Bacillus sp. FJAT-22058

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M1NNZ7|A0A0M1NNZ7_9BACI ABC transporter domain-containing protein OS=Bacillus sp. FJAT-22058 OX=1581037 GN=AM233_07205 PE=3 SV=1
MM1 pKa = 7.76KK2 pKa = 9.95YY3 pKa = 10.7YY4 pKa = 10.4KK5 pKa = 10.42VSNSGFDD12 pKa = 2.9IKK14 pKa = 11.28VIVAYY19 pKa = 10.15SGYY22 pKa = 10.0EE23 pKa = 3.58ALGYY27 pKa = 10.76YY28 pKa = 10.59LMEE31 pKa = 6.61IDD33 pKa = 4.43DD34 pKa = 3.97QLGFVDD40 pKa = 5.16DD41 pKa = 4.19VDD43 pKa = 4.25VEE45 pKa = 4.36EE46 pKa = 4.41VDD48 pKa = 3.0ADD50 pKa = 3.62EE51 pKa = 4.64RR52 pKa = 11.84VEE54 pKa = 4.04ISYY57 pKa = 9.36TGYY60 pKa = 9.91PVYY63 pKa = 9.57KK64 pKa = 9.04TLKK67 pKa = 9.85EE68 pKa = 4.05LYY70 pKa = 9.38QEE72 pKa = 3.91KK73 pKa = 10.83DD74 pKa = 2.88FWQVPNVVVEE84 pKa = 4.47VEE86 pKa = 3.89

Molecular weight: 10.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M1NS70|A0A0M1NS70_9BACI Rhomboid domain-containing protein OS=Bacillus sp. FJAT-22058 OX=1581037 GN=AM233_12725 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.21QPNNRR10 pKa = 11.84KK11 pKa = 9.13HH12 pKa = 6.54SKK14 pKa = 8.73VHH16 pKa = 5.66GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 9.71NGRR28 pKa = 11.84RR29 pKa = 11.84VIAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4848

0

4848

1384686

26

2559

285.6

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.027 ± 0.041

0.733 ± 0.012

5.046 ± 0.032

7.419 ± 0.041

4.648 ± 0.029

7.141 ± 0.037

2.032 ± 0.018

8.136 ± 0.036

7.036 ± 0.038

9.821 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.89 ± 0.02

4.377 ± 0.026

3.607 ± 0.018

3.47 ± 0.025

3.879 ± 0.028

6.206 ± 0.029

5.299 ± 0.023

6.826 ± 0.023

1.016 ± 0.011

3.392 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski