Nitrospira moscoviensis

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4733 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K2GDB8|A0A0K2GDB8_NITMO Uncharacterized protein OS=Nitrospira moscoviensis OX=42253 GN=NITMOv2_2451 PE=4 SV=1
MM1 pKa = 7.4VLLCLCVMVQMLGVPATLLSPDD23 pKa = 4.18GPSDD27 pKa = 3.79LLGASVLEE35 pKa = 4.71GFALLPPVIDD45 pKa = 4.49LAPSSEE51 pKa = 4.57AEE53 pKa = 4.26TASDD57 pKa = 3.39TQYY60 pKa = 11.27VPLRR64 pKa = 11.84PLLITVPFHH73 pKa = 6.62PPVLSIFGG81 pKa = 3.59

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K2GBS3|A0A0K2GBS3_NITMO Uncharacterized protein OS=Nitrospira moscoviensis OX=42253 GN=NITMOv2_1904 PE=4 SV=1
MM1 pKa = 7.16MFRR4 pKa = 11.84RR5 pKa = 11.84GTGLIAGPPISRR17 pKa = 11.84PKK19 pKa = 9.3PALVTVATPTPPLISIPGSVRR40 pKa = 11.84SSTSAINSAPCVQSGSSPASLITLQIARR68 pKa = 11.84VSTRR72 pKa = 11.84SHH74 pKa = 6.56RR75 pKa = 11.84ARR77 pKa = 11.84AKK79 pKa = 7.36VTRR82 pKa = 11.84VRR84 pKa = 11.84FGSVVSMSDD93 pKa = 3.15SMVLLTRR100 pKa = 11.84QRR102 pKa = 11.84VAALAAAAAQAPVVYY117 pKa = 9.45PVRR120 pKa = 11.84KK121 pKa = 8.89PFRR124 pKa = 11.84LFGGTLLLIAHH135 pKa = 7.09LSVHH139 pKa = 4.59STRR142 pKa = 11.84RR143 pKa = 11.84SKK145 pKa = 10.43HH146 pKa = 4.51ARR148 pKa = 11.84RR149 pKa = 11.84LAARR153 pKa = 11.84SRR155 pKa = 11.84LL156 pKa = 3.65

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4733

0

4733

1377880

21

3578

291.1

32.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.654 ± 0.041

1.01 ± 0.015

5.237 ± 0.031

6.024 ± 0.038

3.517 ± 0.021

7.975 ± 0.042

2.395 ± 0.016

4.832 ± 0.025

3.764 ± 0.033

10.581 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.02

2.394 ± 0.025

5.437 ± 0.025

3.741 ± 0.023

7.602 ± 0.046

5.448 ± 0.026

5.421 ± 0.033

7.705 ± 0.029

1.399 ± 0.014

2.539 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski