Desulfuromonas thiophila

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfuromonadales; Desulfuromonadaceae; Desulfuromonas

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7DPW1|A0A1G7DPW1_9DELT Uncharacterized protein OS=Desulfuromonas thiophila OX=57664 GN=SAMN05661003_1155 PE=4 SV=1
MM1 pKa = 7.07STITDD6 pKa = 3.65YY7 pKa = 11.63YY8 pKa = 10.62KK9 pKa = 10.96YY10 pKa = 10.76SEE12 pKa = 4.79LALASYY18 pKa = 11.2SDD20 pKa = 4.14LAAGMVQQDD29 pKa = 4.3YY30 pKa = 11.21IDD32 pKa = 4.77ALMNDD37 pKa = 4.43GDD39 pKa = 5.08GMSQKK44 pKa = 9.85QAEE47 pKa = 4.49TFAPNWTVLDD57 pKa = 3.85QYY59 pKa = 11.72DD60 pKa = 3.74GMVEE64 pKa = 3.69EE65 pKa = 4.86TYY67 pKa = 11.12YY68 pKa = 11.22DD69 pKa = 3.58EE70 pKa = 6.29FGDD73 pKa = 3.79EE74 pKa = 4.15QTFLNPTGLSVTLFEE89 pKa = 5.87DD90 pKa = 4.59GSGKK94 pKa = 9.82QVVAIRR100 pKa = 11.84GTEE103 pKa = 3.88PTDD106 pKa = 3.89LDD108 pKa = 5.64DD109 pKa = 4.76IVTDD113 pKa = 4.66IIDD116 pKa = 3.6IGVLGTSEE124 pKa = 4.7HH125 pKa = 5.34QAQYY129 pKa = 11.21AALSAQIQKK138 pKa = 9.86WKK140 pKa = 10.69DD141 pKa = 3.21DD142 pKa = 3.48GTLRR146 pKa = 11.84TNFSVTGHH154 pKa = 5.93SLGGFLATNLARR166 pKa = 11.84EE167 pKa = 4.21DD168 pKa = 3.87CGILKK173 pKa = 10.44

Molecular weight: 18.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6Z5H5|A0A1G6Z5H5_9DELT Diguanylate cyclase (GGDEF) domain-containing protein OS=Desulfuromonas thiophila OX=57664 GN=SAMN05661003_102306 PE=4 SV=1
MM1 pKa = 7.46QAFLHH6 pKa = 5.52NLEE9 pKa = 4.28VEE11 pKa = 4.63RR12 pKa = 11.84NLSPHH17 pKa = 5.25SCRR20 pKa = 11.84AYY22 pKa = 10.49RR23 pKa = 11.84RR24 pKa = 11.84DD25 pKa = 3.24VGAFCLFLQQQLPGRR40 pKa = 11.84PLDD43 pKa = 3.94AQLAAVDD50 pKa = 3.71KK51 pKa = 10.82ALVRR55 pKa = 11.84RR56 pKa = 11.84WLGQLGRR63 pKa = 11.84RR64 pKa = 11.84NKK66 pKa = 10.2KK67 pKa = 8.24VTLARR72 pKa = 11.84KK73 pKa = 9.27QSALRR78 pKa = 11.84TFFTFLVRR86 pKa = 11.84RR87 pKa = 11.84GLLEE91 pKa = 3.57QHH93 pKa = 7.01PLHH96 pKa = 8.3SMEE99 pKa = 4.33RR100 pKa = 11.84PRR102 pKa = 11.84LEE104 pKa = 4.07RR105 pKa = 11.84PLPRR109 pKa = 11.84LLDD112 pKa = 3.55VDD114 pKa = 5.22AVFCLLDD121 pKa = 3.64GMVPEE126 pKa = 5.26DD127 pKa = 3.22WLRR130 pKa = 11.84WRR132 pKa = 11.84DD133 pKa = 3.27RR134 pKa = 11.84ALFEE138 pKa = 4.96LLYY141 pKa = 10.85SCGLRR146 pKa = 11.84VSEE149 pKa = 5.42LVALDD154 pKa = 4.15EE155 pKa = 5.41DD156 pKa = 5.41DD157 pKa = 5.45LRR159 pKa = 11.84ADD161 pKa = 3.28QGLVCVRR168 pKa = 11.84QGKK171 pKa = 9.55GGKK174 pKa = 8.04QRR176 pKa = 11.84IVPVGQAALRR186 pKa = 11.84ALQVYY191 pKa = 8.44LQHH194 pKa = 6.95CPPQKK199 pKa = 10.04RR200 pKa = 11.84VGVALFVNQRR210 pKa = 11.84GQRR213 pKa = 11.84LSVRR217 pKa = 11.84TVQRR221 pKa = 11.84NLKK224 pKa = 9.85AALARR229 pKa = 11.84AGLPTDD235 pKa = 3.91VSPHH239 pKa = 5.28GLRR242 pKa = 11.84HH243 pKa = 5.73SFATHH248 pKa = 6.67LLDD251 pKa = 4.8GGADD255 pKa = 3.22LRR257 pKa = 11.84AIQEE261 pKa = 4.44LLGHH265 pKa = 6.96ASLATTQRR273 pKa = 11.84YY274 pKa = 5.0TQVSGARR281 pKa = 11.84LAEE284 pKa = 4.64EE285 pKa = 4.42YY286 pKa = 10.85DD287 pKa = 3.52RR288 pKa = 11.84THH290 pKa = 6.76PRR292 pKa = 11.84SKK294 pKa = 10.61KK295 pKa = 9.74PSAA298 pKa = 3.65

Molecular weight: 33.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2471

0

2471

839046

41

6445

339.6

37.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.202 ± 0.065

1.491 ± 0.027

5.369 ± 0.07

5.736 ± 0.045

3.545 ± 0.032

7.598 ± 0.058

2.103 ± 0.028

4.719 ± 0.04

2.916 ± 0.048

12.935 ± 0.076

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.89 ± 0.024

2.653 ± 0.037

4.864 ± 0.047

5.466 ± 0.057

7.272 ± 0.063

5.256 ± 0.039

4.625 ± 0.056

6.76 ± 0.041

1.14 ± 0.017

2.461 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski