.2TFNPTANFNGAVNLTYY1682 pKa = 10.6SVSDD1686 pKa = 3.45GTASLTGRR1694 pKa = 11.84TRR1696 pKa = 11.84TFTVTAVNDD1705 pKa = 3.96APVGSPTATLSNTAEE1720 pKa = 4.15DD1721 pKa = 3.6TAITINSSNLLAGFSDD1737 pKa = 3.52VDD1739 pKa = 3.59GDD1741 pKa = 3.89NLSVVGLTATNGSLVNNNNGTYY1763 pKa = 10.16TFTPTANFNGTVTLTYY1779 pKa = 10.83DD1780 pKa = 3.56VTDD1783 pKa = 4.03GTATLAGQTRR1793 pKa = 11.84SFNITAVNDD1802 pKa = 3.82APTGSPTATLSNTAEE1817 pKa = 3.93DD1818 pKa = 3.6TAINITVANLLAGFSDD1834 pKa = 3.6VDD1836 pKa = 3.63GDD1838 pKa = 3.99TLSVVNLTANNGSLVNNNNGTYY1860 pKa = 10.16TFTPTANFNGTVNLTYY1876 pKa = 10.48GVSDD1880 pKa = 3.8GTATFAGQTRR1890 pKa = 11.84SFAVTPVNDD1899 pKa = 4.32APTGSPTAALSNTPEE1914 pKa = 3.92DD1915 pKa = 3.36TAINITASSLLAGFTDD1931 pKa = 3.35VDD1933 pKa = 3.95GDD1935 pKa = 3.96TLSVVNLTATNGALVNNNNGTYY1957 pKa = 10.16TFTPTANFNGTVNLTYY1973 pKa = 10.48GVSDD1977 pKa = 3.82GTATLAGQTRR1987 pKa = 11.84SFAVTPVNDD1996 pKa = 4.32APTGSPTAALSNTPEE2011 pKa = 3.77EE2012 pKa = 3.97TAINITASSLLAGFTDD2028 pKa = 3.35VDD2030 pKa = 3.95GDD2032 pKa = 3.96TLSVVNLTATNGALVNNNNGTYY2054 pKa = 10.19TFTPNTNFNGTVNLTYY2070 pKa = 10.3GVSDD2074 pKa = 3.59GTVTLAGQTRR2084 pKa = 11.84SFTVTPVNDD2093 pKa = 3.83APVGNPDD2100 pKa = 3.48TVSTTRR2106 pKa = 11.84NTAVNIAVSTLLANDD2121 pKa = 3.76TDD2123 pKa = 3.63IDD2125 pKa = 4.03TPLASLRR2132 pKa = 11.84ITAVSGATNGTVSLNNKK2149 pKa = 7.01GTPTNFSDD2157 pKa = 5.26DD2158 pKa = 4.54FITFTPQNNFTGNATFNYY2176 pKa = 8.06TISDD2180 pKa = 3.69GSLTSTATVAVSVGLSLNGSNGSNILTGSDD2210 pKa = 3.68FSDD2213 pKa = 5.16LINGNDD2219 pKa = 4.27GDD2221 pKa = 5.49DD2222 pKa = 3.27ILYY2225 pKa = 10.78GNNGNDD2231 pKa = 3.47TLIGGNGLDD2240 pKa = 4.08LLLGGEE2246 pKa = 4.96GNDD2249 pKa = 3.78SLNGGGGADD2258 pKa = 3.36ALYY2261 pKa = 10.54GGKK2264 pKa = 10.8GNDD2267 pKa = 3.47TLTGGSGIDD2276 pKa = 3.05IFAFAQGDD2284 pKa = 4.02GSDD2287 pKa = 4.12TITDD2291 pKa = 3.91FTDD2294 pKa = 5.05GIDD2297 pKa = 3.57WIGLVGLRR2305 pKa = 11.84FNQLSISGNGGNTTIRR2321 pKa = 11.84YY2322 pKa = 9.55GNEE2325 pKa = 3.3TLATLTGVSANLITAADD2342 pKa = 5.11FISVV2346 pKa = 3.3

Molecular weight: 242.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Z4JYW6|A0A1Z4JYW6_9CYAN CMP/dCMP deaminase zinc-binding protein OS=Calothrix brevissima NIES-22 OX=1973478 GN=NIES22_19000 PE=4 SV=1
MM1 pKa = 7.08QRR3 pKa = 11.84TLGGTNRR10 pKa = 11.84KK11 pKa = 9.1RR12 pKa = 11.84KK13 pKa = 7.65RR14 pKa = 11.84TSGFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TPDD26 pKa = 3.17GRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84KK36 pKa = 9.01KK37 pKa = 9.6GRR39 pKa = 11.84HH40 pKa = 5.0RR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7229

0

7229

2460895

29

10429

340.4

37.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.191 ± 0.033

0.931 ± 0.011

4.88 ± 0.028

6.038 ± 0.03

3.896 ± 0.018

6.518 ± 0.04

1.771 ± 0.014

7.076 ± 0.026

4.993 ± 0.03

10.886 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.696 ± 0.017

4.868 ± 0.039

4.6 ± 0.024

5.658 ± 0.031

4.818 ± 0.027

6.467 ± 0.024

5.79 ± 0.034

6.398 ± 0.023

1.394 ± 0.014

3.13 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski