Blautia sp. CAG:37

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Blautia; environmental samples

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2353 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7JTF7|R7JTF7_9FIRM UDP-glucose 4-epimerase OS=Blautia sp. CAG:37 OX=1262757 GN=BN630_02124 PE=3 SV=1
MM1 pKa = 7.3SKK3 pKa = 10.31VAVVYY8 pKa = 9.8WSGTGNTEE16 pKa = 3.56MMAQKK21 pKa = 10.15VAEE24 pKa = 4.31GAKK27 pKa = 9.75EE28 pKa = 3.67AGAEE32 pKa = 4.16VSVLTCADD40 pKa = 4.74FSADD44 pKa = 5.21DD45 pKa = 3.48VDD47 pKa = 5.35AYY49 pKa = 11.05DD50 pKa = 5.78AIAFGCPAMGAEE62 pKa = 4.18EE63 pKa = 5.23LEE65 pKa = 4.37DD66 pKa = 3.9TEE68 pKa = 4.43FEE70 pKa = 4.55PMFSACEE77 pKa = 3.98SKK79 pKa = 11.12LSGKK83 pKa = 10.1KK84 pKa = 8.94IALFGSYY91 pKa = 8.98GWGDD95 pKa = 4.2GEE97 pKa = 4.96WMRR100 pKa = 11.84TWEE103 pKa = 4.42EE104 pKa = 3.91SCKK107 pKa = 10.46NDD109 pKa = 4.16GAEE112 pKa = 4.15LVCDD116 pKa = 4.01SVICADD122 pKa = 4.19APDD125 pKa = 4.94DD126 pKa = 3.91EE127 pKa = 6.72AEE129 pKa = 4.21AACIALGKK137 pKa = 9.98ALCC140 pKa = 4.39

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7JS20|R7JS20_9FIRM Chain length determinant protein OS=Blautia sp. CAG:37 OX=1262757 GN=BN630_00008 PE=4 SV=1
MM1 pKa = 7.86RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84SCISGMAIKK13 pKa = 10.43RR14 pKa = 11.84CRR16 pKa = 11.84PVKK19 pKa = 10.82NKK21 pKa = 8.31ITYY24 pKa = 10.4LITKK28 pKa = 9.19RR29 pKa = 11.84LRR31 pKa = 11.84RR32 pKa = 11.84LTEE35 pKa = 3.61SLFFVIINAEE45 pKa = 3.71KK46 pKa = 10.71GIVIRR51 pKa = 11.84CGEE54 pKa = 4.22KK55 pKa = 9.66GRR57 pKa = 11.84SWGEE61 pKa = 3.36RR62 pKa = 11.84GVFDD66 pKa = 4.99GKK68 pKa = 10.57NISDD72 pKa = 3.84RR73 pKa = 11.84EE74 pKa = 4.04FFLPAEE80 pKa = 4.21VSGRR84 pKa = 11.84AGTSGGYY91 pKa = 10.38GKK93 pKa = 10.15IFGGNGFCGLYY104 pKa = 9.4IYY106 pKa = 10.4HH107 pKa = 6.64YY108 pKa = 9.86GRR110 pKa = 11.84RR111 pKa = 11.84HH112 pKa = 4.98IAGSGGRR119 pKa = 11.84ADD121 pKa = 4.0CGSGRR126 pKa = 11.84SAGVSYY132 pKa = 10.43KK133 pKa = 10.38RR134 pKa = 11.84RR135 pKa = 11.84IGEE138 pKa = 4.0PPDD141 pKa = 3.22RR142 pKa = 11.84CEE144 pKa = 3.82RR145 pKa = 11.84RR146 pKa = 11.84SASLCILPP154 pKa = 4.78

Molecular weight: 17.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2353

0

2353

754178

32

1615

320.5

35.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.058 ± 0.055

1.576 ± 0.023

5.431 ± 0.045

7.836 ± 0.065

4.123 ± 0.042

7.295 ± 0.043

1.875 ± 0.023

6.636 ± 0.048

6.415 ± 0.039

9.007 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.163 ± 0.024

3.938 ± 0.032

3.557 ± 0.029

3.381 ± 0.03

4.565 ± 0.041

5.599 ± 0.04

5.559 ± 0.043

7.032 ± 0.046

1.006 ± 0.019

3.944 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski