Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium; Methylobacterium nodulans

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8II02|B8II02_METNO Aldehyde oxidase and xanthine dehydrogenase molybdopterin binding OS=Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060) OX=460265 GN=Mnod_1032 PE=4 SV=1
MM1 pKa = 7.3AVYY4 pKa = 10.31YY5 pKa = 10.23VDD7 pKa = 3.72SAAGSDD13 pKa = 4.02SNNGASTSTAFASIAKK29 pKa = 9.78VNSLNLQPGDD39 pKa = 3.41QVLFKK44 pKa = 10.59AGCSFSGTLAINASGTSTSRR64 pKa = 11.84IVIGSYY70 pKa = 9.2GTGADD75 pKa = 3.77PVFSSATTTIEE86 pKa = 4.02GGTAAIIVDD95 pKa = 4.01GNYY98 pKa = 10.22VSVSDD103 pKa = 3.86IKK105 pKa = 10.31ITSGSAAGIVVNGNWATVKK124 pKa = 10.58DD125 pKa = 4.1SEE127 pKa = 4.39ITGTGTGILVTGEE140 pKa = 3.79NGLYY144 pKa = 8.85TSNYY148 pKa = 7.09VHH150 pKa = 6.84DD151 pKa = 4.69TIMVRR156 pKa = 11.84NTQGTSAPDD165 pKa = 3.36NNDD168 pKa = 3.47DD169 pKa = 3.83YY170 pKa = 11.78GATAFSIAGSHH181 pKa = 6.26NEE183 pKa = 3.86FSFNTIDD190 pKa = 3.47DD191 pKa = 4.33CKK193 pKa = 10.65AASYY197 pKa = 10.86DD198 pKa = 3.8YY199 pKa = 10.92GWDD202 pKa = 3.28GGAFEE207 pKa = 4.24IWKK210 pKa = 8.6TVDD213 pKa = 4.58DD214 pKa = 5.51IYY216 pKa = 11.07IHH218 pKa = 7.59DD219 pKa = 3.94NTATDD224 pKa = 3.04SCGFIEE230 pKa = 4.61TGGEE234 pKa = 4.04TGDD237 pKa = 3.76TVSNLVIQNNLSKK250 pKa = 11.32DD251 pKa = 3.47NGGFLYY257 pKa = 9.65FHH259 pKa = 7.39NDD261 pKa = 2.58GGTFGVEE268 pKa = 3.78HH269 pKa = 7.22DD270 pKa = 4.54RR271 pKa = 11.84VDD273 pKa = 2.91ISYY276 pKa = 8.07NTIIEE281 pKa = 4.32SDD283 pKa = 3.51PDD285 pKa = 5.08AILVGTDD292 pKa = 3.56GPVGSEE298 pKa = 3.43MDD300 pKa = 3.45FHH302 pKa = 8.22NNIVVASDD310 pKa = 3.74GASHH314 pKa = 6.24QGWVVFNEE322 pKa = 4.25PWSSRR327 pKa = 11.84SYY329 pKa = 11.22NFYY332 pKa = 10.83EE333 pKa = 4.84GDD335 pKa = 3.69VEE337 pKa = 4.2TDD339 pKa = 3.17QVNGVAVDD347 pKa = 4.2DD348 pKa = 4.12LTATEE353 pKa = 3.97VRR355 pKa = 11.84GDD357 pKa = 3.51VGFVSSTDD365 pKa = 3.81FHH367 pKa = 6.5TLGTDD372 pKa = 3.55GLGMGSYY379 pKa = 9.84GAQGQTSSGTDD390 pKa = 3.19GNDD393 pKa = 2.95AVQLGFGSDD402 pKa = 3.72VYY404 pKa = 11.49NGGLGNDD411 pKa = 4.56TINGGLGNDD420 pKa = 4.47TINGGGGNDD429 pKa = 3.73VIEE432 pKa = 4.87GGAGFDD438 pKa = 3.64WLTGSAGSDD447 pKa = 2.97TFVFNGDD454 pKa = 4.18TISSTGIDD462 pKa = 4.48HH463 pKa = 6.9ITDD466 pKa = 4.01LNSADD471 pKa = 4.49DD472 pKa = 4.53MIDD475 pKa = 3.14LGEE478 pKa = 4.51AFSSLSKK485 pKa = 9.8GTPPASEE492 pKa = 4.01FRR494 pKa = 11.84AGTDD498 pKa = 3.17AQDD501 pKa = 3.88PDD503 pKa = 4.67DD504 pKa = 4.31YY505 pKa = 11.49LIYY508 pKa = 10.65DD509 pKa = 3.7QGAGRR514 pKa = 11.84LWYY517 pKa = 10.04DD518 pKa = 3.0ADD520 pKa = 4.27GNGGGYY526 pKa = 9.12KK527 pKa = 9.9QLIATFEE534 pKa = 4.16RR535 pKa = 11.84GTYY538 pKa = 9.31LDD540 pKa = 4.35RR541 pKa = 11.84WNFWVFF547 pKa = 3.02

Molecular weight: 56.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B8IIB2|B8IIB2_METNO Type VI secretion protein EvpB/VC_A0108 family OS=Methylobacterium nodulans (strain LMG 21967 / CNCM I-2342 / ORS 2060) OX=460265 GN=Mnod_3040 PE=4 SV=1
MM1 pKa = 8.0LIRR4 pKa = 11.84TAFSASVRR12 pKa = 11.84RR13 pKa = 11.84VTVRR17 pKa = 11.84FVKK20 pKa = 10.47RR21 pKa = 11.84QGSGKK26 pKa = 10.01SKK28 pKa = 10.27PRR30 pKa = 11.84LHH32 pKa = 7.31PKK34 pKa = 9.92RR35 pKa = 4.12

Molecular weight: 4.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8128

0

8128

2306247

30

4120

283.7

30.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.101 ± 0.047

0.914 ± 0.009

5.352 ± 0.023

5.538 ± 0.029

3.347 ± 0.019

8.872 ± 0.026

1.977 ± 0.014

4.451 ± 0.022

2.396 ± 0.024

10.461 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.013

2.08 ± 0.02

5.971 ± 0.032

2.912 ± 0.018

8.444 ± 0.038

4.99 ± 0.027

5.266 ± 0.028

7.479 ± 0.024

1.339 ± 0.014

2.06 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski