Amino acid dipepetide frequency for Bombyx mori (Silk moth)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.535AlaAla: 7.535 ± 0.081
1.426AlaCys: 1.426 ± 0.039
3.549AlaAsp: 3.549 ± 0.028
4.373AlaGlu: 4.373 ± 0.039
2.316AlaPhe: 2.316 ± 0.025
4.596AlaGly: 4.596 ± 0.269
1.824AlaHis: 1.824 ± 0.024
3.397AlaIle: 3.397 ± 0.029
3.635AlaLys: 3.635 ± 0.029
7.152AlaLeu: 7.152 ± 0.065
1.514AlaMet: 1.514 ± 0.017
2.626AlaAsn: 2.626 ± 0.024
4.173AlaPro: 4.173 ± 0.046
2.598AlaGln: 2.598 ± 0.026
4.224AlaArg: 4.224 ± 0.044
5.011AlaSer: 5.011 ± 0.039
3.867AlaThr: 3.867 ± 0.032
4.868AlaVal: 4.868 ± 0.038
0.761AlaTrp: 0.761 ± 0.012
1.909AlaTyr: 1.909 ± 0.026
0.002AlaXaa: 0.002 ± 0.001
Cys
1.416CysAla: 1.416 ± 0.024
0.476CysCys: 0.476 ± 0.012
1.191CysAsp: 1.191 ± 0.022
1.246CysGlu: 1.246 ± 0.025
0.698CysPhe: 0.698 ± 0.014
1.545CysGly: 1.545 ± 0.046
0.487CysHis: 0.487 ± 0.012
1.005CysIle: 1.005 ± 0.039
1.092CysLys: 1.092 ± 0.03
1.752CysLeu: 1.752 ± 0.033
0.379CysMet: 0.379 ± 0.009
0.853CysAsn: 0.853 ± 0.02
1.061CysPro: 1.061 ± 0.038
0.673CysGln: 0.673 ± 0.023
1.179CysArg: 1.179 ± 0.037
1.534CysSer: 1.534 ± 0.041
1.109CysThr: 1.109 ± 0.028
1.391CysVal: 1.391 ± 0.034
0.226CysTrp: 0.226 ± 0.006
0.596CysTyr: 0.596 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
3.63AspAla: 3.63 ± 0.031
1.049AspCys: 1.049 ± 0.024
3.964AspAsp: 3.964 ± 0.039
4.404AspGlu: 4.404 ± 0.035
2.149AspPhe: 2.149 ± 0.022
3.277AspGly: 3.277 ± 0.031
1.199AspHis: 1.199 ± 0.015
3.345AspIle: 3.345 ± 0.027
3.482AspLys: 3.482 ± 0.035
4.94AspLeu: 4.94 ± 0.035
1.243AspMet: 1.243 ± 0.015
2.72AspAsn: 2.72 ± 0.026
2.673AspPro: 2.673 ± 0.04
1.644AspGln: 1.644 ± 0.018
2.787AspArg: 2.787 ± 0.034
4.289AspSer: 4.289 ± 0.037
2.972AspThr: 2.972 ± 0.025
3.918AspVal: 3.918 ± 0.029
0.653AspTrp: 0.653 ± 0.012
1.929AspTyr: 1.929 ± 0.018
0.002AspXaa: 0.002 ± 0.0
Glu
4.431GluAla: 4.431 ± 0.038
1.297GluCys: 1.297 ± 0.055
4.078GluAsp: 4.078 ± 0.036
5.733GluGlu: 5.733 ± 0.061
2.131GluPhe: 2.131 ± 0.024
3.181GluGly: 3.181 ± 0.028
1.566GluHis: 1.566 ± 0.022
3.972GluIle: 3.972 ± 0.038
5.025GluLys: 5.025 ± 0.057
6.036GluLeu: 6.036 ± 0.049
1.58GluMet: 1.58 ± 0.018
3.691GluAsn: 3.691 ± 0.037
2.997GluPro: 2.997 ± 0.035
2.605GluGln: 2.605 ± 0.029
3.991GluArg: 3.991 ± 0.038
4.36GluSer: 4.36 ± 0.037
3.83GluThr: 3.83 ± 0.038
3.904GluVal: 3.904 ± 0.03
0.715GluTrp: 0.715 ± 0.013
2.076GluTyr: 2.076 ± 0.022
0.001GluXaa: 0.001 ± 0.0
Phe
2.174PheAla: 2.174 ± 0.026
0.695PheCys: 0.695 ± 0.01
2.077PheAsp: 2.077 ± 0.021
2.19PheGlu: 2.19 ± 0.021
1.365PhePhe: 1.365 ± 0.02
2.33PheGly: 2.33 ± 0.032
0.872PheHis: 0.872 ± 0.012
2.005PheIle: 2.005 ± 0.021
2.156PheLys: 2.156 ± 0.022
3.164PheLeu: 3.164 ± 0.029
0.851PheMet: 0.851 ± 0.014
1.773PheAsn: 1.773 ± 0.02
1.579PhePro: 1.579 ± 0.017
1.259PheGln: 1.259 ± 0.013
1.847PheArg: 1.847 ± 0.017
2.694PheSer: 2.694 ± 0.026
2.047PheThr: 2.047 ± 0.023
2.434PheVal: 2.434 ± 0.024
0.407PheTrp: 0.407 ± 0.009
1.308PheTyr: 1.308 ± 0.015
0.001PheXaa: 0.001 ± 0.0
Gly
4.63GlyAla: 4.63 ± 0.279
1.084GlyCys: 1.084 ± 0.022
3.19GlyAsp: 3.19 ± 0.035
3.279GlyGlu: 3.279 ± 0.031
2.173GlyPhe: 2.173 ± 0.024
4.666GlyGly: 4.666 ± 0.065
1.507GlyHis: 1.507 ± 0.023
2.864GlyIle: 2.864 ± 0.025
3.211GlyLys: 3.211 ± 0.031
4.689GlyLeu: 4.689 ± 0.042
1.152GlyMet: 1.152 ± 0.017
2.455GlyAsn: 2.455 ± 0.026
2.654GlyPro: 2.654 ± 0.046
2.014GlyGln: 2.014 ± 0.044
3.269GlyArg: 3.269 ± 0.029
4.475GlySer: 4.475 ± 0.102
3.182GlyThr: 3.182 ± 0.028
3.791GlyVal: 3.791 ± 0.035
0.743GlyTrp: 0.743 ± 0.013
2.093GlyTyr: 2.093 ± 0.055
0.002GlyXaa: 0.002 ± 0.0
His
1.747HisAla: 1.747 ± 0.023
0.58HisCys: 0.58 ± 0.012
1.251HisAsp: 1.251 ± 0.016
1.448HisGlu: 1.448 ± 0.018
0.965HisPhe: 0.965 ± 0.014
1.443HisGly: 1.443 ± 0.023
0.962HisHis: 0.962 ± 0.023
1.326HisIle: 1.326 ± 0.016
1.419HisLys: 1.419 ± 0.018
2.348HisLeu: 2.348 ± 0.025
0.634HisMet: 0.634 ± 0.014
1.164HisAsn: 1.164 ± 0.015
1.344HisPro: 1.344 ± 0.019
0.926HisGln: 0.926 ± 0.014
1.519HisArg: 1.519 ± 0.02
1.907HisSer: 1.907 ± 0.023
1.396HisThr: 1.396 ± 0.016
1.655HisVal: 1.655 ± 0.018
0.327HisTrp: 0.327 ± 0.008
0.935HisTyr: 0.935 ± 0.013
0.001HisXaa: 0.001 ± 0.0
Ile
3.464IleAla: 3.464 ± 0.029
1.126IleCys: 1.126 ± 0.029
3.164IleAsp: 3.164 ± 0.029
3.726IleGlu: 3.726 ± 0.039
1.991IlePhe: 1.991 ± 0.025
2.736IleGly: 2.736 ± 0.026
1.259IleHis: 1.259 ± 0.015
3.096IleIle: 3.096 ± 0.033
3.653IleLys: 3.653 ± 0.033
4.719IleLeu: 4.719 ± 0.035
1.18IleMet: 1.18 ± 0.013
2.757IleAsn: 2.757 ± 0.031
2.762IlePro: 2.762 ± 0.024
2.091IleGln: 2.091 ± 0.021
2.63IleArg: 2.63 ± 0.023
3.981IleSer: 3.981 ± 0.024
3.164IleThr: 3.164 ± 0.028
3.549IleVal: 3.549 ± 0.029
0.527IleTrp: 0.527 ± 0.011
1.706IleTyr: 1.706 ± 0.023
0.002IleXaa: 0.002 ± 0.001
Lys
3.589LysAla: 3.589 ± 0.034
1.197LysCys: 1.197 ± 0.031
3.491LysAsp: 3.491 ± 0.032
4.68LysGlu: 4.68 ± 0.047
2.105LysPhe: 2.105 ± 0.022
2.733LysGly: 2.733 ± 0.035
1.594LysHis: 1.594 ± 0.019
3.79LysIle: 3.79 ± 0.03
5.348LysLys: 5.348 ± 0.055
5.709LysLeu: 5.709 ± 0.041
1.534LysMet: 1.534 ± 0.02
3.28LysAsn: 3.28 ± 0.032
3.212LysPro: 3.212 ± 0.039
2.549LysGln: 2.549 ± 0.024
3.727LysArg: 3.727 ± 0.029
4.525LysSer: 4.525 ± 0.039
3.71LysThr: 3.71 ± 0.033
3.602LysVal: 3.602 ± 0.029
0.645LysTrp: 0.645 ± 0.012
2.251LysTyr: 2.251 ± 0.024
0.002LysXaa: 0.002 ± 0.001
Leu
6.708LeuAla: 6.708 ± 0.048
1.877LeuCys: 1.877 ± 0.025
4.943LeuAsp: 4.943 ± 0.04
5.953LeuGlu: 5.953 ± 0.054
3.062LeuPhe: 3.062 ± 0.03
4.682LeuGly: 4.682 ± 0.036
2.44LeuHis: 2.44 ± 0.026
4.325LeuIle: 4.325 ± 0.037
6.068LeuLys: 6.068 ± 0.049
8.637LeuLeu: 8.637 ± 0.064
1.979LeuMet: 1.979 ± 0.023
4.221LeuAsn: 4.221 ± 0.031
4.762LeuPro: 4.762 ± 0.037
4.2LeuGln: 4.2 ± 0.046
5.584LeuArg: 5.584 ± 0.041
6.786LeuSer: 6.786 ± 0.048
5.05LeuThr: 5.05 ± 0.034
5.514LeuVal: 5.514 ± 0.045
0.964LeuTrp: 0.964 ± 0.016
2.755LeuTyr: 2.755 ± 0.026
0.003LeuXaa: 0.003 ± 0.001
Met
1.657MetAla: 1.657 ± 0.02
0.448MetCys: 0.448 ± 0.012
1.193MetAsp: 1.193 ± 0.015
1.493MetGlu: 1.493 ± 0.017
0.907MetPhe: 0.907 ± 0.014
1.123MetGly: 1.123 ± 0.019
0.489MetHis: 0.489 ± 0.009
1.074MetIle: 1.074 ± 0.015
1.53MetLys: 1.53 ± 0.02
2.0MetLeu: 2.0 ± 0.019
0.612MetMet: 0.612 ± 0.012
1.045MetAsn: 1.045 ± 0.015
1.127MetPro: 1.127 ± 0.017
0.884MetGln: 0.884 ± 0.015
1.217MetArg: 1.217 ± 0.018
1.813MetSer: 1.813 ± 0.021
1.225MetThr: 1.225 ± 0.016
1.287MetVal: 1.287 ± 0.017
0.26MetTrp: 0.26 ± 0.007
0.713MetTyr: 0.713 ± 0.013
0.001MetXaa: 0.001 ± 0.0
Asn
2.815AsnAla: 2.815 ± 0.031
0.851AsnCys: 0.851 ± 0.017
2.651AsnAsp: 2.651 ± 0.024
3.274AsnGlu: 3.274 ± 0.03
1.8AsnPhe: 1.8 ± 0.019
2.764AsnGly: 2.764 ± 0.032
1.03AsnHis: 1.03 ± 0.02
3.163AsnIle: 3.163 ± 0.03
3.283AsnLys: 3.283 ± 0.031
4.178AsnLeu: 4.178 ± 0.037
1.169AsnMet: 1.169 ± 0.015
2.877AsnAsn: 2.877 ± 0.037
2.207AsnPro: 2.207 ± 0.038
1.729AsnGln: 1.729 ± 0.028
2.239AsnArg: 2.239 ± 0.025
3.535AsnSer: 3.535 ± 0.032
2.747AsnThr: 2.747 ± 0.026
3.291AsnVal: 3.291 ± 0.026
0.481AsnTrp: 0.481 ± 0.01
1.677AsnTyr: 1.677 ± 0.019
0.002AsnXaa: 0.002 ± 0.001
Pro
4.198ProAla: 4.198 ± 0.047
0.865ProCys: 0.865 ± 0.058
2.91ProAsp: 2.91 ± 0.026
3.675ProGlu: 3.675 ± 0.034
1.676ProPhe: 1.676 ± 0.02
3.04ProGly: 3.04 ± 0.07
1.429ProHis: 1.429 ± 0.022
2.538ProIle: 2.538 ± 0.027
2.993ProLys: 2.993 ± 0.03
4.355ProLeu: 4.355 ± 0.034
0.964ProMet: 0.964 ± 0.016
2.262ProAsn: 2.262 ± 0.031
4.898ProPro: 4.898 ± 0.073
2.173ProGln: 2.173 ± 0.031
2.985ProArg: 2.985 ± 0.035
4.115ProSer: 4.115 ± 0.046
3.145ProThr: 3.145 ± 0.037
3.563ProVal: 3.563 ± 0.036
0.507ProTrp: 0.507 ± 0.01
1.675ProTyr: 1.675 ± 0.025
0.003ProXaa: 0.003 ± 0.001
Gln
2.536GlnAla: 2.536 ± 0.026
0.81GlnCys: 0.81 ± 0.023
1.82GlnAsp: 1.82 ± 0.02
2.531GlnGlu: 2.531 ± 0.028
1.316GlnPhe: 1.316 ± 0.015
1.765GlnGly: 1.765 ± 0.025
1.156GlnHis: 1.156 ± 0.018
2.132GlnIle: 2.132 ± 0.022
2.38GlnLys: 2.38 ± 0.025
3.751GlnLeu: 3.751 ± 0.037
0.95GlnMet: 0.95 ± 0.015
2.103GlnAsn: 2.103 ± 0.025
2.125GlnPro: 2.125 ± 0.041
2.186GlnGln: 2.186 ± 0.04
2.409GlnArg: 2.409 ± 0.028
2.61GlnSer: 2.61 ± 0.026
2.105GlnThr: 2.105 ± 0.023
2.167GlnVal: 2.167 ± 0.023
0.458GlnTrp: 0.458 ± 0.009
1.363GlnTyr: 1.363 ± 0.018
0.001GlnXaa: 0.001 ± 0.0
Arg
4.339ArgAla: 4.339 ± 0.049
1.142ArgCys: 1.142 ± 0.022
3.207ArgAsp: 3.207 ± 0.03
3.614ArgGlu: 3.614 ± 0.035
1.831ArgPhe: 1.831 ± 0.019
3.223ArgGly: 3.223 ± 0.035
1.66ArgHis: 1.66 ± 0.02
2.796ArgIle: 2.796 ± 0.025
3.582ArgLys: 3.582 ± 0.031
5.307ArgLeu: 5.307 ± 0.045
1.203ArgMet: 1.203 ± 0.016
2.596ArgAsn: 2.596 ± 0.023
3.039ArgPro: 3.039 ± 0.035
2.223ArgGln: 2.223 ± 0.024
4.606ArgArg: 4.606 ± 0.049
4.075ArgSer: 4.075 ± 0.041
3.021ArgThr: 3.021 ± 0.029
3.404ArgVal: 3.404 ± 0.03
0.661ArgTrp: 0.661 ± 0.012
1.78ArgTyr: 1.78 ± 0.022
0.002ArgXaa: 0.002 ± 0.001
Ser
5.075SerAla: 5.075 ± 0.038
1.411SerCys: 1.411 ± 0.045
4.415SerAsp: 4.415 ± 0.039
4.853SerGlu: 4.853 ± 0.037
2.542SerPhe: 2.542 ± 0.023
4.717SerGly: 4.717 ± 0.105
1.725SerHis: 1.725 ± 0.019
3.702SerIle: 3.702 ± 0.031
4.427SerLys: 4.427 ± 0.04
6.579SerLeu: 6.579 ± 0.045
1.525SerMet: 1.525 ± 0.019
3.538SerAsn: 3.538 ± 0.036
4.373SerPro: 4.373 ± 0.053
2.776SerGln: 2.776 ± 0.029
4.05SerArg: 4.05 ± 0.036
7.069SerSer: 7.069 ± 0.069
4.681SerThr: 4.681 ± 0.054
4.781SerVal: 4.781 ± 0.031
0.837SerTrp: 0.837 ± 0.014
2.196SerTyr: 2.196 ± 0.023
0.003SerXaa: 0.003 ± 0.001
Thr
4.062ThrAla: 4.062 ± 0.03
1.131ThrCys: 1.131 ± 0.026
3.129ThrAsp: 3.129 ± 0.029
3.865ThrGlu: 3.865 ± 0.049
2.062ThrPhe: 2.062 ± 0.021
3.26ThrGly: 3.26 ± 0.033
1.383ThrHis: 1.383 ± 0.017
3.074ThrIle: 3.074 ± 0.026
3.475ThrLys: 3.475 ± 0.032
5.223ThrLeu: 5.223 ± 0.037
1.156ThrMet: 1.156 ± 0.016
2.68ThrAsn: 2.68 ± 0.024
3.541ThrPro: 3.541 ± 0.041
2.047ThrGln: 2.047 ± 0.023
2.824ThrArg: 2.824 ± 0.025
4.519ThrSer: 4.519 ± 0.042
3.906ThrThr: 3.906 ± 0.099
4.013ThrVal: 4.013 ± 0.033
0.619ThrTrp: 0.619 ± 0.011
1.683ThrTyr: 1.683 ± 0.022
0.002ThrXaa: 0.002 ± 0.0
Val
4.708ValAla: 4.708 ± 0.039
1.457ValCys: 1.457 ± 0.029
3.522ValAsp: 3.522 ± 0.03
4.135ValGlu: 4.135 ± 0.036
2.378ValPhe: 2.378 ± 0.024
3.305ValGly: 3.305 ± 0.031
1.6ValHis: 1.6 ± 0.02
3.39ValIle: 3.39 ± 0.029
3.845ValLys: 3.845 ± 0.028
5.986ValLeu: 5.986 ± 0.045
1.423ValMet: 1.423 ± 0.017
2.918ValAsn: 2.918 ± 0.024
3.493ValPro: 3.493 ± 0.033
2.45ValGln: 2.45 ± 0.024
3.606ValArg: 3.606 ± 0.034
4.762ValSer: 4.762 ± 0.033
4.036ValThr: 4.036 ± 0.033
4.505ValVal: 4.505 ± 0.038
0.764ValTrp: 0.764 ± 0.014
2.022ValTyr: 2.022 ± 0.02
0.002ValXaa: 0.002 ± 0.001
Trp
0.686TrpAla: 0.686 ± 0.013
0.248TrpCys: 0.248 ± 0.007
0.636TrpAsp: 0.636 ± 0.012
0.648TrpGlu: 0.648 ± 0.01
0.435TrpPhe: 0.435 ± 0.01
0.661TrpGly: 0.661 ± 0.013
0.265TrpHis: 0.265 ± 0.008
0.552TrpIle: 0.552 ± 0.01
0.687TrpLys: 0.687 ± 0.012
1.122TrpLeu: 1.122 ± 0.018
0.291TrpMet: 0.291 ± 0.007
0.535TrpAsn: 0.535 ± 0.011
0.481TrpPro: 0.481 ± 0.011
0.434TrpGln: 0.434 ± 0.01
0.787TrpArg: 0.787 ± 0.014
0.85TrpSer: 0.85 ± 0.015
0.618TrpThr: 0.618 ± 0.012
0.639TrpVal: 0.639 ± 0.011
0.203TrpTrp: 0.203 ± 0.007
0.373TrpTyr: 0.373 ± 0.009
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.956TyrAla: 1.956 ± 0.02
0.737TyrCys: 0.737 ± 0.013
1.884TyrAsp: 1.884 ± 0.019
2.08TyrGlu: 2.08 ± 0.023
1.323TyrPhe: 1.323 ± 0.017
2.075TyrGly: 2.075 ± 0.047
0.847TyrHis: 0.847 ± 0.011
1.735TyrIle: 1.735 ± 0.021
1.979TyrLys: 1.979 ± 0.023
2.864TyrLeu: 2.864 ± 0.029
0.756TyrMet: 0.756 ± 0.012
1.666TyrAsn: 1.666 ± 0.02
1.468TyrPro: 1.468 ± 0.023
1.211TyrGln: 1.211 ± 0.015
1.828TyrArg: 1.828 ± 0.021
2.366TyrSer: 2.366 ± 0.024
1.841TyrThr: 1.841 ± 0.02
2.031TyrVal: 2.031 ± 0.019
0.392TyrTrp: 0.392 ± 0.012
1.281TyrTyr: 1.281 ± 0.019
0.001TyrXaa: 0.001 ± 0.0
Xaa
0.001XaaAla: 0.001 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.002XaaAsp: 0.002 ± 0.001
0.001XaaGlu: 0.001 ± 0.0
0.002XaaPhe: 0.002 ± 0.001
0.002XaaGly: 0.002 ± 0.001
0.001XaaHis: 0.001 ± 0.0
0.001XaaIle: 0.001 ± 0.0
0.002XaaLys: 0.002 ± 0.0
0.003XaaLeu: 0.003 ± 0.001
0.002XaaMet: 0.002 ± 0.0
0.001XaaAsn: 0.001 ± 0.0
0.002XaaPro: 0.002 ± 0.001
0.001XaaGln: 0.001 ± 0.0
0.003XaaArg: 0.003 ± 0.001
0.003XaaSer: 0.003 ± 0.001
0.002XaaThr: 0.002 ± 0.001
0.001XaaVal: 0.001 ± 0.0
0.001XaaTrp: 0.001 ± 0.0
0.001XaaTyr: 0.001 ± 0.0
0.001XaaXaa: 0.001 ± 0.0
Statistics based on 14787 proteins (5961797 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski