Eastern grey kangaroopox virus

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Macropopoxvirus; Eastern kangaroopox virus

Average proteome isoelectric point is 6.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C9DSW8|A0A2C9DSW8_9POXV Uncharacterized protein OS=Eastern grey kangaroopox virus OX=2042482 GN=EKPV-NSW-ORF010 PE=4 SV=1
MM1 pKa = 7.34FGIRR5 pKa = 11.84NTRR8 pKa = 11.84FCLSCEE14 pKa = 4.31TTNHH18 pKa = 4.37VNIYY22 pKa = 9.63SVSAEE27 pKa = 4.02VCYY30 pKa = 10.57LPPKK34 pKa = 10.32FLEE37 pKa = 4.23TLDD40 pKa = 4.56LCVQEE45 pKa = 5.22SDD47 pKa = 4.68CGCCYY52 pKa = 10.44KK53 pKa = 10.51SEE55 pKa = 4.42AFLNTPKK62 pKa = 9.88CTLPPHH68 pKa = 5.99ITCAFCDD75 pKa = 3.79TYY77 pKa = 11.55KK78 pKa = 10.81RR79 pKa = 11.84LHH81 pKa = 6.53KK82 pKa = 9.9PDD84 pKa = 3.75TGTSFQRR91 pKa = 11.84SVEE94 pKa = 4.1DD95 pKa = 4.0DD96 pKa = 3.73VQGTICGMEE105 pKa = 4.13EE106 pKa = 4.3DD107 pKa = 4.7DD108 pKa = 5.63VSILEE113 pKa = 4.07PAAIEE118 pKa = 4.25AEE120 pKa = 4.32TTEE123 pKa = 4.4EE124 pKa = 3.94EE125 pKa = 4.53VEE127 pKa = 4.1PEE129 pKa = 3.85EE130 pKa = 4.29TEE132 pKa = 4.26EE133 pKa = 3.96EE134 pKa = 4.46VEE136 pKa = 4.28PEE138 pKa = 3.87EE139 pKa = 4.41TEE141 pKa = 4.12AGAEE145 pKa = 3.95ALEE148 pKa = 4.34DD149 pKa = 4.02LLRR152 pKa = 11.84CDD154 pKa = 3.96EE155 pKa = 4.47SDD157 pKa = 3.99LIEE160 pKa = 5.22DD161 pKa = 3.71EE162 pKa = 5.28GVTFPHH168 pKa = 7.3DD169 pKa = 4.0KK170 pKa = 11.01KK171 pKa = 10.85NLPNMLYY178 pKa = 10.43ADD180 pKa = 4.26PEE182 pKa = 4.06AALMSEE188 pKa = 4.5EE189 pKa = 4.61EE190 pKa = 4.3YY191 pKa = 10.56MSFVNAEE198 pKa = 3.66LDD200 pKa = 3.76YY201 pKa = 10.61YY202 pKa = 11.51LEE204 pKa = 4.33VADD207 pKa = 5.55AEE209 pKa = 4.61VDD211 pKa = 3.9TEE213 pKa = 3.91WRR215 pKa = 11.84RR216 pKa = 11.84DD217 pKa = 3.62LEE219 pKa = 4.21RR220 pKa = 11.84LPGPVPEE227 pKa = 4.54FDD229 pKa = 4.9AVRR232 pKa = 11.84GWDD235 pKa = 3.57VDD237 pKa = 3.82PAGVWVAAGDD247 pKa = 3.94VVDD250 pKa = 5.97AEE252 pKa = 4.56EE253 pKa = 4.98DD254 pKa = 3.34WGQGPQAIWDD264 pKa = 3.89HH265 pKa = 6.32EE266 pKa = 4.52DD267 pKa = 3.06VGRR270 pKa = 11.84EE271 pKa = 3.98QEE273 pKa = 4.25WVPEE277 pKa = 4.02AGVVDD282 pKa = 4.18RR283 pKa = 11.84RR284 pKa = 11.84DD285 pKa = 3.56EE286 pKa = 3.86EE287 pKa = 4.24HH288 pKa = 7.21LIDD291 pKa = 5.14NRR293 pKa = 11.84YY294 pKa = 9.32DD295 pKa = 3.42HH296 pKa = 6.08YY297 pKa = 11.46AIWRR301 pKa = 11.84SIFDD305 pKa = 3.77EE306 pKa = 4.5RR307 pKa = 11.84QGGAPNEE314 pKa = 4.41DD315 pKa = 3.58EE316 pKa = 6.01DD317 pKa = 5.21EE318 pKa = 5.13IGDD321 pKa = 3.97SSFLCEE327 pKa = 5.29CDD329 pKa = 2.94TCEE332 pKa = 4.36YY333 pKa = 10.52ISDD336 pKa = 4.07PVQGHH341 pKa = 7.16LIIADD346 pKa = 3.44NDD348 pKa = 3.96GVVSVDD354 pKa = 3.54VLNGSHH360 pKa = 6.6TDD362 pKa = 3.61AVPSLCLAAYY372 pKa = 9.25LHH374 pKa = 6.33SCRR377 pKa = 11.84SGEE380 pKa = 3.92IEE382 pKa = 4.17FVKK385 pKa = 10.41HH386 pKa = 5.79YY387 pKa = 10.22IVQEE391 pKa = 4.04FFGGPPTSSIALRR404 pKa = 11.84RR405 pKa = 11.84CNN407 pKa = 3.5

Molecular weight: 45.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C9DSY8|A0A2C9DSY8_9POXV S-S bond formation pathway protein substrate OS=Eastern grey kangaroopox virus OX=2042482 GN=EKPV-NSW-ORF038 PE=4 SV=1
MM1 pKa = 7.09RR2 pKa = 11.84QLWRR6 pKa = 11.84LRR8 pKa = 11.84VLGAKK13 pKa = 9.92KK14 pKa = 10.35KK15 pKa = 10.99GGDD18 pKa = 4.05GDD20 pKa = 4.71RR21 pKa = 11.84GDD23 pKa = 4.52CGGGDD28 pKa = 3.56SSDD31 pKa = 3.51CLIYY35 pKa = 10.53SIYY38 pKa = 10.12RR39 pKa = 11.84VCNLPVRR46 pKa = 11.84QPVRR50 pKa = 11.84SGRR53 pKa = 11.84LARR56 pKa = 11.84RR57 pKa = 11.84WW58 pKa = 3.32

Molecular weight: 6.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

162

0

162

51353

32

2030

317.0

35.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.942 ± 0.259

2.206 ± 0.126

5.624 ± 0.116

6.368 ± 0.143

4.475 ± 0.148

5.419 ± 0.229

2.255 ± 0.097

5.388 ± 0.222

4.011 ± 0.181

9.762 ± 0.183

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.08

4.019 ± 0.164

4.539 ± 0.213

2.019 ± 0.07

7.55 ± 0.19

8.543 ± 0.266

5.396 ± 0.129

8.136 ± 0.146

0.61 ± 0.043

4.284 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski