Sphingomonas gellani

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3624 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H8HWX6|A0A1H8HWX6_9SPHN N-acetylgalactosamine-N N'-diacetylbacillosaminyl-diphospho-undecaprenol 4-alpha-N-acetylgalactosaminyltransferase OS=Sphingomonas gellani OX=1166340 GN=SAMN05192583_3131 PE=4 SV=1
MM1 pKa = 7.81IYY3 pKa = 10.43YY4 pKa = 10.27LDD6 pKa = 3.64VALTQSGIDD15 pKa = 3.5WANQVPIPTSMTKK28 pKa = 10.43AEE30 pKa = 4.19YY31 pKa = 9.98DD32 pKa = 3.6ALPDD36 pKa = 3.18KK37 pKa = 10.79TVFVRR42 pKa = 11.84DD43 pKa = 4.17GYY45 pKa = 9.8TIDD48 pKa = 4.09LSEE51 pKa = 4.3GTSTGFAYY59 pKa = 10.31EE60 pKa = 4.67DD61 pKa = 3.12IVGRR65 pKa = 11.84TVNQIRR71 pKa = 11.84GLFPDD76 pKa = 3.41EE77 pKa = 4.63NGDD80 pKa = 3.62YY81 pKa = 10.68DD82 pKa = 3.56HH83 pKa = 7.44TYY85 pKa = 11.3ARR87 pKa = 11.84VHH89 pKa = 5.58VAGAGDD95 pKa = 3.64FTVIGSSAGEE105 pKa = 3.99DD106 pKa = 3.7LYY108 pKa = 11.53GGTGNDD114 pKa = 3.29TLYY117 pKa = 11.16DD118 pKa = 3.74GGNAGFEE125 pKa = 4.28YY126 pKa = 10.55EE127 pKa = 4.28HH128 pKa = 7.51DD129 pKa = 4.15GLHH132 pKa = 6.63GGAGDD137 pKa = 3.55DD138 pKa = 3.42TYY140 pKa = 11.58YY141 pKa = 9.52ITGVDD146 pKa = 3.34SSVTEE151 pKa = 3.97KK152 pKa = 10.88AGEE155 pKa = 4.28GFDD158 pKa = 4.16RR159 pKa = 11.84VYY161 pKa = 11.65ADD163 pKa = 5.35GFFQLYY169 pKa = 8.96PDD171 pKa = 4.21SEE173 pKa = 4.74VEE175 pKa = 4.02FVKK178 pKa = 10.78ISGEE182 pKa = 3.92YY183 pKa = 10.04AQAFGNNLANIMVGSDD199 pKa = 3.45QPSIIAGEE207 pKa = 4.4GGNDD211 pKa = 3.51TLRR214 pKa = 11.84SDD216 pKa = 3.87GADD219 pKa = 2.94TVFEE223 pKa = 4.42GGVGNDD229 pKa = 3.28AMRR232 pKa = 11.84GGAGVDD238 pKa = 3.06VFLYY242 pKa = 10.36YY243 pKa = 10.76GKK245 pKa = 10.47EE246 pKa = 4.06DD247 pKa = 3.5GRR249 pKa = 11.84DD250 pKa = 3.35TISRR254 pKa = 11.84FGKK257 pKa = 10.21EE258 pKa = 4.03DD259 pKa = 3.5VLVTTSKK266 pKa = 10.65IADD269 pKa = 3.51ANGDD273 pKa = 3.84NIITFGSNKK282 pKa = 10.15ALDD285 pKa = 3.85FTATSSVRR293 pKa = 11.84ITDD296 pKa = 3.53DD297 pKa = 2.63TGATVKK303 pKa = 10.74ALEE306 pKa = 4.07YY307 pKa = 10.96DD308 pKa = 3.75GAFTDD313 pKa = 4.82GGTTYY318 pKa = 10.9YY319 pKa = 10.48VYY321 pKa = 11.16SRR323 pKa = 11.84VGSSAGVDD331 pKa = 3.37SALGSGLVTYY341 pKa = 8.6YY342 pKa = 11.04AQYY345 pKa = 10.32QQ346 pKa = 3.39

Molecular weight: 36.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H7Y3I2|A0A1H7Y3I2_9SPHN Tape measure domain-containing protein OS=Sphingomonas gellani OX=1166340 GN=SAMN05192583_0072 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84AVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3624

0

3624

1151458

24

2463

317.7

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.852 ± 0.063

0.727 ± 0.013

6.171 ± 0.031

4.772 ± 0.041

3.343 ± 0.025

9.185 ± 0.043

1.978 ± 0.022

4.443 ± 0.025

2.44 ± 0.03

9.733 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.282 ± 0.022

2.441 ± 0.036

5.547 ± 0.038

3.118 ± 0.025

7.955 ± 0.045

5.126 ± 0.032

5.657 ± 0.037

7.624 ± 0.032

1.457 ± 0.019

2.149 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski