Lishi spider virus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Jingchuvirales; Chuviridae; Scarabeuvirus; Scarabeuvirus lishiense

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B5KJV2|A0A0B5KJV2_9VIRU Putative nucleoprotein OS=Lishi spider virus 1 OX=1608057 GN=N PE=4 SV=1
MM1 pKa = 7.73LILEE5 pKa = 4.58SNMVNYY11 pKa = 9.2MIKK14 pKa = 10.59LLFLPTCYY22 pKa = 10.59GFLAFDD28 pKa = 4.41CSNPNINYY36 pKa = 8.29TLVNTRR42 pKa = 11.84EE43 pKa = 3.98VEE45 pKa = 3.98EE46 pKa = 5.25CIISTPEE53 pKa = 3.44IVEE56 pKa = 4.21EE57 pKa = 4.11KK58 pKa = 11.07VSIQLLQLNSIAYY71 pKa = 9.1AKK73 pKa = 10.42YY74 pKa = 9.03VSCKK78 pKa = 9.65VSMNRR83 pKa = 11.84IISYY87 pKa = 10.55CGMNSHH93 pKa = 6.34VSMVHH98 pKa = 5.58NGYY101 pKa = 11.1ASFIVPITNTEE112 pKa = 3.75CWNIHH117 pKa = 5.22RR118 pKa = 11.84DD119 pKa = 3.34RR120 pKa = 11.84RR121 pKa = 11.84YY122 pKa = 9.72ILPNLSVVSNLKK134 pKa = 10.02PNSTIEE140 pKa = 4.04VSSVLSGKK148 pKa = 10.02LFSDD152 pKa = 4.94GKK154 pKa = 11.04CEE156 pKa = 4.04GGSYY160 pKa = 9.4NDD162 pKa = 5.25KK163 pKa = 9.92YY164 pKa = 11.3GSWEE168 pKa = 4.11SVIVQAIYY176 pKa = 10.35KK177 pKa = 9.27IQLTEE182 pKa = 4.61GYY184 pKa = 7.35TQYY187 pKa = 11.55DD188 pKa = 3.38RR189 pKa = 11.84DD190 pKa = 3.68TNYY193 pKa = 9.75IHH195 pKa = 7.21LPYY198 pKa = 10.27GINCIYY204 pKa = 10.95DD205 pKa = 4.3RR206 pKa = 11.84IDD208 pKa = 3.79CVHH211 pKa = 7.42PDD213 pKa = 2.9IGMTTWDD220 pKa = 3.46VFPTHH225 pKa = 6.55EE226 pKa = 4.79CDD228 pKa = 4.28DD229 pKa = 3.8NRR231 pKa = 11.84HH232 pKa = 5.64LVLFDD237 pKa = 3.65GFASKK242 pKa = 7.73TTTQSLTGEE251 pKa = 3.99NMKK254 pKa = 10.33VVTYY258 pKa = 9.0MVTSSTRR265 pKa = 11.84AFALSVTTRR274 pKa = 11.84YY275 pKa = 9.63DD276 pKa = 3.22KK277 pKa = 11.34CSMEE281 pKa = 4.3VWRR284 pKa = 11.84TEE286 pKa = 3.86HH287 pKa = 6.71PRR289 pKa = 11.84LLILVVNQQWDD300 pKa = 4.03KK301 pKa = 11.13IKK303 pKa = 11.11SNSIDD308 pKa = 3.34ISSLDD313 pKa = 3.29LMAYY317 pKa = 10.19VNAKK321 pKa = 10.37LIMIQASVASNVEE334 pKa = 4.03SLYY337 pKa = 9.36QTLDD341 pKa = 3.01YY342 pKa = 9.79RR343 pKa = 11.84RR344 pKa = 11.84CMAEE348 pKa = 3.86RR349 pKa = 11.84KK350 pKa = 9.46SLRR353 pKa = 11.84NQLALASLSPQEE365 pKa = 3.87FAYY368 pKa = 10.65LYY370 pKa = 8.9MGKK373 pKa = 9.26PGYY376 pKa = 9.04TSLPMGEE383 pKa = 3.9VSYY386 pKa = 10.52IIQCNPVEE394 pKa = 4.34VKK396 pKa = 10.24LRR398 pKa = 11.84KK399 pKa = 8.07TDD401 pKa = 3.05KK402 pKa = 10.43CYY404 pKa = 10.82HH405 pKa = 6.38EE406 pKa = 5.62LPISLNNQSFYY417 pKa = 10.58MSPKK421 pKa = 8.62TRR423 pKa = 11.84LIQSQGTEE431 pKa = 4.18VEE433 pKa = 4.86CNHH436 pKa = 6.61LMLPKK441 pKa = 10.33FLLGKK446 pKa = 9.57NWYY449 pKa = 9.18EE450 pKa = 3.91IYY452 pKa = 10.8NGARR456 pKa = 11.84LSEE459 pKa = 4.27VPTTLSANYY468 pKa = 8.57EE469 pKa = 4.28HH470 pKa = 6.77GWKK473 pKa = 8.29YY474 pKa = 9.73TSIKK478 pKa = 10.52DD479 pKa = 3.63LASSGIYY486 pKa = 10.09SQDD489 pKa = 4.34DD490 pKa = 3.09IDD492 pKa = 3.68KK493 pKa = 10.07LRR495 pKa = 11.84RR496 pKa = 11.84YY497 pKa = 9.94IMAPNEE503 pKa = 3.7RR504 pKa = 11.84TAITNTLSDD513 pKa = 4.17VINGNIEE520 pKa = 4.04LSPDD524 pKa = 3.52LKK526 pKa = 9.93VTNLIGPQTLEE537 pKa = 3.94SLASKK542 pKa = 10.25LSSKK546 pKa = 9.11IWSMFTLFGHH556 pKa = 6.2VSSGVIGIYY565 pKa = 9.62MLARR569 pKa = 11.84LIKK572 pKa = 10.46LIVDD576 pKa = 4.2TAVHH580 pKa = 5.78GTILYY585 pKa = 10.05NLYY588 pKa = 9.99GWSICLIGGIWDD600 pKa = 4.39SITHH604 pKa = 4.87YY605 pKa = 10.65LIYY608 pKa = 10.33RR609 pKa = 11.84KK610 pKa = 9.39RR611 pKa = 11.84HH612 pKa = 4.23EE613 pKa = 4.41TKK615 pKa = 10.67KK616 pKa = 10.95GDD618 pKa = 3.41TTMEE622 pKa = 4.19TEE624 pKa = 4.17SLEE627 pKa = 4.37VSAKK631 pKa = 10.59DD632 pKa = 4.04YY633 pKa = 11.02ISEE636 pKa = 4.27APLKK640 pKa = 10.31NIHH643 pKa = 6.68INQQRR648 pKa = 11.84VTYY651 pKa = 9.31PNLEE655 pKa = 3.76RR656 pKa = 11.84FMPP659 pKa = 4.64

Molecular weight: 75.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B5KRA0|A0A0B5KRA0_9VIRU Large structural protein OS=Lishi spider virus 1 OX=1608057 GN=L PE=4 SV=1
MM1 pKa = 7.61ANDD4 pKa = 3.11NVMRR8 pKa = 11.84LLGRR12 pKa = 11.84ALNEE16 pKa = 3.91PPIDD20 pKa = 3.75EE21 pKa = 4.47PVGLRR26 pKa = 11.84GPCMNTAFFSKK37 pKa = 9.9WLRR40 pKa = 11.84QDD42 pKa = 3.93IIFQKK47 pKa = 10.65VGVRR51 pKa = 11.84SIDD54 pKa = 3.38VAPFQGDD61 pKa = 3.13FDD63 pKa = 6.0DD64 pKa = 5.06NLVPTMIHH72 pKa = 6.96LYY74 pKa = 10.56SIGKK78 pKa = 8.46FLHH81 pKa = 6.22YY82 pKa = 10.03KK83 pKa = 8.7PQSTVSRR90 pKa = 11.84AAVLAITCAEE100 pKa = 4.09TLGPSIGTDD109 pKa = 2.55RR110 pKa = 11.84LMTQHH115 pKa = 7.05EE116 pKa = 4.38ARR118 pKa = 11.84QSLCHH123 pKa = 6.04AVCALRR129 pKa = 11.84NSGLLATFTSATAQTYY145 pKa = 9.15GALVGNDD152 pKa = 4.47RR153 pKa = 11.84PAQWTDD159 pKa = 3.15TDD161 pKa = 3.54WDD163 pKa = 4.03STDD166 pKa = 3.49VEE168 pKa = 4.67ANCRR172 pKa = 11.84VLRR175 pKa = 11.84HH176 pKa = 4.93MALRR180 pKa = 11.84AEE182 pKa = 4.32AAYY185 pKa = 8.54TLKK188 pKa = 10.67GSSLIVHH195 pKa = 6.5MLVAIIKK202 pKa = 9.78RR203 pKa = 11.84GTMSDD208 pKa = 3.22AFVDD212 pKa = 4.93KK213 pKa = 10.23IQQGIQTDD221 pKa = 4.16LSLPIRR227 pKa = 11.84ISAEE231 pKa = 3.76TCRR234 pKa = 11.84IFYY237 pKa = 10.58SSYY240 pKa = 10.36CSEE243 pKa = 4.31LNDD246 pKa = 3.37VGMSFLLSHH255 pKa = 7.75LDD257 pKa = 3.5RR258 pKa = 11.84LIPTHH263 pKa = 6.65ALRR266 pKa = 11.84LRR268 pKa = 11.84LTIQQASGSGLTALVLLGRR287 pKa = 11.84AFRR290 pKa = 11.84MYY292 pKa = 11.23EE293 pKa = 3.83NFNWPLVAKK302 pKa = 8.9WYY304 pKa = 9.05PDD306 pKa = 2.64EE307 pKa = 4.2WANFTAAVDD316 pKa = 4.0SVGNNLMYY324 pKa = 10.53GYY326 pKa = 10.36RR327 pKa = 11.84RR328 pKa = 11.84DD329 pKa = 3.35IGPAKK334 pKa = 8.97STKK337 pKa = 9.29FRR339 pKa = 11.84SLTFIAKK346 pKa = 9.62EE347 pKa = 3.46LLIRR351 pKa = 11.84VGGEE355 pKa = 3.68TSLRR359 pKa = 11.84NYY361 pKa = 10.61AGFATRR367 pKa = 11.84VKK369 pKa = 10.82NHH371 pKa = 6.98DD372 pKa = 3.52AVINLINTYY381 pKa = 8.24VDD383 pKa = 4.67RR384 pKa = 11.84ITAAQAEE391 pKa = 4.8VEE393 pKa = 4.35VGDD396 pKa = 4.49AEE398 pKa = 4.55SEE400 pKa = 4.13AVNQFRR406 pKa = 11.84QTVVEE411 pKa = 4.04HH412 pKa = 6.42ANVYY416 pKa = 9.62GG417 pKa = 3.59

Molecular weight: 46.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

3256

417

2180

1085.3

124.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.129 ± 1.14

2.119 ± 0.113

4.668 ± 0.238

6.081 ± 0.635

4.146 ± 0.687

4.361 ± 0.535

2.887 ± 0.249

7.033 ± 0.47

5.006 ± 0.483

10.565 ± 0.331

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.328

5.283 ± 0.302

4.115 ± 0.309

3.194 ± 0.141

5.59 ± 0.481

8.016 ± 0.504

6.91 ± 0.026

6.081 ± 0.339

1.413 ± 0.041

4.914 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski