Streptomyces sp. WMMB 714

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; unclassified Streptomyces

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5562 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C6PFX2|A0A1C6PFX2_9ACTN Argininosuccinate lyase OS=Streptomyces sp. WMMB 714 OX=1286822 GN=argH PE=3 SV=1
MM1 pKa = 7.16STGNDD6 pKa = 3.5ALEE9 pKa = 3.89VWIDD13 pKa = 3.4QDD15 pKa = 3.99LCTGDD20 pKa = 5.01GICAQYY26 pKa = 10.9APEE29 pKa = 4.23VFEE32 pKa = 5.9LDD34 pKa = 3.05IDD36 pKa = 3.71GLAYY40 pKa = 10.79VKK42 pKa = 10.44DD43 pKa = 3.94AQDD46 pKa = 3.32EE47 pKa = 4.19LLQEE51 pKa = 4.47KK52 pKa = 10.02GAATPVPLPLLNDD65 pKa = 3.56VVDD68 pKa = 4.2SAKK71 pKa = 10.28EE72 pKa = 3.92CPGDD76 pKa = 4.18CIHH79 pKa = 6.28VRR81 pKa = 11.84RR82 pKa = 11.84VSDD85 pKa = 3.47SVEE88 pKa = 4.23VYY90 pKa = 10.88GPDD93 pKa = 3.51ADD95 pKa = 5.36DD96 pKa = 3.62SAEE99 pKa = 3.85

Molecular weight: 10.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C6N506|A0A1C6N506_9ACTN Serine phosphatase RsbU regulator of sigma subunit OS=Streptomyces sp. WMMB 714 OX=1286822 GN=H181DRAFT_02044 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84LRR4 pKa = 11.84RR5 pKa = 11.84VVRR8 pKa = 11.84GLLGRR13 pKa = 11.84GEE15 pKa = 4.09PRR17 pKa = 11.84GGSWRR22 pKa = 11.84SAVRR26 pKa = 11.84MSRR29 pKa = 11.84LRR31 pKa = 11.84VRR33 pKa = 11.84LLVRR37 pKa = 11.84VGRR40 pKa = 11.84GLLSGLRR47 pKa = 11.84NVGRR51 pKa = 11.84GLPAVPRR58 pKa = 11.84LRR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84PRR64 pKa = 11.84LRR66 pKa = 11.84KK67 pKa = 9.73KK68 pKa = 10.4GALCQRR74 pKa = 11.84VLPGRR79 pKa = 11.84GLL81 pKa = 3.38

Molecular weight: 9.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5562

0

5562

1902022

29

4671

342.0

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.395 ± 0.054

0.83 ± 0.01

5.829 ± 0.029

6.308 ± 0.032

2.683 ± 0.017

10.061 ± 0.039

2.241 ± 0.016

2.79 ± 0.022

2.219 ± 0.033

10.095 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.789 ± 0.013

1.647 ± 0.016

6.234 ± 0.034

2.786 ± 0.019

8.269 ± 0.035

5.455 ± 0.023

5.64 ± 0.025

8.317 ± 0.027

1.437 ± 0.014

1.977 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski