Gordonia phage Ziko

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ronaldovirus; unclassified Ronaldovirus

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 149 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A514A556|A0A514A556_9CAUD Uncharacterized protein OS=Gordonia phage Ziko OX=2591193 GN=69 PE=4 SV=1
MM1 pKa = 7.51AVTAKK6 pKa = 10.6VFGPFLNSMADD17 pKa = 3.33KK18 pKa = 10.94LVDD21 pKa = 4.49LNSDD25 pKa = 4.05TIKK28 pKa = 11.2VMLTTSAPDD37 pKa = 3.05QDD39 pKa = 3.12AWQFKK44 pKa = 10.31SSVTGEE50 pKa = 4.23VTGTGYY56 pKa = 8.29TAGGATVASPTFSYY70 pKa = 9.04NTSTNVWMFDD80 pKa = 3.32GADD83 pKa = 3.83VTWSSSTITANYY95 pKa = 9.87AVFYY99 pKa = 10.24DD100 pKa = 3.94STPATDD106 pKa = 3.21ATRR109 pKa = 11.84PLICYY114 pKa = 8.78WDD116 pKa = 3.65FGGAQSSSSGNFTLSFSASGLVTATVAA143 pKa = 3.78

Molecular weight: 14.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A514A5C4|A0A514A5C4_9CAUD TRASH domain-containing protein OS=Gordonia phage Ziko OX=2591193 GN=138 PE=4 SV=1
MM1 pKa = 7.44NGLSVFGSSPTVATTRR17 pKa = 11.84PCSPIGRR24 pKa = 11.84GTRR27 pKa = 11.84SRR29 pKa = 11.84DD30 pKa = 3.09EE31 pKa = 4.25TIAANLITPNPTRR44 pKa = 11.84QIDD47 pKa = 3.65CRR49 pKa = 11.84AYY51 pKa = 8.35TLNNAGSNPAPGTKK65 pKa = 10.27AFVLL69 pKa = 4.13

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

149

0

149

21086

37

1851

141.5

15.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.731 ± 0.542

1.513 ± 0.194

6.72 ± 0.238

7.218 ± 0.369

3.31 ± 0.121

7.953 ± 0.511

1.978 ± 0.143

5.136 ± 0.139

4.828 ± 0.268

7.811 ± 0.238

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.812 ± 0.146

4.098 ± 0.198

4.382 ± 0.194

3.604 ± 0.157

5.511 ± 0.211

6.379 ± 0.255

5.791 ± 0.248

6.739 ± 0.244

2.087 ± 0.164

3.4 ± 0.159
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski